Values CatLog read from this paper
Mutational analysis of the zinc- and substrate-binding sites in the CphA metallo-beta-lactamase from Aeromonas hydrophila
PMID 18498253doi:10.1042/bj20080375Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 55 records (137 values) from this paper: CphA metallo-beta-lactamase from Aeromonas hydrophila.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| CphA metallo-beta-lactamasevariant D120A | Aeromonas hydrophila | biapenem | 0.0015 | 0.1 | 0.015 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant D120A, biapenem |
| CphA metallo-beta-lactamasevariant D120A | Aeromonas hydrophila | imipenem | 0.024 | 0.33 | 0.073 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant D120A, imipenem |
| CphA metallo-beta-lactamasevariant D120A | Aeromonas hydrophila | nitrocefin | 0.0024 | 0.026 | 0.092 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant D120A, nitrocefin |
| CphA metallo-beta-lactamasevariant D120T | Aeromonas hydrophila | imipenem | 0.025 | 0.21 | 0.12 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant D120T, imipenem |
| CphA metallo-beta-lactamasevariant D120T | Aeromonas hydrophila | nitrocefin | 2 × 10⁻⁴ | 0.05 | 0.004 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant D120T, nitrocefin |
| CphA metallo-beta-lactamasevariant H118A | Aeromonas hydrophila | benzylpenicillin | 0.007 | 0.21 | 0.033 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant H118A, benzylpenicillin |
| CphA metallo-beta-lactamasevariant H118A | Aeromonas hydrophila | biapenem | 0.1 | 0.73 | 0.15 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant H118A, biapenem |
| CphA metallo-beta-lactamasevariant H118A | Aeromonas hydrophila | cephaloridine | — | — | 0.0022 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant H118A, cephaloridine |
| CphA metallo-beta-lactamasevariant H118A | Aeromonas hydrophila | imipenem | 2.4 | 1.15 | 2.1 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant H118A, imipenem |
| CphA metallo-beta-lactamasevariant H118A | Aeromonas hydrophila | nitrocefin | 0.005 | 0.03 | 0.17 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant H118A, nitrocefin |
| CphA metallo-beta-lactamasevariant H196A | Aeromonas hydrophila | benzylpenicillin | — | — | 0.02 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant H196A, benzylpenicillin |
| CphA metallo-beta-lactamasevariant H196A | Aeromonas hydrophila | cephaloridine | — | — | 0.024 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant H196A, cephaloridine |
| CphA metallo-beta-lactamasevariant H196A | Aeromonas hydrophila | imipenem | 16 | 4.3 | 3.7 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant H196A, imipenem |
| CphA metallo-beta-lactamasevariant H196A | Aeromonas hydrophila | nitrocefin | 0.006 | 0.015 | 0.4 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant H196A, nitrocefin |
| CphA metallo-beta-lactamasevariant H263A | Aeromonas hydrophila | biapenem | — | — | 0.003 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant H263A, biapenem |
| CphA metallo-beta-lactamasevariant H263A | Aeromonas hydrophila | imipenem | 0.02 | 1.7 | 0.012 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant H263A, imipenem |
| CphA metallo-beta-lactamasevariant H263A | Aeromonas hydrophila | nitrocefin | 0.003 | 0.015 | 0.2 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant H263A, nitrocefin |
| CphA metallo-beta-lactamasevariant K224Q | Aeromonas hydrophila | benzylpenicillin | — | — | 0.006 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant K224Q, benzylpenicillin |
| CphA metallo-beta-lactamasevariant K224Q | Aeromonas hydrophila | biapenem | — | — | 40 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant K224Q, biapenem |
| CphA metallo-beta-lactamasevariant K224Q | Aeromonas hydrophila | cefotaxime | 0.001 | 0.7 | 0.0014 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant K224Q, cefotaxime |
| CphA metallo-beta-lactamasevariant K224Q | Aeromonas hydrophila | cephaloridine | 0.024 | 1 | 0.024 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant K224Q, cephaloridine |
| CphA metallo-beta-lactamasevariant K224Q | Aeromonas hydrophila | imipenem | — | — | 330 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant K224Q, imipenem |
| CphA metallo-beta-lactamasevariant K224Q | Aeromonas hydrophila | nitrocefin | 0.007 | 0.135 | 0.052 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant K224Q, nitrocefin |
| CphA metallo-beta-lactamasevariant K226Q | Aeromonas hydrophila | benzylpenicillin | 0.004 | 0.8 | 0.005 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant K226Q, benzylpenicillin |
| CphA metallo-beta-lactamasevariant K226Q | Aeromonas hydrophila | biapenem | 1 × 10³ | 0.84 | 1.2 × 10³ | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant K226Q, biapenem |
| CphA metallo-beta-lactamasevariant K226Q | Aeromonas hydrophila | cefotaxime | 6 × 10⁻⁴ | 0.14 | 0.0043 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant K226Q, cefotaxime |
| CphA metallo-beta-lactamasevariant K226Q | Aeromonas hydrophila | cephaloridine | 0.006 | 1.7 | 0.0035 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant K226Q, cephaloridine |
| CphA metallo-beta-lactamasevariant K226Q | Aeromonas hydrophila | imipenem | 1 × 10³ | 0.38 | 2.6 × 10³ | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant K226Q, imipenem |
| CphA metallo-beta-lactamasevariant K226Q | Aeromonas hydrophila | nitrocefin | 0.01 | 0.85 | 0.012 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant K226Q, nitrocefin |
| CphA metallo-beta-lactamasevariant N116H/N220G/K224Q | Aeromonas hydrophila | benzylpenicillin | 0.13 | 0.92 | 0.14 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant N116H/N220G/K224Q, benzylpenicillin |
| CphA metallo-beta-lactamasevariant N116H/N220G/K224Q | Aeromonas hydrophila | biapenem | 0.26 | 0.39 | 0.67 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant N116H/N220G/K224Q, biapenem |
| CphA metallo-beta-lactamasevariant N116H/N220G/K224Q | Aeromonas hydrophila | cefotaxime | 0.015 | 0.434 | 0.034 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant N116H/N220G/K224Q, cefotaxime |
| CphA metallo-beta-lactamasevariant N116H/N220G/K224Q | Aeromonas hydrophila | cephaloridine | 0.03 | 0.053 | 0.57 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant N116H/N220G/K224Q, cephaloridine |
| CphA metallo-beta-lactamasevariant N116H/N220G/K224Q | Aeromonas hydrophila | imipenem | 0.9 | 0.3 | 3 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant N116H/N220G/K224Q, imipenem |
| CphA metallo-beta-lactamasevariant N116H/N220G/K224Q | Aeromonas hydrophila | nitrocefin | 0.2 | 0.032 | 6 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant N116H/N220G/K224Q, nitrocefin |
| CphA metallo-beta-lactamasevariant T157A | Aeromonas hydrophila | benzylpenicillin | 1 | 0.55 | 1.95 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant T157A, benzylpenicillin |
| CphA metallo-beta-lactamasevariant T157A | Aeromonas hydrophila | biapenem | — | — | 87 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant T157A, biapenem |
| CphA metallo-beta-lactamasevariant T157A | Aeromonas hydrophila | cefotaxime | 0.023 | 0.45 | 0.05 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant T157A, cefotaxime |
| CphA metallo-beta-lactamasevariant T157A | Aeromonas hydrophila | imipenem | 1.68 × 10³ | 0.57 | 3 × 10³ | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant T157A, imipenem |
| CphA metallo-beta-lactamasevariant T157A | Aeromonas hydrophila | nitrocefin | 0.015 | 0.11 | 0.14 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant T157A, nitrocefin |
| CphA metallo-beta-lactamasevariant V67A | Aeromonas hydrophila | benzylpenicillin | 0.004 | 1 | 0.004 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant V67A, benzylpenicillin |
| CphA metallo-beta-lactamasevariant V67A | Aeromonas hydrophila | biapenem | 100 | 0.1 | 1 × 10³ | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant V67A, biapenem |
| CphA metallo-beta-lactamasevariant V67A | Aeromonas hydrophila | imipenem | 1 × 10³ | 0.22 | 4.5 × 10³ | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant V67A, imipenem |
| CphA metallo-beta-lactamasevariant V67A | Aeromonas hydrophila | nitrocefin | 0.005 | 0.026 | 0.18 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant V67A, nitrocefin |
| CphA metallo-beta-lactamasevariant V67D | Aeromonas hydrophila | benzylpenicillin | — | — | 0.006 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant V67D, benzylpenicillin |
| CphA metallo-beta-lactamasevariant V67D | Aeromonas hydrophila | biapenem | — | — | 66 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant V67D, biapenem |
| CphA metallo-beta-lactamasevariant V67D | Aeromonas hydrophila | imipenem | 1.28 × 10³ | 0.31 | 4 × 10³ | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant V67D, imipenem |
| CphA metallo-beta-lactamasevariant V67D | Aeromonas hydrophila | nitrocefin | 0.004 | 0.036 | 0.11 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant V67D, nitrocefin |
| CphA metallo-beta-lactamasevariant V67I | Aeromonas hydrophila | benzylpenicillin | 0.08 | 0.73 | 0.11 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant V67I, benzylpenicillin |
| CphA metallo-beta-lactamasevariant V67I | Aeromonas hydrophila | biapenem | — | — | 33 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant V67I, biapenem |
| CphA metallo-beta-lactamasevariant V67I | Aeromonas hydrophila | imipenem | 1.77 × 10³ | 0.18 | 9 × 10³ | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant V67I, imipenem |
| CphA metallo-beta-lactamasevariant V67I | Aeromonas hydrophila | nitrocefin | 0.004 | 0.05 | 0.08 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant V67I, nitrocefin |
| CphA metallo-beta-lactamasevariant V67LFKHV | Aeromonas hydrophila | biapenem | — | — | 1.6 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant V67LFKHV, biapenem |
| CphA metallo-beta-lactamasevariant V67LFKHV | Aeromonas hydrophila | imipenem | — | — | 20 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant V67LFKHV, imipenem |
| CphA metallo-beta-lactamasevariant V67LFKHV | Aeromonas hydrophila | nitrocefin | — | — | 0.003 | pH 6.5, 30 °C | Open record: CphA metallo-beta-lactamase variant V67LFKHV, nitrocefin |