Values CatLog read from this paper
Mutagenesis studies of substrate recognition and catalysis in the sortase A transpeptidase from Staphylococcus aureus
PMID 18375951doi:10.1074/jbc.m800974200Open in Browse
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CatLog read 11 records (33 values) from this paper: sortase A (SrtA) from Staphylococcus aureus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| sortase A (SrtA) | Staphylococcus aureus | aminobenzoyl-Leu-Pro-Glu-Thr-Gly-Gly-diaminopropionic acid(dinitrophenyl)-amiderecombinant | 1.1 | 8.76 | 0.125 | pH 7.5, 37 °C | Open record: sortase A (SrtA), aminobenzoyl-Leu-Pro-Glu-Thr-Gly-Gly-diaminopropionic acid(dinitrophenyl)-amide |
| sortase A (SrtA) | Staphylococcus aureus | aminobenzoyl-Leu-Pro-Glu-Thr-Gly-Gly-diaminopropionic acid(dinitrophenyl)-amidethen refolded | 0.67 | 11.2 | 0.0597 | pH 7.5, 37 °C | Open record: sortase A (SrtA), aminobenzoyl-Leu-Pro-Glu-Thr-Gly-Gly-diaminopropionic acid(dinitrophenyl)-amide |
| sortase A (SrtA)variant D170A | Staphylococcus aureus | aminobenzoyl-Leu-Pro-Glu-Thr-Gly-Gly-diaminopropionic acid(dinitrophenyl)-amide | 1.09 | 8.13 | 0.133 | pH 7.5, 37 °C | Open record: sortase A (SrtA) variant D170A, aminobenzoyl-Leu-Pro-Glu-Thr-Gly-Gly-diaminopropionic acid(dinitrophenyl)-amide |
| sortase A (SrtA)variant E171A | Staphylococcus aureus | aminobenzoyl-Leu-Pro-Glu-Thr-Gly-Gly-diaminopropionic acid(dinitrophenyl)-amide | 0.16 | 6.74 | 0.0231 | pH 7.5, 37 °C | Open record: sortase A (SrtA) variant E171A, aminobenzoyl-Leu-Pro-Glu-Thr-Gly-Gly-diaminopropionic acid(dinitrophenyl)-amide |
| sortase A (SrtA)variant G167A | Staphylococcus aureus | aminobenzoyl-Leu-Pro-Glu-Thr-Gly-Gly-diaminopropionic acid(dinitrophenyl)-amide | 1.13 | 8.7 | 0.129 | pH 7.5, 37 °C | Open record: sortase A (SrtA) variant G167A, aminobenzoyl-Leu-Pro-Glu-Thr-Gly-Gly-diaminopropionic acid(dinitrophenyl)-amide |
| sortase A (SrtA)variant L169A | Staphylococcus aureus | aminobenzoyl-Leu-Pro-Glu-Thr-Gly-Gly-diaminopropionic acid(dinitrophenyl)-amide | 0.0123 | 9.14 | 0.00135 | pH 7.5, 37 °C | Open record: sortase A (SrtA) variant L169A, aminobenzoyl-Leu-Pro-Glu-Thr-Gly-Gly-diaminopropionic acid(dinitrophenyl)-amide |
| sortase A (SrtA)variant Q172A | Staphylococcus aureus | aminobenzoyl-Leu-Pro-Glu-Thr-Gly-Gly-diaminopropionic acid(dinitrophenyl)-amide | 1.13 | 12.7 | 0.0893 | pH 7.5, 37 °C | Open record: sortase A (SrtA) variant Q172A, aminobenzoyl-Leu-Pro-Glu-Thr-Gly-Gly-diaminopropionic acid(dinitrophenyl)-amide |
| sortase A (SrtA)variant R197A | Staphylococcus aureus | aminobenzoyl-Leu-Pro-Glu-Thr-Gly-Gly-diaminopropionic acid(dinitrophenyl)-amide | 6.28 × 10⁻⁴ | 4.69 | 1.3 × 10⁻⁴ | pH 7.5, 37 °C | Open record: sortase A (SrtA) variant R197A, aminobenzoyl-Leu-Pro-Glu-Thr-Gly-Gly-diaminopropionic acid(dinitrophenyl)-amide |
| sortase A (SrtA)variant R197Cit | Staphylococcus aureus | aminobenzoyl-Leu-Pro-Glu-Thr-Gly-Gly-diaminopropionic acid(dinitrophenyl)-amide | 0.29 | 13.7 | 0.0211 | pH 7.5, 37 °C | Open record: sortase A (SrtA) variant R197Cit, aminobenzoyl-Leu-Pro-Glu-Thr-Gly-Gly-diaminopropionic acid(dinitrophenyl)-amide |
| sortase A (SrtA)variant R197K | Staphylococcus aureus | aminobenzoyl-Leu-Pro-Glu-Thr-Gly-Gly-diaminopropionic acid(dinitrophenyl)-amide | 0.0019 | 10.4 | 1.8 × 10⁻⁴ | pH 7.5, 37 °C | Open record: sortase A (SrtA) variant R197K, aminobenzoyl-Leu-Pro-Glu-Thr-Gly-Gly-diaminopropionic acid(dinitrophenyl)-amide |
| sortase A (SrtA)variant V168A | Staphylococcus aureus | aminobenzoyl-Leu-Pro-Glu-Thr-Gly-Gly-diaminopropionic acid(dinitrophenyl)-amide | 0.15 | 6.56 | 0.0227 | pH 7.5, 37 °C | Open record: sortase A (SrtA) variant V168A, aminobenzoyl-Leu-Pro-Glu-Thr-Gly-Gly-diaminopropionic acid(dinitrophenyl)-amide |