CatLogenzyme kinetics catalog

Values CatLog read from this paper

Improving thermostability and catalytic activity of pyranose 2-oxidase from Trametes multicolor by rational and semi-rational design

PMID 19143837doi:10.1111/j.1742-4658.2008.06823.xOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 54 records (162 values) from this paper: pyranose 2-oxidase (P2Ox) from Trametes multicolor.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.3.10

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
pyranose 2-oxidase (P2Ox)Trametes multicolor1,4-benzoquinoneD-glucose; cosubstrate D-glucose (100 mM)1520.241633pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)Trametes multicolor1,4-benzoquinoneD-galactose; cosubstrate D-galactose (100 mM)4.790.06574.2pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)Trametes multicolorD-galactose2.518.790.286pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)Trametes multicolorD-glucose48.10.93951.2pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)Trametes multicolorferricenium ionD-glucose; cosubstrate D-glucose (100 mM)1510.254592pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)Trametes multicolorferricenium ionD-galactose; cosubstrate D-galactose (100 mM)5.340.0777pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant E542KTrametes multicolor1,4-benzoquinoneD-glucose; cosubstrate D-glucose (100 mM)1890.1821039pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant E542KTrametes multicolor1,4-benzoquinoneD-galactose; cosubstrate D-galactose (100 mM)5.520.049113.3pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant E542KTrametes multicolorD-galactose2.593.870.67pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant E542KTrametes multicolorD-glucose35.90.52168.9pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant E542KTrametes multicolorferricenium ionD-glucose; cosubstrate D-glucose (100 mM)54.40.29187pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant E542KTrametes multicolorferricenium ionD-galactose; cosubstrate D-galactose (100 mM)1.440.06821.2pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant E542RTrametes multicolor1,4-benzoquinoneD-glucose; cosubstrate D-glucose (100 mM)1270.136932pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant E542RTrametes multicolor1,4-benzoquinoneD-galactose; cosubstrate D-galactose (100 mM)4.370.04109.1pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant E542RTrametes multicolorD-galactose1.994.260.467pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant E542RTrametes multicolorD-glucose28.50.48958.1pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant E542RTrametes multicolorferricenium ionD-glucose; cosubstrate D-glucose (100 mM)46.70.319147pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant E542RTrametes multicolorferricenium ionD-galactose; cosubstrate D-galactose (100 mM)2.080.18311.4pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537GTrametes multicolor1,4-benzoquinoneD-glucose; cosubstrate D-glucose (100 mM)1840.1761042pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537GTrametes multicolor1,4-benzoquinoneD-galactose; cosubstrate D-galactose (100 mM)4.640.04896.2pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537GTrametes multicolorD-galactose2.469.470.26pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537GTrametes multicolorD-glucose52.10.85161.2pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537GTrametes multicolorferricenium ionD-glucose; cosubstrate D-glucose (100 mM)2820.289975pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537GTrametes multicolorferricenium ionD-galactose; cosubstrate D-galactose (100 mM)7.070.08682.6pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537G/E542KTrametes multicolor1,4-benzoquinoneD-glucose; cosubstrate D-glucose (100 mM)1730.151157pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537G/E542KTrametes multicolor1,4-benzoquinoneD-galactose; cosubstrate D-galactose (100 mM)4.720.04118.4pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537G/E542KTrametes multicolorD-galactose2.346.010.389pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537G/E542KTrametes multicolorD-glucose43.90.48790.2pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537G/E542KTrametes multicolorferricenium ionD-glucose; cosubstrate D-glucose (100 mM)86.70.296294pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537G/E542KTrametes multicolorferricenium ionD-galactose; cosubstrate D-galactose (100 mM)2.110.07229.3pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537G/E542RTrametes multicolor1,4-benzoquinoneD-glucose; cosubstrate D-glucose (100 mM)1730.1551118pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537G/E542RTrametes multicolor1,4-benzoquinoneD-galactose; cosubstrate D-galactose (100 mM)4.750.037127pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537G/E542RTrametes multicolorD-galactose2.365.770.409pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537G/E542RTrametes multicolorD-glucose33.10.44175pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537G/E542RTrametes multicolorferricenium ionD-glucose; cosubstrate D-glucose (100 mM)1020.328309pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537G/E542RTrametes multicolorferricenium ionD-galactose; cosubstrate D-galactose (100 mM)1.810.05433.8pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537WTrametes multicolor1,4-benzoquinoneD-glucose; cosubstrate D-glucose (100 mM)2050.131579pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537WTrametes multicolor1,4-benzoquinoneD-galactose; cosubstrate D-galactose (100 mM)5.370.036150.1pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537WTrametes multicolorD-galactose2.99.40.309pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537WTrametes multicolorD-glucose590.74978.8pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537WTrametes multicolorferricenium ionD-glucose; cosubstrate D-glucose (100 mM)3340.2531.32 × 10³pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537WTrametes multicolorferricenium ionD-galactose; cosubstrate D-galactose (100 mM)8.180.063130.2pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537W/E542KTrametes multicolor1,4-benzoquinoneD-glucose; cosubstrate D-glucose (100 mM)1810.141292pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537W/E542KTrametes multicolor1,4-benzoquinoneD-galactose; cosubstrate D-galactose (100 mM)5.090.038135.4pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537W/E542KTrametes multicolorD-galactose2.515.190.483pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537W/E542KTrametes multicolorD-glucose46.50.432107.6pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537W/E542KTrametes multicolorferricenium ionD-glucose; cosubstrate D-glucose (100 mM)1270.408310pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537W/E542KTrametes multicolorferricenium ionD-galactose; cosubstrate D-galactose (100 mM)2.680.0929.8pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537W/E542RTrametes multicolor1,4-benzoquinoneD-glucose; cosubstrate D-glucose (100 mM)1750.1371278pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537W/E542RTrametes multicolor1,4-benzoquinoneD-galactose; cosubstrate D-galactose (100 mM)4.770.032148pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537W/E542RTrametes multicolorD-galactose2.485.490.452pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537W/E542RTrametes multicolorD-glucose31.70.41975.6pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537W/E542RTrametes multicolorferricenium ionD-glucose; cosubstrate D-glucose (100 mM)86.30.281307pH 6.5, 30 °COpen
pyranose 2-oxidase (P2Ox)variant L537W/E542RTrametes multicolorferricenium ionD-galactose; cosubstrate D-galactose (100 mM)2.470.07433.4pH 6.5, 30 °COpen