Values CatLog read from this paper
Functional evaluation of serine 252 of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase
PMID 18996167doi:10.1016/j.biochi.2008.10.005Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (17 values) from this paper: phosphoenolpyruvate carboxykinase from Saccharomyces cerevisiae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 5 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| phosphoenolpyruvate carboxykinase | Saccharomyces cerevisiae | MnATP2− | — | 0.03 | — | pH 7, 30 °C | Open record: phosphoenolpyruvate carboxykinase, MnATP2− |
| phosphoenolpyruvate carboxykinase | Saccharomyces cerevisiae | oxaloacetatePEP-forming direction; 50 mM KCl; 2 mM MnCl2; 0.5 mM OAA | 65 | 0.067 | 970 calc. | pH 7, 30 °C | Open record: phosphoenolpyruvate carboxykinase, oxaloacetate |
| phosphoenolpyruvate carboxykinase | Saccharomyces cerevisiae | oxaloacetatePEP-forming direction; 50 mM KCl; 2 mM MnCl2; 0.5 mM OAA | — | — | 970 | pH 7, 30 °C | Open record: phosphoenolpyruvate carboxykinase, oxaloacetate |
| phosphoenolpyruvate carboxykinase | Saccharomyces cerevisiae | oxaloacetateOAA decarboxylase activity (OAA to pyruvate + CO2); Table 3 | 0.32 | 0.122 | 2.6 calc. | pH 7, 30 °C | Open record: phosphoenolpyruvate carboxykinase, oxaloacetate |
| phosphoenolpyruvate carboxykinasevariant S252A | Saccharomyces cerevisiae | bicarbonate | — | 18 | — | pH 6.6, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant S252A, bicarbonate |
| phosphoenolpyruvate carboxykinasevariant S252A | Saccharomyces cerevisiae | MnADP−PEP carboxylation (OAA-forming) direction; 50 mM KHCO3 | — | 0.132 | — | pH 6.6, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant S252A, MnADP− |
| phosphoenolpyruvate carboxykinasevariant S252A | Saccharomyces cerevisiae | MnADP−pyruvate kinase-like activity (PEP + ADP to pyruvate + ATP) | — | 0.077 | — | pH 6.6, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant S252A, MnADP− |
| phosphoenolpyruvate carboxykinasevariant S252A | Saccharomyces cerevisiae | MnATP2− | — | 0.026 | — | pH 7, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant S252A, MnATP2− |
| phosphoenolpyruvate carboxykinasevariant S252A | Saccharomyces cerevisiae | oxaloacetatePEP-forming direction; 50 mM KCl; 2 mM MnCl2; 0.5 mM OAA | — | — | 6.3 | pH 7, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant S252A, oxaloacetate |
| phosphoenolpyruvate carboxykinasevariant S252A | Saccharomyces cerevisiae | oxaloacetateOAA decarboxylase activity (OAA to pyruvate + CO2) | 1 | 5.2 | 0.19 calc. | pH 7, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant S252A, oxaloacetate |
| phosphoenolpyruvate carboxykinasevariant S252A | Saccharomyces cerevisiae | phosphoenolpyruvatePEP carboxylation (OAA-forming) direction; 50 mM KHCO3 | 0.5 | 0.082 | 6.1 calc. | pH 6.6, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant S252A, phosphoenolpyruvate |
| phosphoenolpyruvate carboxykinasevariant S252A | Saccharomyces cerevisiae | phosphoenolpyruvatepyruvate kinase-like activity (PEP + ADP to pyruvate + ATP) | 0.2 | 0.037 | 5.4 calc. | pH 6.6, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant S252A, phosphoenolpyruvate |