CatLogenzyme kinetics catalog

Values CatLog read from this paper

Tyr235 of human cytosolic phosphoenolpyruvate carboxykinase influences catalysis through an anion-quadrupole interaction with phosphoenolpyruvate carboxylate

PMID 19021757doi:10.1111/j.1742-4658.2008.06702.xOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 38 records (76 values) from this paper: cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK) from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 4.1.1.32

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)Homo sapiensbicarbonate—20.72pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)Homo sapiensguanosine 5'-diphosphate2 mM MgCl2—0.045840pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)Homo sapiensguanosine 5'-diphosphate5 mM MgCl2—0.044910pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)Homo sapiensguanosine 5'-diphosphate7.5 mM MgCl2—0.041970pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)Homo sapiensguanosine 5'-triphosphate—0.064690pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)Homo sapiensoxaloacetatePEP-forming direction (OAA + GTP -> PEP + CO2 + GDP)—0.0331.21 × 10³pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)Homo sapiensoxaloacetateoxaloacetate decarboxylase activity (OAA -> pyruvate + CO2)—0.315pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)Homo sapiensoxaloacetateoxaloacetate decarboxylase activity (OAA -> pyruvate + CO2)—0.410pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)Homo sapiensphosphoenolpyruvateOAA-forming direction (PEP + GDP + HCO3- -> OAA + GTP)—0.217160pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)Homo sapiensphosphoenolpyruvatepyruvate kinase-like activity (PEP + GDP -> pyruvate + GTP)—1.92pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)variant Y235AHomo sapiensbicarbonate—6.83pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)variant Y235AHomo sapiensguanosine 5'-diphosphate2 mM MgCl2—0.034500pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)variant Y235AHomo sapiensguanosine 5'-diphosphate5 mM MgCl2—0.035490pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)variant Y235AHomo sapiensguanosine 5'-diphosphate7.5 mM MgCl2—0.034500pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)variant Y235AHomo sapiensguanosine 5'-triphosphate—0.05960pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)variant Y235AHomo sapiensoxaloacetatePEP-forming direction (OAA + GTP -> PEP + CO2 + GDP)—0.04770pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)variant Y235AHomo sapiensoxaloacetateoxaloacetate decarboxylase activity (OAA -> pyruvate + CO2)—0.31pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)variant Y235AHomo sapiensphosphoenolpyruvateOAA-forming direction (PEP + GDP + HCO3- -> OAA + GTP)—0.91930pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)variant Y235AHomo sapiensphosphoenolpyruvatepyruvate kinase-like activity (PEP + GDP -> pyruvate + GTP)—1.81pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)variant Y235FHomo sapiensbicarbonate—7.21pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)variant Y235FHomo sapiensguanosine 5'-diphosphate2 mM MgCl2—0.04770pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)variant Y235FHomo sapiensguanosine 5'-diphosphate5 mM MgCl2—0.0660pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)variant Y235FHomo sapiensguanosine 5'-diphosphate7.5 mM MgCl2—0.06750pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)variant Y235FHomo sapiensguanosine 5'-triphosphate—0.0261.8 × 10³pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)variant Y235FHomo sapiensoxaloacetatePEP-forming direction (OAA + GTP -> PEP + CO2 + GDP)—0.0381.17 × 10³pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)variant Y235FHomo sapiensoxaloacetateoxaloacetate decarboxylase activity (OAA -> pyruvate + CO2)—0.38pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)variant Y235FHomo sapiensoxaloacetateoxaloacetate decarboxylase activity (OAA -> pyruvate + CO2)—0.410pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)variant Y235FHomo sapiensphosphoenolpyruvateOAA-forming direction (PEP + GDP + HCO3- -> OAA + GTP)—0.03690pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)variant Y235FHomo sapiensphosphoenolpyruvatepyruvate kinase-like activity (PEP + GDP -> pyruvate + GTP)—0.32pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)variant Y235SHomo sapiensbicarbonate—7.52pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)variant Y235SHomo sapiensguanosine 5'-diphosphate2 mM MgCl2—0.044290pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)variant Y235SHomo sapiensguanosine 5'-diphosphate5 mM MgCl2—0.04290pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)variant Y235SHomo sapiensguanosine 5'-diphosphate7.5 mM MgCl2—0.053240pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)variant Y235SHomo sapiensguanosine 5'-triphosphate—0.04380pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)variant Y235SHomo sapiensoxaloacetatePEP-forming direction (OAA + GTP -> PEP + CO2 + GDP)—0.06160pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)variant Y235SHomo sapiensoxaloacetateoxaloacetate decarboxylase activity (OAA -> pyruvate + CO2)—0.30.4pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)variant Y235SHomo sapiensphosphoenolpyruvateOAA-forming direction (PEP + GDP + HCO3- -> OAA + GTP)—1.25610pH 7.2, 37 °COpen
cytosolic phosphoenolpyruvate carboxykinase (GTP-dependent PEPCK)variant Y235SHomo sapiensphosphoenolpyruvatepyruvate kinase-like activity (PEP + GDP -> pyruvate + GTP)—1.81pH 7.2, 37 °COpen