Values CatLog read from this paper
Structure and mechanism of the rebeccamycin sugar 4'-O-methyltransferase RebM
PMID 18502766doi:10.1074/jbc.m800503200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 20 records (30 values) from this paper: RebM (rebeccamycin sugar 4'-O-methyltransferase) from Lechevalieria aerocolonigenes.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 10 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| RebM (rebeccamycin sugar 4'-O-methyltransferase) | Lechevalieria aerocolonigenes | 4'-O-demethylrebeccamycin | 0.047 | 0.0355 | 1.3 calc. | pH 8, 25 °C | Open record: RebM (rebeccamycin sugar 4'-O-methyltransferase), 4'-O-demethylrebeccamycin |
| RebM (rebeccamycin sugar 4'-O-methyltransferase) | Lechevalieria aerocolonigenes | S-adenosylmethionine | — | 0.012 | — | pH 8, 25 °C | Open record: RebM (rebeccamycin sugar 4'-O-methyltransferase), S-adenosylmethionine |
| RebM (rebeccamycin sugar 4'-O-methyltransferase)variant C70A | Lechevalieria aerocolonigenes | 4'-O-demethylrebeccamycin | 0.048 | 0.0225 | 2.1 calc. | pH 8, 25 °C | Open record: RebM (rebeccamycin sugar 4'-O-methyltransferase) variant C70A, 4'-O-demethylrebeccamycin |
| RebM (rebeccamycin sugar 4'-O-methyltransferase)variant C70A | Lechevalieria aerocolonigenes | S-adenosylmethionine | — | 0.017 | — | pH 8, 25 °C | Open record: RebM (rebeccamycin sugar 4'-O-methyltransferase) variant C70A, S-adenosylmethionine |
| RebM (rebeccamycin sugar 4'-O-methyltransferase)variant C70S | Lechevalieria aerocolonigenes | 4'-O-demethylrebeccamycin | 0.072 | 0.017 | 4.2 calc. | pH 8, 25 °C | Open record: RebM (rebeccamycin sugar 4'-O-methyltransferase) variant C70S, 4'-O-demethylrebeccamycin |
| RebM (rebeccamycin sugar 4'-O-methyltransferase)variant C70S | Lechevalieria aerocolonigenes | S-adenosylmethionine | — | 0.0345 | — | pH 8, 25 °C | Open record: RebM (rebeccamycin sugar 4'-O-methyltransferase) variant C70S, S-adenosylmethionine |
| RebM (rebeccamycin sugar 4'-O-methyltransferase)variant D166A | Lechevalieria aerocolonigenes | 4'-O-demethylrebeccamycin | 0.005 | 0.03199 | 0.16 calc. | pH 8, 25 °C | Open record: RebM (rebeccamycin sugar 4'-O-methyltransferase) variant D166A, 4'-O-demethylrebeccamycin |
| RebM (rebeccamycin sugar 4'-O-methyltransferase)variant D166A | Lechevalieria aerocolonigenes | S-adenosylmethionine | — | 0.162 | — | pH 8, 25 °C | Open record: RebM (rebeccamycin sugar 4'-O-methyltransferase) variant D166A, S-adenosylmethionine |
| RebM (rebeccamycin sugar 4'-O-methyltransferase)variant H140A | Lechevalieria aerocolonigenes | 4'-O-demethylrebeccamycin | 0.0023 | 0.0112 | 0.21 calc. | pH 8, 25 °C | Open record: RebM (rebeccamycin sugar 4'-O-methyltransferase) variant H140A, 4'-O-demethylrebeccamycin |
| RebM (rebeccamycin sugar 4'-O-methyltransferase)variant H140A | Lechevalieria aerocolonigenes | S-adenosylmethionine | — | 0.0311 | — | pH 8, 25 °C | Open record: RebM (rebeccamycin sugar 4'-O-methyltransferase) variant H140A, S-adenosylmethionine |
| RebM (rebeccamycin sugar 4'-O-methyltransferase)variant H141A | Lechevalieria aerocolonigenes | 4'-O-demethylrebeccamycin | 0.01 | 0.0798 | 0.13 calc. | pH 8, 25 °C | Open record: RebM (rebeccamycin sugar 4'-O-methyltransferase) variant H141A, 4'-O-demethylrebeccamycin |
| RebM (rebeccamycin sugar 4'-O-methyltransferase)variant H141A | Lechevalieria aerocolonigenes | S-adenosylmethionine | — | 0.0943 | — | pH 8, 25 °C | Open record: RebM (rebeccamycin sugar 4'-O-methyltransferase) variant H141A, S-adenosylmethionine |
| RebM (rebeccamycin sugar 4'-O-methyltransferase)variant L136V | Lechevalieria aerocolonigenes | 4'-O-demethylrebeccamycin | 0.0253 | 0.0215 | 1.18 calc. | pH 8, 25 °C | Open record: RebM (rebeccamycin sugar 4'-O-methyltransferase) variant L136V, 4'-O-demethylrebeccamycin |
| RebM (rebeccamycin sugar 4'-O-methyltransferase)variant L136V | Lechevalieria aerocolonigenes | S-adenosylmethionine | — | 0.056 | — | pH 8, 25 °C | Open record: RebM (rebeccamycin sugar 4'-O-methyltransferase) variant L136V, S-adenosylmethionine |
| RebM (rebeccamycin sugar 4'-O-methyltransferase)variant P75S | Lechevalieria aerocolonigenes | 4'-O-demethylrebeccamycin | 0.0063 | 0.0535 | 0.12 calc. | pH 8, 25 °C | Open record: RebM (rebeccamycin sugar 4'-O-methyltransferase) variant P75S, 4'-O-demethylrebeccamycin |
| RebM (rebeccamycin sugar 4'-O-methyltransferase)variant P75S | Lechevalieria aerocolonigenes | S-adenosylmethionine | — | 0.162 | — | pH 8, 25 °C | Open record: RebM (rebeccamycin sugar 4'-O-methyltransferase) variant P75S, S-adenosylmethionine |
| RebM (rebeccamycin sugar 4'-O-methyltransferase)variant S138A | Lechevalieria aerocolonigenes | 4'-O-demethylrebeccamycin | 0.0232 | 0.021 | 1.1 calc. | pH 8, 25 °C | Open record: RebM (rebeccamycin sugar 4'-O-methyltransferase) variant S138A, 4'-O-demethylrebeccamycin |
| RebM (rebeccamycin sugar 4'-O-methyltransferase)variant S138A | Lechevalieria aerocolonigenes | S-adenosylmethionine | — | 0.018 | — | pH 8, 25 °C | Open record: RebM (rebeccamycin sugar 4'-O-methyltransferase) variant S138A, S-adenosylmethionine |
| RebM (rebeccamycin sugar 4'-O-methyltransferase)variant W134Y | Lechevalieria aerocolonigenes | 4'-O-demethylrebeccamycin | 0.0167 | 0.016 | 1.0 calc. | pH 8, 25 °C | Open record: RebM (rebeccamycin sugar 4'-O-methyltransferase) variant W134Y, 4'-O-demethylrebeccamycin |
| RebM (rebeccamycin sugar 4'-O-methyltransferase)variant W134Y | Lechevalieria aerocolonigenes | S-adenosylmethionine | — | 0.032 | — | pH 8, 25 °C | Open record: RebM (rebeccamycin sugar 4'-O-methyltransferase) variant W134Y, S-adenosylmethionine |