CatLogenzyme kinetics catalog

Values CatLog read from this paper

Effects of inherited mutations on catalytic activity and structural stability of human glucose-6-phosphate isomerase expressed in Escherichia coli

PMID 19064002doi:10.1016/j.bbapap.2008.11.004Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 15 records (30 values) from this paper: glucose-6-phosphate isomerase (hGPI) from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 15 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 5.3.1.9

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
glucose-6-phosphate isomerase (hGPI)Homo sapiensD-fructose 6-phosphate6500.0371.8 × 10⁴ calc.pH 7.5, 30 °COpen
glucose-6-phosphate isomerase (hGPI)variant A300PHomo sapiensD-fructose 6-phosphate1000.0452.2 × 10³ calc.pH 7.5, 30 °COpen
glucose-6-phosphate isomerase (hGPI)variant E495KHomo sapiensD-fructose 6-phosphate4200.058.4 × 10³ calc.pH 7.5, 30 °COpen
glucose-6-phosphate isomerase (hGPI)variant I525THomo sapiensD-fructose 6-phosphate760.0342.2 × 10³ calc.pH 7.5, 30 °COpen
glucose-6-phosphate isomerase (hGPI)variant L339PHomo sapiensD-fructose 6-phosphate150.045330 calc.pH 7.5, 30 °COpen
glucose-6-phosphate isomerase (hGPI)variant L487FHomo sapiensD-fructose 6-phosphate190.039490 calc.pH 7.5, 30 °COpen
glucose-6-phosphate isomerase (hGPI)variant R347CHomo sapiensD-fructose 6-phosphate1500.0463.3 × 10³ calc.pH 7.5, 30 °COpen
glucose-6-phosphate isomerase (hGPI)variant R347HHomo sapiensD-fructose 6-phosphate420.0381.1 × 10³ calc.pH 7.5, 30 °COpen
glucose-6-phosphate isomerase (hGPI)variant R472HHomo sapiensD-fructose 6-phosphate1900.0682.8 × 10³ calc.pH 7.5, 30 °COpen
glucose-6-phosphate isomerase (hGPI)variant R75GHomo sapiensD-fructose 6-phosphate930.0382.4 × 10³ calc.pH 7.5, 30 °COpen
glucose-6-phosphate isomerase (hGPI)variant R83WHomo sapiensD-fructose 6-phosphate1900.0613.1 × 10³ calc.pH 7.5, 30 °COpen
glucose-6-phosphate isomerase (hGPI)variant S278LHomo sapiensD-fructose 6-phosphate0.60.03119 calc.pH 7.5, 30 °COpen
glucose-6-phosphate isomerase (hGPI)variant T195IHomo sapiensD-fructose 6-phosphate3000.0634.8 × 10³ calc.pH 7.5, 30 °COpen
glucose-6-phosphate isomerase (hGPI)variant T375RHomo sapiensD-fructose 6-phosphate750.0461.6 × 10³ calc.pH 7.5, 30 °COpen
glucose-6-phosphate isomerase (hGPI)variant V101MHomo sapiensD-fructose 6-phosphate1600.0632.5 × 10³ calc.pH 7.5, 30 °COpen