Values CatLog read from this paper
Engineering the substrate specificity and reactivity of a heme protein: creation of an ascorbate binding site in cytochrome c peroxidase
PMID 19061385doi:10.1021/bi801480rOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 24 records (48 values) from this paper: cytochrome c peroxidase (CcP) from Saccharomyces cerevisiae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 24 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| cytochrome c peroxidase (CcP) | Saccharomyces cerevisiae | 2-methoxyphenol | 4.1 | 53 | 0.077 calc. | pH 6, 25 °C | Open record: cytochrome c peroxidase (CcP), 2-methoxyphenol |
| cytochrome c peroxidase (CcP) | Saccharomyces cerevisiae | horse heart cytochrome c | 1.51 × 10³ | 0.093 | 1.6 × 10⁴ calc. | pH 6, 25 °C | Open record: cytochrome c peroxidase (CcP), horse heart cytochrome c |
| cytochrome c peroxidase (CcP) | Saccharomyces cerevisiae | L-ascorbic acid | 0.83 | 0.71 | 1.2 calc. | pH 6, 25 °C | Open record: cytochrome c peroxidase (CcP), L-ascorbic acid |
| cytochrome c peroxidase (CcP)variant N184R | Saccharomyces cerevisiae | 2-methoxyphenol | 3 | 27 | 0.11 calc. | pH 6, 25 °C | Open record: cytochrome c peroxidase (CcP) variant N184R, 2-methoxyphenol |
| cytochrome c peroxidase (CcP)variant N184R | Saccharomyces cerevisiae | horse heart cytochrome c | 570 | 0.14 | 4.1 × 10³ calc. | pH 6, 25 °C | Open record: cytochrome c peroxidase (CcP) variant N184R, horse heart cytochrome c |
| cytochrome c peroxidase (CcP)variant N184R | Saccharomyces cerevisiae | L-ascorbic acid | 1.5 | 1.7 | 0.88 calc. | pH 6, 25 °C | Open record: cytochrome c peroxidase (CcP) variant N184R, L-ascorbic acid |
| cytochrome c peroxidase (CcP)variant N184R/W191F | Saccharomyces cerevisiae | 2-methoxyphenol | 4.9 | 34 | 0.14 calc. | pH 6, 25 °C | Open record: cytochrome c peroxidase (CcP) variant N184R/W191F, 2-methoxyphenol |
| cytochrome c peroxidase (CcP)variant N184R/W191F | Saccharomyces cerevisiae | horse heart cytochrome c | 0.08 | 0.67 | 0.12 calc. | pH 6, 25 °C | Open record: cytochrome c peroxidase (CcP) variant N184R/W191F, horse heart cytochrome c |
| cytochrome c peroxidase (CcP)variant N184R/W191F | Saccharomyces cerevisiae | L-ascorbic acid | 0.27 | 0.31 | 0.87 calc. | pH 6, 25 °C | Open record: cytochrome c peroxidase (CcP) variant N184R/W191F, L-ascorbic acid |
| cytochrome c peroxidase (CcP)variant W191F | Saccharomyces cerevisiae | 2-methoxyphenol | 14 | 57 | 0.25 calc. | pH 6, 25 °C | Open record: cytochrome c peroxidase (CcP) variant W191F, 2-methoxyphenol |
| cytochrome c peroxidase (CcP)variant W191F | Saccharomyces cerevisiae | horse heart cytochrome c | 1.7 | 0.1 | 17 calc. | pH 6, 25 °C | Open record: cytochrome c peroxidase (CcP) variant W191F, horse heart cytochrome c |
| cytochrome c peroxidase (CcP)variant W191F | Saccharomyces cerevisiae | L-ascorbic acid | 0.25 | 0.45 | 0.56 calc. | pH 6, 25 °C | Open record: cytochrome c peroxidase (CcP) variant W191F, L-ascorbic acid |
| cytochrome c peroxidase (CcP)variant Y36A | Saccharomyces cerevisiae | 2-methoxyphenol | 5.4 | 36 | 0.15 calc. | pH 6, 25 °C | Open record: cytochrome c peroxidase (CcP) variant Y36A, 2-methoxyphenol |
| cytochrome c peroxidase (CcP)variant Y36A | Saccharomyces cerevisiae | horse heart cytochrome c | 580 | 0.23 | 2.5 × 10³ calc. | pH 6, 25 °C | Open record: cytochrome c peroxidase (CcP) variant Y36A, horse heart cytochrome c |
| cytochrome c peroxidase (CcP)variant Y36A | Saccharomyces cerevisiae | L-ascorbic acid | 1.3 | 1.7 | 0.76 calc. | pH 6, 25 °C | Open record: cytochrome c peroxidase (CcP) variant Y36A, L-ascorbic acid |
| cytochrome c peroxidase (CcP)variant Y36A/N184R | Saccharomyces cerevisiae | 2-methoxyphenol | 0.9 | 16 | 0.056 calc. | pH 6, 25 °C | Open record: cytochrome c peroxidase (CcP) variant Y36A/N184R, 2-methoxyphenol |
| cytochrome c peroxidase (CcP)variant Y36A/N184R | Saccharomyces cerevisiae | horse heart cytochrome c | 600 | 0.11 | 5.5 × 10³ calc. | pH 6, 25 °C | Open record: cytochrome c peroxidase (CcP) variant Y36A/N184R, horse heart cytochrome c |
| cytochrome c peroxidase (CcP)variant Y36A/N184R | Saccharomyces cerevisiae | L-ascorbic acid | 0.66 | 0.48 | 1.4 calc. | pH 6, 25 °C | Open record: cytochrome c peroxidase (CcP) variant Y36A/N184R, L-ascorbic acid |
| cytochrome c peroxidase (CcP)variant Y36A/N184R/W191F | Saccharomyces cerevisiae | 2-methoxyphenol | 1.5 | 14 | 0.11 calc. | pH 6, 25 °C | Open record: cytochrome c peroxidase (CcP) variant Y36A/N184R/W191F, 2-methoxyphenol |
| cytochrome c peroxidase (CcP)variant Y36A/N184R/W191F | Saccharomyces cerevisiae | horse heart cytochrome c | 1.6 | 0.3 | 5.3 calc. | pH 6, 25 °C | Open record: cytochrome c peroxidase (CcP) variant Y36A/N184R/W191F, horse heart cytochrome c |
| cytochrome c peroxidase (CcP)variant Y36A/N184R/W191F | Saccharomyces cerevisiae | L-ascorbic acid | 2.6 | 1.3 | 2.0 calc. | pH 6, 25 °C | Open record: cytochrome c peroxidase (CcP) variant Y36A/N184R/W191F, L-ascorbic acid |
| cytochrome c peroxidase (CcP)variant Y36A/W191F | Saccharomyces cerevisiae | 2-methoxyphenol | 3.2 | 45 | 0.071 calc. | pH 6, 25 °C | Open record: cytochrome c peroxidase (CcP) variant Y36A/W191F, 2-methoxyphenol |
| cytochrome c peroxidase (CcP)variant Y36A/W191F | Saccharomyces cerevisiae | horse heart cytochrome c | 0.06 | 0.16 | 0.38 calc. | pH 6, 25 °C | Open record: cytochrome c peroxidase (CcP) variant Y36A/W191F, horse heart cytochrome c |
| cytochrome c peroxidase (CcP)variant Y36A/W191F | Saccharomyces cerevisiae | L-ascorbic acid | 0.45 | 0.5 | 0.90 calc. | pH 6, 25 °C | Open record: cytochrome c peroxidase (CcP) variant Y36A/W191F, L-ascorbic acid |