Values CatLog read from this paper
Mutational analysis of the active site flap (20s loop) of mandelate racemase
PMID 18092808doi:10.1021/bi7015525Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 60 records (180 values) from this paper: mandelate racemase from Pseudomonas putida.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| mandelate racemase | Pseudomonas putida | (R)-2-naphthylglycolate3.3 mM MgCl2 | 46 | 1.2 | 38 | pH 7.5, 25 °C | Open record: mandelate racemase, (R)-2-naphthylglycolate |
| mandelate racemase | Pseudomonas putida | (R)-2-naphthylglycolate20 mM MgCl2 | 70 | 3.5 | 20.1 | pH 7.5, 25 °C | Open record: mandelate racemase, (R)-2-naphthylglycolate |
| mandelate racemase | Pseudomonas putida | (R)-mandelate3.3 mM MgCl2 | 552 | 1.2 | 460 | pH 7.5, 25 °C | Open record: mandelate racemase, (R)-mandelate |
| mandelate racemase | Pseudomonas putida | (R)-mandelate20 mM MgCl2 | 620 | 1.47 | 430 | pH 7.5, 25 °C | Open record: mandelate racemase, (R)-mandelate |
| mandelate racemase | Pseudomonas putida | (S)-2-naphthylglycolate3.3 mM MgCl2 | 32 | 1 | 31 | pH 7.5, 25 °C | Open record: mandelate racemase, (S)-2-naphthylglycolate |
| mandelate racemase | Pseudomonas putida | (S)-2-naphthylglycolate20 mM MgCl2 | 38.4 | 1.6 | 24 | pH 7.5, 25 °C | Open record: mandelate racemase, (S)-2-naphthylglycolate |
| mandelate racemase | Pseudomonas putida | (S)-mandelate3.3 mM MgCl2 | 470 | 0.97 | 480 | pH 7.5, 25 °C | Open record: mandelate racemase, (S)-mandelate |
| mandelate racemase | Pseudomonas putida | (S)-mandelate20 mM MgCl2 | 492 | 1.07 | 460 | pH 7.5, 25 °C | Open record: mandelate racemase, (S)-mandelate |
| mandelate racemasevariant A25V | Pseudomonas putida | (R)-2-naphthylglycolate | 5.4 | 1.9 | 2.9 | pH 7.5, 25 °C | Open record: mandelate racemase variant A25V, (R)-2-naphthylglycolate |
| mandelate racemasevariant A25V | Pseudomonas putida | (R)-mandelate | 13 | 1.1 | 12 | pH 7.5, 25 °C | Open record: mandelate racemase variant A25V, (R)-mandelate |
| mandelate racemasevariant A25V | Pseudomonas putida | (S)-2-naphthylglycolate | 5.8 | 1.67 | 3.5 | pH 7.5, 25 °C | Open record: mandelate racemase variant A25V, (S)-2-naphthylglycolate |
| mandelate racemasevariant A25V | Pseudomonas putida | (S)-mandelate | 22 | 1.8 | 12 | pH 7.5, 25 °C | Open record: mandelate racemase variant A25V, (S)-mandelate |
| mandelate racemasevariant T24S | Pseudomonas putida | (R)-2-naphthylglycolate | 18.3 | 3.2 | 5.7 | pH 7.5, 25 °C | Open record: mandelate racemase variant T24S, (R)-2-naphthylglycolate |
| mandelate racemasevariant T24S | Pseudomonas putida | (R)-mandelate | 102 | 2.8 | 37 | pH 7.5, 25 °C | Open record: mandelate racemase variant T24S, (R)-mandelate |
| mandelate racemasevariant T24S | Pseudomonas putida | (S)-2-naphthylglycolate | 8.9 | 1.4 | 6.6 | pH 7.5, 25 °C | Open record: mandelate racemase variant T24S, (S)-2-naphthylglycolate |
| mandelate racemasevariant T24S | Pseudomonas putida | (S)-mandelate | 120 | 3 | 36 | pH 7.5, 25 °C | Open record: mandelate racemase variant T24S, (S)-mandelate |
| mandelate racemasevariant V22A | Pseudomonas putida | (R)-2-naphthylglycolate | 15.6 | 1.9 | 8.1 | pH 7.5, 25 °C | Open record: mandelate racemase variant V22A, (R)-2-naphthylglycolate |
| mandelate racemasevariant V22A | Pseudomonas putida | (R)-mandelate | 82 | 4.4 | 19 | pH 7.5, 25 °C | Open record: mandelate racemase variant V22A, (R)-mandelate |
| mandelate racemasevariant V22A | Pseudomonas putida | (S)-2-naphthylglycolate | 11.1 | 1.55 | 7.2 | pH 7.5, 25 °C | Open record: mandelate racemase variant V22A, (S)-2-naphthylglycolate |
| mandelate racemasevariant V22A | Pseudomonas putida | (S)-mandelate | 77 | 3.4 | 23 | pH 7.5, 25 °C | Open record: mandelate racemase variant V22A, (S)-mandelate |
| mandelate racemasevariant V22F | Pseudomonas putida | (R)-2-naphthylglycolate | 10.4 | 2.2 | 4.7 | pH 7.5, 25 °C | Open record: mandelate racemase variant V22F, (R)-2-naphthylglycolate |
| mandelate racemasevariant V22F | Pseudomonas putida | (R)-mandelate | 98 | 4.4 | 22 | pH 7.5, 25 °C | Open record: mandelate racemase variant V22F, (R)-mandelate |
| mandelate racemasevariant V22F | Pseudomonas putida | (S)-2-naphthylglycolate | 2.95 | 0.62 | 4.8 | pH 7.5, 25 °C | Open record: mandelate racemase variant V22F, (S)-2-naphthylglycolate |
| mandelate racemasevariant V22F | Pseudomonas putida | (S)-mandelate | 98 | 6.1 | 16 | pH 7.5, 25 °C | Open record: mandelate racemase variant V22F, (S)-mandelate |
| mandelate racemasevariant V22I | Pseudomonas putida | (R)-2-naphthylglycolate | 147 | 4.2 | 34 | pH 7.5, 25 °C | Open record: mandelate racemase variant V22I, (R)-2-naphthylglycolate |
| mandelate racemasevariant V22I | Pseudomonas putida | (R)-mandelate | 1.15 × 10³ | 2.9 | 400 | pH 7.5, 25 °C | Open record: mandelate racemase variant V22I, (R)-mandelate |
| mandelate racemasevariant V22I | Pseudomonas putida | (S)-2-naphthylglycolate | 101 | 2.9 | 35 | pH 7.5, 25 °C | Open record: mandelate racemase variant V22I, (S)-2-naphthylglycolate |
| mandelate racemasevariant V22I | Pseudomonas putida | (S)-mandelate | 740 | 1.7 | 440 | pH 7.5, 25 °C | Open record: mandelate racemase variant V22I, (S)-mandelate |
| mandelate racemasevariant V22I/V29L | Pseudomonas putida | (R)-2-naphthylglycolate | 10.5 | 3.7 | 2.9 | pH 7.5, 25 °C | Open record: mandelate racemase variant V22I/V29L, (R)-2-naphthylglycolate |
| mandelate racemasevariant V22I/V29L | Pseudomonas putida | (R)-mandelate | 1.01 × 10³ | 2.8 | 370 | pH 7.5, 25 °C | Open record: mandelate racemase variant V22I/V29L, (R)-mandelate |
| mandelate racemasevariant V22I/V29L | Pseudomonas putida | (S)-2-naphthylglycolate | 7.1 | 2.4 | 2.9 | pH 7.5, 25 °C | Open record: mandelate racemase variant V22I/V29L, (S)-2-naphthylglycolate |
| mandelate racemasevariant V22I/V29L | Pseudomonas putida | (S)-mandelate | 710 | 1.5 | 470 | pH 7.5, 25 °C | Open record: mandelate racemase variant V22I/V29L, (S)-mandelate |
| mandelate racemasevariant V26A | Pseudomonas putida | (R)-2-naphthylglycolate | 30 | 2.1 | 14 | pH 7.5, 25 °C | Open record: mandelate racemase variant V26A, (R)-2-naphthylglycolate |
| mandelate racemasevariant V26A | Pseudomonas putida | (R)-mandelate | 304 | 0.91 | 330 | pH 7.5, 25 °C | Open record: mandelate racemase variant V26A, (R)-mandelate |
| mandelate racemasevariant V26A | Pseudomonas putida | (S)-2-naphthylglycolate | 14 | 0.74 | 19 | pH 7.5, 25 °C | Open record: mandelate racemase variant V26A, (S)-2-naphthylglycolate |
| mandelate racemasevariant V26A | Pseudomonas putida | (S)-mandelate | 550 | 1.7 | 330 | pH 7.5, 25 °C | Open record: mandelate racemase variant V26A, (S)-mandelate |
| mandelate racemasevariant V26A/V29L | Pseudomonas putida | (R)-2-naphthylglycolate | 23 | 4.4 | 5.2 | pH 7.5, 25 °C | Open record: mandelate racemase variant V26A/V29L, (R)-2-naphthylglycolate |
| mandelate racemasevariant V26A/V29L | Pseudomonas putida | (R)-mandelate | 106 | 1.4 | 75 | pH 7.5, 25 °C | Open record: mandelate racemase variant V26A/V29L, (R)-mandelate |
| mandelate racemasevariant V26A/V29L | Pseudomonas putida | (S)-2-naphthylglycolate | 9.8 | 1.7 | 5.9 | pH 7.5, 25 °C | Open record: mandelate racemase variant V26A/V29L, (S)-2-naphthylglycolate |
| mandelate racemasevariant V26A/V29L | Pseudomonas putida | (S)-mandelate | 89 | 1.1 | 84 | pH 7.5, 25 °C | Open record: mandelate racemase variant V26A/V29L, (S)-mandelate |
| mandelate racemasevariant V26F | Pseudomonas putida | (R)-2-naphthylglycolate | 7.3 | 1.7 | 4.4 | pH 7.5, 25 °C | Open record: mandelate racemase variant V26F, (R)-2-naphthylglycolate |
| mandelate racemasevariant V26F | Pseudomonas putida | (R)-mandelate | 33 | 1.8 | 18 | pH 7.5, 25 °C | Open record: mandelate racemase variant V26F, (R)-mandelate |
| mandelate racemasevariant V26F | Pseudomonas putida | (S)-2-naphthylglycolate | 5.4 | 1.1 | 5.1 | pH 7.5, 25 °C | Open record: mandelate racemase variant V26F, (S)-2-naphthylglycolate |
| mandelate racemasevariant V26F | Pseudomonas putida | (S)-mandelate | 22 | 1.1 | 21 | pH 7.5, 25 °C | Open record: mandelate racemase variant V26F, (S)-mandelate |
| mandelate racemasevariant V26L | Pseudomonas putida | (R)-2-naphthylglycolate | 14 | 1.3 | 11 | pH 7.5, 25 °C | Open record: mandelate racemase variant V26L, (R)-2-naphthylglycolate |
| mandelate racemasevariant V26L | Pseudomonas putida | (R)-mandelate | 36 | 1.7 | 21 | pH 7.5, 25 °C | Open record: mandelate racemase variant V26L, (R)-mandelate |
| mandelate racemasevariant V26L | Pseudomonas putida | (S)-2-naphthylglycolate | 8.2 | 0.62 | 13 | pH 7.5, 25 °C | Open record: mandelate racemase variant V26L, (S)-2-naphthylglycolate |
| mandelate racemasevariant V26L | Pseudomonas putida | (S)-mandelate | 23 | 1.1 | 21 | pH 7.5, 25 °C | Open record: mandelate racemase variant V26L, (S)-mandelate |
| mandelate racemasevariant V29A | Pseudomonas putida | (R)-2-naphthylglycolate | 53 | 1.8 | 31 | pH 7.5, 25 °C | Open record: mandelate racemase variant V29A, (R)-2-naphthylglycolate |
| mandelate racemasevariant V29A | Pseudomonas putida | (R)-mandelate | 240 | 5 | 44 | pH 7.5, 25 °C | Open record: mandelate racemase variant V29A, (R)-mandelate |
| mandelate racemasevariant V29A | Pseudomonas putida | (S)-2-naphthylglycolate | 30 | 0.93 | 32 | pH 7.5, 25 °C | Open record: mandelate racemase variant V29A, (S)-2-naphthylglycolate |
| mandelate racemasevariant V29A | Pseudomonas putida | (S)-mandelate | 176 | 3.9 | 45 | pH 7.5, 25 °C | Open record: mandelate racemase variant V29A, (S)-mandelate |
| mandelate racemasevariant V29F | Pseudomonas putida | (R)-2-naphthylglycolate | 14.1 | 1.1 | 14 | pH 7.5, 25 °C | Open record: mandelate racemase variant V29F, (R)-2-naphthylglycolate |
| mandelate racemasevariant V29F | Pseudomonas putida | (R)-mandelate | 53 | 0.9 | 57 | pH 7.5, 25 °C | Open record: mandelate racemase variant V29F, (R)-mandelate |
| mandelate racemasevariant V29F | Pseudomonas putida | (S)-2-naphthylglycolate | 9.7 | 0.55 | 18 | pH 7.5, 25 °C | Open record: mandelate racemase variant V29F, (S)-2-naphthylglycolate |
| mandelate racemasevariant V29F | Pseudomonas putida | (S)-mandelate | 53 | 1.1 | 50 | pH 7.5, 25 °C | Open record: mandelate racemase variant V29F, (S)-mandelate |
| mandelate racemasevariant V29L | Pseudomonas putida | (R)-2-naphthylglycolate | 137 | 1.6 | 85 | pH 7.5, 25 °C | Open record: mandelate racemase variant V29L, (R)-2-naphthylglycolate |
| mandelate racemasevariant V29L | Pseudomonas putida | (R)-mandelate | 300 | 1.7 | 260 | pH 7.5, 25 °C | Open record: mandelate racemase variant V29L, (R)-mandelate |
| mandelate racemasevariant V29L | Pseudomonas putida | (S)-2-naphthylglycolate | 65 | 0.8 | 81 | pH 7.5, 25 °C | Open record: mandelate racemase variant V29L, (S)-2-naphthylglycolate |
| mandelate racemasevariant V29L | Pseudomonas putida | (S)-mandelate | 180 | 0.6 | 290 | pH 7.5, 25 °C | Open record: mandelate racemase variant V29L, (S)-mandelate |