Values CatLog read from this paper
Steady-state and pre-steady-state kinetic evaluation of severe acute respiratory syndrome coronavirus (SARS-CoV) 3CLpro cysteine protease: development of an ion-pair model for catalysis
PMID 18237196doi:10.1021/bi702107vOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 14 records (29 values) from this paper: 3C-like protease (3CLpro) from severe acute respiratory syndrome coronavirus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 3C-like protease (3CLpro) | severe acute respiratory syndrome coronavirus | Dabcyl-KTSAVLQSGFRKME-EdansFRET assay (Dabcyl/Edans): 20 mM BTP; 1 mM EDTA; H2O | 1.5 | 0.045 | 34 | pH 7 | Open record: 3C-like protease (3CLpro), Dabcyl-KTSAVLQSGFRKME-Edans |
| 3C-like protease (3CLpro) | severe acute respiratory syndrome coronavirus | Dabcyl-KTSAVLQSGFRKME-EdansFRET assay (Dabcyl/Edans): 20 mM BTP; 1 mM EDTA; H2O | — | — | 34 | pH 7 | Open record: 3C-like protease (3CLpro), Dabcyl-KTSAVLQSGFRKME-Edans |
| 3C-like protease (3CLpro) | severe acute respiratory syndrome coronavirus | Dabcyl-KTSAVLQSGFRKME-EdansFRET assay (Dabcyl/Edans) in D2O: 20 mM [2H]-BTP; Table 2 | 3.7 | 0.044 | 90 | pH 7 | Open record: 3C-like protease (3CLpro), Dabcyl-KTSAVLQSGFRKME-Edans |
| 3C-like protease (3CLpro) | severe acute respiratory syndrome coronavirus | Dabcyl-KTSAVLQSGFRKME-EdansFRET assay (Dabcyl/Edans) in D2O: 20 mM [2H]-BTP | — | — | 60 | pH 7 | Open record: 3C-like protease (3CLpro), Dabcyl-KTSAVLQSGFRKME-Edans |
| 3C-like protease (3CLpro) | severe acute respiratory syndrome coronavirus | SITSAVLQ-pNA (amide)colorimetric assay (pNA release, 400 nm): 12 mM BTP; H2O | 0.86 | 0.18 | 4.8 | pH 7 | Open record: 3C-like protease (3CLpro), SITSAVLQ-pNA (amide) |
| 3C-like protease (3CLpro) | severe acute respiratory syndrome coronavirus | SITSAVLQ-pNA (amide)colorimetric assay (pNA release, 400 nm): 12 mM BTP; H2O | — | — | 5 | pH 7 | Open record: 3C-like protease (3CLpro), SITSAVLQ-pNA (amide) |
| 3C-like protease (3CLpro) | severe acute respiratory syndrome coronavirus | SITSAVLQ-pNA (amide)colorimetric assay (pNA release, 400 nm) in D2O: [2H]-BTP | 0.98 | 0.175 | 5.6 | pH 7 | Open record: 3C-like protease (3CLpro), SITSAVLQ-pNA (amide) |
| 3C-like protease (3CLpro) | severe acute respiratory syndrome coronavirus | SITSAVLQ-pNA (amide)colorimetric assay (pNA release, 400 nm) in D2O: [2H]-BTP | — | — | 6.8 | pH 7 | Open record: 3C-like protease (3CLpro), SITSAVLQ-pNA (amide) |
| 3C-like protease (3CLpro) | severe acute respiratory syndrome coronavirus | SITSAVLQ-pNP (ester)colorimetric assay (pNP release, 400 nm): 20 mM HEPES; H2O | 0.6 | 0.073 | 8.3 | pH 7 | Open record: 3C-like protease (3CLpro), SITSAVLQ-pNP (ester) |
| 3C-like protease (3CLpro) | severe acute respiratory syndrome coronavirus | SITSAVLQ-pNP (ester)colorimetric assay (pNP release, 400 nm) in D2O: [2H]-HEPES | 0.47 | 0.074 | 6.5 | pH 7 | Open record: 3C-like protease (3CLpro), SITSAVLQ-pNP (ester) |
| 3C-like protease (3CLpro) | severe acute respiratory syndrome coronavirus | SITSAVLQ-pNP (ester) | 0.43 | 0.073 | 5.9 calc. | pH 7, 25 °C | Open record: 3C-like protease (3CLpro), SITSAVLQ-pNP (ester) |
| 3C-like protease (3CLpro) | severe acute respiratory syndrome coronavirus | SITSAVLQSGFRKMA | 8.5 | 0.6 | 14 | pH 7.5, 25 °C | Open record: 3C-like protease (3CLpro), SITSAVLQSGFRKMA |
| 3C-like protease (3CLpro) | severe acute respiratory syndrome coronavirus | Tamra-SITSAVLQSGFRKMAK-(Dabcyl)-OHFRET assay (Tamra/Dabcyl): 20 mM HEPES; 1 mM EDTA; H2O | — | — | 14 | pH 7 | Open record: 3C-like protease (3CLpro), Tamra-SITSAVLQSGFRKMAK-(Dabcyl)-OH |
| 3C-like protease (3CLpro) | severe acute respiratory syndrome coronavirus | Tamra-SITSAVLQSGFRKMAK-(Dabcyl)-OHFRET assay (Tamra/Dabcyl) in D2O: 20 mM [2H]-HEPES | — | — | 24 | pH 7 | Open record: 3C-like protease (3CLpro), Tamra-SITSAVLQSGFRKMAK-(Dabcyl)-OH |