Values CatLog read from this paper
Hydrophobic repacking of the dimer interface of triosephosphate isomerase by in silico design and directed evolution
PMID 18439027doi:10.1021/bi702502kOpen in Browse
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CatLog read 4 records (12 values) from this paper: triosephosphate isomerase from Saccharomyces cerevisiae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| triosephosphate isomerasevariant K17A/Y46A/D48F/Q82A/D85S | Saccharomyces cerevisiae | D-glyceraldehyde 3-phosphate | 7.02 × 10³ | 1.09 | 6.44 × 10³ | pH 7.4, 25 °C | Open record: triosephosphate isomerase variant K17A/Y46A/D48F/Q82A/D85S, D-glyceraldehyde 3-phosphate |
| triosephosphate isomerasevariant K17L/Y46F/D48F/Q82F/D85L | Saccharomyces cerevisiae | D-glyceraldehyde 3-phosphate | 0.69 | 1.2 | 0.573 | pH 7.4, 25 °C | Open record: triosephosphate isomerase variant K17L/Y46F/D48F/Q82F/D85L, D-glyceraldehyde 3-phosphate |
| triosephosphate isomerasevariant K17L/Y46F/D48Y/Q82A/D85A | Saccharomyces cerevisiae | D-glyceraldehyde 3-phosphate | 1.04 | 1.26 | 0.825 | pH 7.4, 25 °C | Open record: triosephosphate isomerase variant K17L/Y46F/D48Y/Q82A/D85A, D-glyceraldehyde 3-phosphate |
| triosephosphate isomerasevariant K17P/Y46A/D48L/Q82T/D85A | Saccharomyces cerevisiae | D-glyceraldehyde 3-phosphate | 4.16 × 10³ | 2.44 | 1.64 × 10³ | pH 7.4, 25 °C | Open record: triosephosphate isomerase variant K17P/Y46A/D48L/Q82T/D85A, D-glyceraldehyde 3-phosphate |