Values CatLog read from this paper
Characterization of a phenylacetate-CoA ligase from Penicillium chrysogenum
PMID 18834333doi:10.1042/bj20081257Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 75 records (169 values) from this paper: phenylacetate-CoA ligase (PCL) from Penicillium chrysogenum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 46 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | 3,4-Methylenedioxycinnamic acid | 11.2 | 0.54 | 21 | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), 3,4-Methylenedioxycinnamic acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | 3-Ethoxycinnamic acid | 12.1 | 0.82 | 15 | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), 3-Ethoxycinnamic acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | 3-Methoxycinnamic acid | 11.6 | 0.84 | 14 | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), 3-Methoxycinnamic acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | 3-Phenylpropionic acid | 22.7 | 2.1 | 10.8 | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), 3-Phenylpropionic acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | 4-hydroxyphenylacetic acid | 0.08 | 5.7 | 0.014 | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), 4-hydroxyphenylacetic acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | ATPspectrophotometric assay; 1.5 mM trans-cinnamic acid | 42.9 | — | — | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), ATP |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | ATPAMP formation measured by reverse-phase HPLC; 60 mM PAA | 1.7 | 0.65 | 2.6 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), ATP |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | Butyric acid | 9.7 | 24.4 | 0.4 | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), Butyric acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | Caffeic acid | — | — | 0.02 | pH 7.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), Caffeic acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | Capric acid | 8.2 | 0.002 | 4.1 × 10³ | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), Capric acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | Caproic acid | 15.8 | 0.24 | 66 | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), Caproic acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | Caprylic acid | 11.2 | 0.06 | 190 | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), Caprylic acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | coenzyme Aspectrophotometric assay; 1.5 mM trans-cinnamic acid | 60.5 | 0.94 | 64 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), coenzyme A |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | coenzyme AAMP formation measured by reverse-phase HPLC; 60 mM PAA | 1.5 | 0.12 | 12 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), coenzyme A |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | Enanthic acid | 12.3 | 0.05 | 250 | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), Enanthic acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | Ferulic acid | — | — | 0.07 | pH 7.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), Ferulic acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | Lauric acid | 8.5 | 4 × 10⁻⁴ | 2.1 × 10⁴ | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), Lauric acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | Myristic acid | 2.3 | — | — | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), Myristic acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | phenoxyacetic acidapparent kcat/Km | 15.6 | 2 | 7.8 | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), phenoxyacetic acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | phenoxyacetic acid | 12 | 2.1 | 5.7 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), phenoxyacetic acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | phenylacetic acidapparent kcat/Km | 1.4 | 6.1 | 0.23 | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), phenylacetic acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | phenylacetic acid | 1.9 | 5.2 | 0.37 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), phenylacetic acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | Propionic acid | — | — | 0.013 | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), Propionic acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | trans-3-Hexenoic acid | 14.7 | 0.12 | 120 | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), trans-3-Hexenoic acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | trans-4-Chlorocinnamic acid | 29.1 | 0.16 | 180 | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), trans-4-Chlorocinnamic acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | trans-4-Coumaric acid | 3.4 | 0.47 | 7.2 | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), trans-4-Coumaric acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | trans-4-Fluorocinnamic acid | 35.5 | 0.084 | 420 | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), trans-4-Fluorocinnamic acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | trans-4-Isopropylcinnamic acid | 9.6 | 0.07 | 140 | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), trans-4-Isopropylcinnamic acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | trans-4-Methoxycinnamic acid | 23.9 | 0.18 | 130 | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), trans-4-Methoxycinnamic acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | trans-4-Methylcinnamic acid | 21 | 0.32 | 66 | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), trans-4-Methylcinnamic acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | trans-4-Nitrocinnamic acid | 2.8 | 0.51 | 5.5 | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), trans-4-Nitrocinnamic acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | trans-4-Trifluoromethylcinnamic acid | 11.3 | 0.19 | 60 | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), trans-4-Trifluoromethylcinnamic acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | trans-Cinnamic acid | 43.5 | 0.14 | 310 | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), trans-Cinnamic acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | trans-cinnamic acid | 41.2 | 0.19 | 220 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), trans-cinnamic acid |
| phenylacetate-CoA ligase (PCL) | Penicillium chrysogenum | Valeric acid | 18.5 | 0.7 | 260 | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL), Valeric acid |
| phenylacetate-CoA ligase (PCL)variant F307A | Penicillium chrysogenum | Caproic acid | 5.3 | 0.24 | 22 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant F307A, Caproic acid |
| phenylacetate-CoA ligase (PCL)variant F307A | Penicillium chrysogenum | phenoxyacetic acid | 0.53 | 5.3 | 0.10 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant F307A, phenoxyacetic acid |
| phenylacetate-CoA ligase (PCL)variant F307A | Penicillium chrysogenum | phenylacetic acid | 0.017 | 6.8 | 0.0025 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant F307A, phenylacetic acid |
| phenylacetate-CoA ligase (PCL)variant F307A | Penicillium chrysogenum | trans-4-coumaric acid | 0.11 | 1.1 | 0.10 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant F307A, trans-4-coumaric acid |
| phenylacetate-CoA ligase (PCL)variant F307A | Penicillium chrysogenum | trans-cinnamic acid | 4.5 | 1 | 4.5 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant F307A, trans-cinnamic acid |
| phenylacetate-CoA ligase (PCL)variant F335A | Penicillium chrysogenum | Caproic acid | 11.3 | 0.8 | 14 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant F335A, Caproic acid |
| phenylacetate-CoA ligase (PCL)variant F335A | Penicillium chrysogenum | phenoxyacetic acid | 0.91 | 10.3 | 0.088 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant F335A, phenoxyacetic acid |
| phenylacetate-CoA ligase (PCL)variant F335A | Penicillium chrysogenum | phenylacetic acid | 0.04 | 8 | 0.0050 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant F335A, phenylacetic acid |
| phenylacetate-CoA ligase (PCL)variant F335A | Penicillium chrysogenum | trans-4-coumaric acid | 0.8 | 0.17 | 4.7 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant F335A, trans-4-coumaric acid |
| phenylacetate-CoA ligase (PCL)variant F335A | Penicillium chrysogenum | trans-cinnamic acid | 12.1 | 2.5 | 4.8 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant F335A, trans-cinnamic acid |
| phenylacetate-CoA ligase (PCL)variant H265A | Penicillium chrysogenum | Caproic acid | 4.4 | 1.6 | 2.8 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant H265A, Caproic acid |
| phenylacetate-CoA ligase (PCL)variant H265A | Penicillium chrysogenum | phenoxyacetic acid | 0.41 | 4.8 | 0.085 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant H265A, phenoxyacetic acid |
| phenylacetate-CoA ligase (PCL)variant H265A | Penicillium chrysogenum | phenylacetic acid | 0.009 | 8.5 | 0.0011 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant H265A, phenylacetic acid |
| phenylacetate-CoA ligase (PCL)variant H265A | Penicillium chrysogenum | trans-4-coumaric acid | 0.08 | 2.8 | 0.029 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant H265A, trans-4-coumaric acid |
| phenylacetate-CoA ligase (PCL)variant H265A | Penicillium chrysogenum | trans-cinnamic acid | 1.2 | 4 | 0.30 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant H265A, trans-cinnamic acid |
| phenylacetate-CoA ligase (PCL)variant I266A | Penicillium chrysogenum | Caproic acid | 41.8 | 0.3 | 140 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant I266A, Caproic acid |
| phenylacetate-CoA ligase (PCL)variant I266A | Penicillium chrysogenum | phenoxyacetic acid | 1.1 | 3.3 | 0.33 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant I266A, phenoxyacetic acid |
| phenylacetate-CoA ligase (PCL)variant I266A | Penicillium chrysogenum | phenylacetic acid | 0.49 | 5.5 | 0.089 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant I266A, phenylacetic acid |
| phenylacetate-CoA ligase (PCL)variant I266A | Penicillium chrysogenum | trans-4-coumaric acid | 0.6 | 1.8 | 0.33 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant I266A, trans-4-coumaric acid |
| phenylacetate-CoA ligase (PCL)variant I266A | Penicillium chrysogenum | trans-cinnamic acid | 10.4 | 0.8 | 13 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant I266A, trans-cinnamic acid |
| phenylacetate-CoA ligase (PCL)variant T369A | Penicillium chrysogenum | Caproic acid | 47.6 | 0.11 | 430 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant T369A, Caproic acid |
| phenylacetate-CoA ligase (PCL)variant T369A | Penicillium chrysogenum | phenoxyacetic acid | 2.2 | 1.3 | 1.7 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant T369A, phenoxyacetic acid |
| phenylacetate-CoA ligase (PCL)variant T369A | Penicillium chrysogenum | phenylacetic acid | 5.2 | 4.7 | 1.1 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant T369A, phenylacetic acid |
| phenylacetate-CoA ligase (PCL)variant T369A | Penicillium chrysogenum | trans-4-coumaric acid | 3 | 0.47 | 6.4 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant T369A, trans-4-coumaric acid |
| phenylacetate-CoA ligase (PCL)variant T369A | Penicillium chrysogenum | trans-cinnamic acid | 56 | 0.5 | 110 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant T369A, trans-cinnamic acid |
| phenylacetate-CoA ligase (PCL)variant V270A | Penicillium chrysogenum | Caproic acid | 10 | 0.13 | 77 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant V270A, Caproic acid |
| phenylacetate-CoA ligase (PCL)variant V270A | Penicillium chrysogenum | phenoxyacetic acid | 1.5 | 2.4 | 0.62 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant V270A, phenoxyacetic acid |
| phenylacetate-CoA ligase (PCL)variant V270A | Penicillium chrysogenum | phenylacetic acid | 0.68 | 3.9 | 0.17 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant V270A, phenylacetic acid |
| phenylacetate-CoA ligase (PCL)variant V270A | Penicillium chrysogenum | trans-4-coumaric acid | 0.3 | 0.41 | 0.73 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant V270A, trans-4-coumaric acid |
| phenylacetate-CoA ligase (PCL)variant V270A | Penicillium chrysogenum | trans-cinnamic acid | 11.5 | 0.54 | 21 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant V270A, trans-cinnamic acid |
| phenylacetate-CoA ligase (PCL)variant V370A | Penicillium chrysogenum | Caproic acid | 13.9 | 0.15 | 93 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant V370A, Caproic acid |
| phenylacetate-CoA ligase (PCL)variant V370A | Penicillium chrysogenum | phenoxyacetic acid | 4.2 | 4 | 1.1 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant V370A, phenoxyacetic acid |
| phenylacetate-CoA ligase (PCL)variant V370A | Penicillium chrysogenum | phenylacetic acid | 0.14 | 7.6 | 0.018 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant V370A, phenylacetic acid |
| phenylacetate-CoA ligase (PCL)variant V370A | Penicillium chrysogenum | trans-4-coumaric acid | 1.3 | 0.14 | 9.3 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant V370A, trans-4-coumaric acid |
| phenylacetate-CoA ligase (PCL)variant V370A | Penicillium chrysogenum | trans-cinnamic acid | 24.6 | 0.25 | 98 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant V370A, trans-cinnamic acid |
| phenylacetate-CoA ligase (PCL)variant Y267A | Penicillium chrysogenum | Caproic acid | 0.13 | 0.13 | 1.0 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant Y267A, Caproic acid |
| phenylacetate-CoA ligase (PCL)variant Y267A | Penicillium chrysogenum | phenoxyacetic acid | 0.13 | 10.7 | 0.012 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant Y267A, phenoxyacetic acid |
| phenylacetate-CoA ligase (PCL)variant Y267A | Penicillium chrysogenum | phenylacetic acid | 0.025 | 6.3 | 0.0040 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant Y267A, phenylacetic acid |
| phenylacetate-CoA ligase (PCL)variant Y267A | Penicillium chrysogenum | trans-4-coumaric acid | 0.011 | 0.57 | 0.019 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant Y267A, trans-4-coumaric acid |
| phenylacetate-CoA ligase (PCL)variant Y267A | Penicillium chrysogenum | trans-cinnamic acid | 0.4 | 3 | 0.13 calc. | pH 8.5, 30 °C | Open record: phenylacetate-CoA ligase (PCL) variant Y267A, trans-cinnamic acid |