Values CatLog read from this paper
Using a molecular model and kinetic experiments in the presence of divalent cations to study the active site and catalysis of Pseudomonas aeruginosa phosphorylcholine phosphatase
PMID 18801468doi:10.1016/j.bbapap.2008.08.014Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 14 records (42 values) from this paper: phosphorylcholine phosphatase (PchP) from Pseudomonas aeruginosa.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| phosphorylcholine phosphatase (PchP) | Pseudomonas aeruginosa | 4-nitrophenyl phosphateactivator Mg2+ varied 0.2-8.0 mM; p-NPP 0.5-10 mM | 1.6 × 10⁶ | 3.2 | 5.1 × 10⁵ | pH 5, 37 °C | Open record: phosphorylcholine phosphatase (PchP), 4-nitrophenyl phosphate |
| phosphorylcholine phosphatase (PchP) | Pseudomonas aeruginosa | 4-nitrophenyl phosphateactivator Zn2+ varied 2.5-100 µM; p-NPP 0.5-10 mM | 5.5 × 10⁶ | 3.5 | 1.6 × 10⁶ | pH 5, 37 °C | Open record: phosphorylcholine phosphatase (PchP), 4-nitrophenyl phosphate |
| phosphorylcholine phosphatase (PchP) | Pseudomonas aeruginosa | 4-nitrophenyl phosphateactivator Cu2+ varied 2.5-100 µM; p-NPP 5-80 mM | 7.9 × 10⁶ | 12.2 | 6.5 × 10⁵ | pH 5, 37 °C | Open record: phosphorylcholine phosphatase (PchP), 4-nitrophenyl phosphate |
| phosphorylcholine phosphatase (PchP)variant D262E | Pseudomonas aeruginosa | 4-nitrophenyl phosphateactivator Mg2+ varied 0.2-8.0 mM; p-NPP 0.5-10 mM | 1.1 × 10³ | 7.6 | 150 | pH 5, 37 °C | Open record: phosphorylcholine phosphatase (PchP) variant D262E, 4-nitrophenyl phosphate |
| phosphorylcholine phosphatase (PchP)variant D262E | Pseudomonas aeruginosa | 4-nitrophenyl phosphateactivator Zn2+ varied 2.5-100 µM; p-NPP 0.5-10 mM | 1.4 × 10³ | 1.4 | 1 × 10³ | pH 5, 37 °C | Open record: phosphorylcholine phosphatase (PchP) variant D262E, 4-nitrophenyl phosphate |
| phosphorylcholine phosphatase (PchP)variant D262E | Pseudomonas aeruginosa | 4-nitrophenyl phosphateactivator Cu2+ varied 2.5-100 µM; p-NPP 5-80 mM | 1.9 × 10⁴ | 22.2 | 870 | pH 5, 37 °C | Open record: phosphorylcholine phosphatase (PchP) variant D262E, 4-nitrophenyl phosphate |
| phosphorylcholine phosphatase (PchP)variant D267E | Pseudomonas aeruginosa | 4-nitrophenyl phosphateactivator Mg2+ varied 0.2-8.0 mM | 0.2 | 11.5 | 14.9 | pH 5, 37 °C | Open record: phosphorylcholine phosphatase (PchP) variant D267E, 4-nitrophenyl phosphate |
| phosphorylcholine phosphatase (PchP)variant D267E | Pseudomonas aeruginosa | 4-nitrophenyl phosphateactivator Zn2+ varied 2.5-100 µM | 0.7 | 5.1 | 13.7 | pH 5, 37 °C | Open record: phosphorylcholine phosphatase (PchP) variant D267E, 4-nitrophenyl phosphate |
| phosphorylcholine phosphatase (PchP)variant S166T | Pseudomonas aeruginosa | 4-nitrophenyl phosphateactivator Mg2+ varied 0.2-8.0 mM; p-NPP 0.5-10 mM | 2.1 × 10⁴ | 2.5 | 8.4 × 10³ | pH 5, 37 °C | Open record: phosphorylcholine phosphatase (PchP) variant S166T, 4-nitrophenyl phosphate |
| phosphorylcholine phosphatase (PchP)variant S166T | Pseudomonas aeruginosa | 4-nitrophenyl phosphateactivator Zn2+ varied 2.5-100 µM; p-NPP 0.5-10 mM | 2.9 × 10⁴ | 5.4 | 5.4 × 10³ | pH 5, 37 °C | Open record: phosphorylcholine phosphatase (PchP) variant S166T, 4-nitrophenyl phosphate |
| phosphorylcholine phosphatase (PchP)variant S166T | Pseudomonas aeruginosa | 4-nitrophenyl phosphateactivator Cu2+ varied 2.5-100 µM; p-NPP 5-80 mM | 2.2 × 10⁴ | 18.7 | 1.2 × 10³ | pH 5, 37 °C | Open record: phosphorylcholine phosphatase (PchP) variant S166T, 4-nitrophenyl phosphate |
| phosphorylcholine phosphatase (PchP)variant T35S | Pseudomonas aeruginosa | 4-nitrophenyl phosphateactivator Mg2+ varied 0.2-8.0 mM; p-NPP 0.5-10 mM | 1.2 × 10⁵ | 4.3 | 2.8 × 10⁴ | pH 5, 37 °C | Open record: phosphorylcholine phosphatase (PchP) variant T35S, 4-nitrophenyl phosphate |
| phosphorylcholine phosphatase (PchP)variant T35S | Pseudomonas aeruginosa | 4-nitrophenyl phosphateactivator Zn2+ varied 2.5-100 µM; p-NPP 0.5-10 mM | 6.5 × 10⁵ | 2.6 | 2.5 × 10⁵ | pH 5, 37 °C | Open record: phosphorylcholine phosphatase (PchP) variant T35S, 4-nitrophenyl phosphate |
| phosphorylcholine phosphatase (PchP)variant T35S | Pseudomonas aeruginosa | 4-nitrophenyl phosphateactivator Cu2+ varied 2.5-100 µM; p-NPP 5-80 mM | 2.5 × 10⁶ | 13.1 | 1.9 × 10⁵ | pH 5, 37 °C | Open record: phosphorylcholine phosphatase (PchP) variant T35S, 4-nitrophenyl phosphate |