Values CatLog read from this paper
Mutations in the small subunit of ribulosebisphosphate carboxylase affect subunit binding and catalysis
PMID 1911767doi:10.1021/bi00105a029Open in Browse
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CatLog read 18 records (18 values) from this paper: ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme) from Synechococcus PCC 6301.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme) | Synechococcus PCC 6301 | carbon dioxidecarboxylase assay as in Andrews (1988) with 1 mg/mL BSA | 11.8 | — | — | pH 7.8 | Open record: ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme), carbon dioxide |
| ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme) | Synechococcus PCC 6301 | carbon dioxideenzyme preincubated 30 min with 40 mM MgCl2 and 30 mM NaHCO3 | — | 0.251 | — | pH 7.8 | Open record: ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme), carbon dioxide |
| ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme) | Synechococcus PCC 6301 | D-ribulose 1,5-bisphosphate | — | 0.0172 | — | pH 7.8 | Open record: ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme), D-ribulose 1,5-bisphosphate |
| ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme)variant T14A (small subunit) | Synechococcus PCC 6301 | carbon dioxidecarboxylase assay as in Andrews (1988) with 1 mg/mL BSA | 11.5 | — | — | pH 7.8 | Open record: ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme) variant T14A (small subunit), carbon dioxide |
| ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme)variant T14A (small subunit) | Synechococcus PCC 6301 | carbon dioxideenzyme preincubated 30 min with 40 mM MgCl2 and 30 mM NaHCO3 | — | 0.28 | — | pH 7.8 | Open record: ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme) variant T14A (small subunit), carbon dioxide |
| ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme)variant T14A (small subunit) | Synechococcus PCC 6301 | D-ribulose 1,5-bisphosphate | — | 0.0112 | — | pH 7.8 | Open record: ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme) variant T14A (small subunit), D-ribulose 1,5-bisphosphate |
| ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme)variant T14D (small subunit) | Synechococcus PCC 6301 | carbon dioxidecarboxylase assay as in Andrews (1988) with 1 mg/mL BSA | 4.9 | — | — | pH 7.8 | Open record: ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme) variant T14D (small subunit), carbon dioxide |
| ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme)variant T14D (small subunit) | Synechococcus PCC 6301 | carbon dioxideenzyme preincubated 30 min with 40 mM MgCl2 and 30 mM NaHCO3 | — | 0.24 | — | pH 7.8 | Open record: ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme) variant T14D (small subunit), carbon dioxide |
| ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme)variant T14D (small subunit) | Synechococcus PCC 6301 | D-ribulose 1,5-bisphosphate | — | 0.0109 | — | pH 7.8 | Open record: ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme) variant T14D (small subunit), D-ribulose 1,5-bisphosphate |
| ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme)variant T14G (small subunit) | Synechococcus PCC 6301 | carbon dioxidecarboxylase assay as in Andrews (1988) with 1 mg/mL BSA | 6 | — | — | pH 7.8 | Open record: ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme) variant T14G (small subunit), carbon dioxide |
| ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme)variant T14G (small subunit) | Synechococcus PCC 6301 | carbon dioxideenzyme preincubated 30 min with 40 mM MgCl2 and 30 mM NaHCO3 | — | 0.207 | — | pH 7.8 | Open record: ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme) variant T14G (small subunit), carbon dioxide |
| ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme)variant T14G (small subunit) | Synechococcus PCC 6301 | D-ribulose 1,5-bisphosphate | — | 0.0034 | — | pH 7.8 | Open record: ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme) variant T14G (small subunit), D-ribulose 1,5-bisphosphate |
| ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme)variant T14V (small subunit) | Synechococcus PCC 6301 | carbon dioxidecarboxylase assay as in Andrews (1988) with 1 mg/mL BSA | 11.3 | — | — | pH 7.8 | Open record: ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme) variant T14V (small subunit), carbon dioxide |
| ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme)variant T14V (small subunit) | Synechococcus PCC 6301 | carbon dioxideenzyme preincubated 30 min with 40 mM MgCl2 and 30 mM NaHCO3 | — | 0.226 | — | pH 7.8 | Open record: ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme) variant T14V (small subunit), carbon dioxide |
| ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme)variant T14V (small subunit) | Synechococcus PCC 6301 | D-ribulose 1,5-bisphosphate | — | 0.0125 | — | pH 7.8 | Open record: ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme) variant T14V (small subunit), D-ribulose 1,5-bisphosphate |
| ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme)variant Y17C (small subunit) | Synechococcus PCC 6301 | carbon dioxidecarboxylase assay as in Andrews (1988) with 1 mg/mL BSA | 6.7 | — | — | pH 7.8 | Open record: ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme) variant Y17C (small subunit), carbon dioxide |
| ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme)variant Y17C (small subunit) | Synechococcus PCC 6301 | carbon dioxideenzyme preincubated 30 min with 40 mM MgCl2 and 30 mM NaHCO3 | — | 0.273 | — | pH 7.8 | Open record: ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme) variant Y17C (small subunit), carbon dioxide |
| ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme)variant Y17C (small subunit) | Synechococcus PCC 6301 | D-ribulose 1,5-bisphosphate | — | 0.0112 | — | pH 7.8 | Open record: ribulose-1,5-bisphosphate carboxylase/oxygenase (L8S8 holoenzyme) variant Y17C (small subunit), D-ribulose 1,5-bisphosphate |