Values CatLog read from this paper
A new member of the alkaline phosphatase superfamily with a formylglycine nucleophile: structural and kinetic characterisation of a phosphonate monoester hydrolase/phosphodiesterase from Rhizobium…
PMID 18793651doi:10.1016/j.jmb.2008.08.072Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 21 records (55 values) from this paper: phosphonate monoester hydrolase/phosphodiesterase (RlPMH) from Rhizobium leguminosarum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| phosphonate monoester hydrolase/phosphodiesterase (RlPMH) | Rhizobium leguminosarum | 4-nitrophenyl ethyl phosphate | 9.5 | 2.3 | 4.1 | pH 7.5, 30 °C | Open record: phosphonate monoester hydrolase/phosphodiesterase (RlPMH), 4-nitrophenyl ethyl phosphate |
| phosphonate monoester hydrolase/phosphodiesterase (RlPMH) | Rhizobium leguminosarum | 4-nitrophenyl phenyl phosphonate4-nitrophenol release at 400 nm; initial rates; Table 1 | 16 | 3 | 5.3 | pH 7.5, 30 °C | Open record: phosphonate monoester hydrolase/phosphodiesterase (RlPMH), 4-nitrophenyl phenyl phosphonate |
| phosphonate monoester hydrolase/phosphodiesterase (RlPMH) | Rhizobium leguminosarum | 4-nitrophenyl phenyl phosphonate1x SID buffer; in the text | 53 | — | — | pH 7.5, 30 °C | Open record: phosphonate monoester hydrolase/phosphodiesterase (RlPMH), 4-nitrophenyl phenyl phosphonate |
| phosphonate monoester hydrolase/phosphodiesterase (RlPMH) | Rhizobium leguminosarum | 4-nitrophenyl phenyl phosphonate1x SID buffer; in the text | 12 | — | — | pH 7.5, 30 °C | Open record: phosphonate monoester hydrolase/phosphodiesterase (RlPMH), 4-nitrophenyl phenyl phosphonate |
| phosphonate monoester hydrolase/phosphodiesterase (RlPMH) | Rhizobium leguminosarum | 4-nitrophenyl sulfate | — | — | 0.0031 | Open record: phosphonate monoester hydrolase/phosphodiesterase (RlPMH), 4-nitrophenyl sulfate | |
| phosphonate monoester hydrolase/phosphodiesterase (RlPMH)variant C57A | Rhizobium leguminosarum | 4-nitrophenyl ethyl phosphate | 0.0047 | 2.9 | 0.0016 | pH 7.5, 30 °C | Open record: phosphonate monoester hydrolase/phosphodiesterase (RlPMH) variant C57A, 4-nitrophenyl ethyl phosphate |
| phosphonate monoester hydrolase/phosphodiesterase (RlPMH)variant C57A | Rhizobium leguminosarum | 4-nitrophenyl phenyl phosphonate | 0.0088 | 2.8 | 0.0032 | pH 7.5, 30 °C | Open record: phosphonate monoester hydrolase/phosphodiesterase (RlPMH) variant C57A, 4-nitrophenyl phenyl phosphonate |
| phosphonate monoester hydrolase/phosphodiesterase (RlPMH)variant C57S | Rhizobium leguminosarum | 4-nitrophenyl ethyl phosphate | 1.6 | 1.7 | 0.91 | pH 7.5, 30 °C | Open record: phosphonate monoester hydrolase/phosphodiesterase (RlPMH) variant C57S, 4-nitrophenyl ethyl phosphate |
| phosphonate monoester hydrolase/phosphodiesterase (RlPMH)variant C57S | Rhizobium leguminosarum | 4-nitrophenyl phenyl phosphonate | 3.3 | 3.8 | 0.87 | pH 7.5, 30 °C | Open record: phosphonate monoester hydrolase/phosphodiesterase (RlPMH) variant C57S, 4-nitrophenyl phenyl phosphonate |
| phosphonate monoester hydrolase/phosphodiesterase (RlPMH)variant H218A | Rhizobium leguminosarum | 4-nitrophenyl ethyl phosphate | 0.3 | 57 | 0.0052 | pH 7.5, 30 °C | Open record: phosphonate monoester hydrolase/phosphodiesterase (RlPMH) variant H218A, 4-nitrophenyl ethyl phosphate |
| phosphonate monoester hydrolase/phosphodiesterase (RlPMH)variant H218A | Rhizobium leguminosarum | 4-nitrophenyl phenyl phosphonate | 1 | 15 | 0.066 | pH 7.5, 30 °C | Open record: phosphonate monoester hydrolase/phosphodiesterase (RlPMH) variant H218A, 4-nitrophenyl phenyl phosphonate |
| phosphonate monoester hydrolase/phosphodiesterase (RlPMH)variant K337A | Rhizobium leguminosarum | 4-nitrophenyl ethyl phosphate | 2.9 | 73 | 0.04 | pH 7.5, 30 °C | Open record: phosphonate monoester hydrolase/phosphodiesterase (RlPMH) variant K337A, 4-nitrophenyl ethyl phosphate |
| phosphonate monoester hydrolase/phosphodiesterase (RlPMH)variant K337A | Rhizobium leguminosarum | 4-nitrophenyl phenyl phosphonate | 12 | 14 | 0.85 | pH 7.5, 30 °C | Open record: phosphonate monoester hydrolase/phosphodiesterase (RlPMH) variant K337A, 4-nitrophenyl phenyl phosphonate |
| phosphonate monoester hydrolase/phosphodiesterase (RlPMH)variant N78A | Rhizobium leguminosarum | 4-nitrophenyl ethyl phosphate | 1.1 | 45 | 0.025 | pH 7.5, 30 °C | Open record: phosphonate monoester hydrolase/phosphodiesterase (RlPMH) variant N78A, 4-nitrophenyl ethyl phosphate |
| phosphonate monoester hydrolase/phosphodiesterase (RlPMH)variant N78A | Rhizobium leguminosarum | 4-nitrophenyl phenyl phosphonate | 1.5 | 4 | 0.37 | pH 7.5, 30 °C | Open record: phosphonate monoester hydrolase/phosphodiesterase (RlPMH) variant N78A, 4-nitrophenyl phenyl phosphonate |
| phosphonate monoester hydrolase/phosphodiesterase (RlPMH)variant Q13A | Rhizobium leguminosarum | 4-nitrophenyl ethyl phosphate | 18 | 50 | 0.36 | pH 7.5, 30 °C | Open record: phosphonate monoester hydrolase/phosphodiesterase (RlPMH) variant Q13A, 4-nitrophenyl ethyl phosphate |
| phosphonate monoester hydrolase/phosphodiesterase (RlPMH)variant Q13A | Rhizobium leguminosarum | 4-nitrophenyl phenyl phosphonate | 26 | 43 | 0.6 | pH 7.5, 30 °C | Open record: phosphonate monoester hydrolase/phosphodiesterase (RlPMH) variant Q13A, 4-nitrophenyl phenyl phosphonate |
| phosphonate monoester hydrolase/phosphodiesterase (RlPMH)variant T107A | Rhizobium leguminosarum | 4-nitrophenyl ethyl phosphate | 0.57 | 4.8 | 0.12 | pH 7.5, 30 °C | Open record: phosphonate monoester hydrolase/phosphodiesterase (RlPMH) variant T107A, 4-nitrophenyl ethyl phosphate |
| phosphonate monoester hydrolase/phosphodiesterase (RlPMH)variant T107A | Rhizobium leguminosarum | 4-nitrophenyl phenyl phosphonate | 2.4 | 0.31 | 7.7 | pH 7.5, 30 °C | Open record: phosphonate monoester hydrolase/phosphodiesterase (RlPMH) variant T107A, 4-nitrophenyl phenyl phosphonate |
| phosphonate monoester hydrolase/phosphodiesterase (RlPMH)variant Y105A | Rhizobium leguminosarum | 4-nitrophenyl ethyl phosphate | — | — | 0.0046 | pH 7.5, 30 °C | Open record: phosphonate monoester hydrolase/phosphodiesterase (RlPMH) variant Y105A, 4-nitrophenyl ethyl phosphate |
| phosphonate monoester hydrolase/phosphodiesterase (RlPMH)variant Y105A | Rhizobium leguminosarum | 4-nitrophenyl phenyl phosphonate | 2.7 | 28 | 0.099 | pH 7.5, 30 °C | Open record: phosphonate monoester hydrolase/phosphodiesterase (RlPMH) variant Y105A, 4-nitrophenyl phenyl phosphonate |