CatLogenzyme kinetics catalog

Values CatLog read from this paper

Closed complex of the D-3-hydroxybutyrate dehydrogenase induced by an enantiomeric competitive inhibitor

PMID 19122202doi:10.1093/jb/mvn186Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 52 records (130 values) from this paper: D-3-hydroxybutyrate dehydrogenase (HBDH) from Pseudomonas fragi.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.30

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
D-3-hydroxybutyrate dehydrogenase (HBDH)Pseudomonas fragi(R)-3-hydroxybutyrate3700.8463Open
D-3-hydroxybutyrate dehydrogenase (HBDH)Pseudomonas fragi(R)-3-hydroxybutyrateN-terminal His6-tag (pQE30 vector)3100.88352Open
D-3-hydroxybutyrate dehydrogenase (HBDH)Pseudomonas fragiacetoacetate1200.37324Open
D-3-hydroxybutyrate dehydrogenase (HBDH)Pseudomonas fragiNAD+with 25 mM D-3-HB—0.241.54 × 10³Open
D-3-hydroxybutyrate dehydrogenase (HBDH)Pseudomonas fragiNAD+N-terminal His6-tag (pQE30 vector)—0.171.82 × 10³Open
D-3-hydroxybutyrate dehydrogenase (HBDH)Pseudomonas fragiNADH—0.011.2 × 10⁴Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant H144APseudomonas fragi(R)-3-hydroxybutyrate21250.8Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant H144APseudomonas fragiNAD+—0.3560Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant K152RPseudomonas fragi(R)-3-hydroxybutyrate110313.5Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant K152RPseudomonas fragiNAD+—0.12917Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant L215APseudomonas fragi(R)-3-hydroxybutyrate709.27.6Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant L215APseudomonas fragiacetoacetate308.33.6Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant L215APseudomonas fragiNAD+—1.547Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant L215APseudomonas fragiNADH—0.560Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant L215VPseudomonas fragi(R)-3-hydroxybutyrate4353.4128Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant L215VPseudomonas fragiacetoacetate871.367Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant L215VPseudomonas fragiNAD+—0.291.5 × 10³Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant L215VPseudomonas fragiNADH—0.032.9 × 10³Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant Q196APseudomonas fragi(R)-3-hydroxybutyrate99472.1Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant Q196APseudomonas fragiNAD+—0.12825Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant Q196EPseudomonas fragi(R)-3-hydroxybutyrate83831Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant Q196EPseudomonas fragiNAD+—0.12692Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant Q196NPseudomonas fragi(R)-3-hydroxybutyrate22510.4Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant Q196NPseudomonas fragiNAD+—0.2110Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant Q94APseudomonas fragi(R)-3-hydroxybutyrate140304.7Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant Q94APseudomonas fragiNAD+—0.19737Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant T190APseudomonas fragi(R)-3-hydroxybutyrate22250.9Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant T190APseudomonas fragiacetoacetate4.5320.14Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant T190APseudomonas fragiNAD+—2.210Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant T190APseudomonas fragiNADH—0.145Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant T190CPseudomonas fragi(R)-3-hydroxybutyrate6.1360.2Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant T190CPseudomonas fragiacetoacetate5.17.70.66Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant T190CPseudomonas fragiNAD+—0.699Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant T190CPseudomonas fragiNADH—1.53.4Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant T190SPseudomonas fragi(R)-3-hydroxybutyrate7053.7191Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant T190SPseudomonas fragiacetoacetate1251.1114Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant T190SPseudomonas fragiNAD+—0.71993Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant T190SPseudomonas fragiNADH—0.026.25 × 10³Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant W187APseudomonas fragi(R)-3-hydroxybutyrate291000.3Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant W187APseudomonas fragiNAD+—0.7638Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant W187FPseudomonas fragi(R)-3-hydroxybutyrate3101.3238Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant W187FPseudomonas fragiNAD+—0.241.29 × 10³Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant W187TPseudomonas fragi(R)-3-hydroxybutyrate7.2840.09Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant W187TPseudomonas fragiNAD+—0.888.2Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant W187YPseudomonas fragi(R)-3-hydroxybutyrate1801.2150Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant W187YPseudomonas fragiNAD+—0.21857Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant W257APseudomonas fragi(R)-3-hydroxybutyrate6.7610.1Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant W257APseudomonas fragiNAD+—0.957Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant W257FPseudomonas fragi(R)-3-hydroxybutyrate88831.1Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant W257FPseudomonas fragiNAD+—0.49180Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant W257YPseudomonas fragi(R)-3-hydroxybutyrate12650.2Open
D-3-hydroxybutyrate dehydrogenase (HBDH)variant W257YPseudomonas fragiNAD+—0.7416Open