Values CatLog read from this paper
High tolerance to simultaneous active-site mutations in TEM-1 beta-lactamase: Distinct mutational paths provide more generalized beta-lactam recognition
PMID 19177359doi:10.1002/pro.25Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 27 records (54 values) from this paper: TEM-1 β-lactamase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 27 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| TEM-1 β-lactamase | Escherichia coli | benzylpenicillin | 1.66 × 10³ | 0.062 | 2.7 × 10⁴ calc. | pH 7 | Open record: TEM-1 β-lactamase, benzylpenicillin |
| TEM-1 β-lactamase | Escherichia coli | cefazolin | 69 | 0.13 | 530 calc. | pH 7 | Open record: TEM-1 β-lactamase, cefazolin |
| TEM-1 β-lactamase | Escherichia coli | cefotaxime | 0.74 | 0.84 | 0.88 calc. | pH 7 | Open record: TEM-1 β-lactamase, cefotaxime |
| TEM-1 β-lactamase | Escherichia coli | cephalothin | 77 | 0.18 | 430 calc. | pH 7 | Open record: TEM-1 β-lactamase, cephalothin |
| TEM-1 β-lactamasevariant E104P/Y105H/G238S | Escherichia coli | benzylpenicillin | 20 | 0.031 | 650 calc. | pH 7 | Open record: TEM-1 β-lactamase variant E104P/Y105H/G238S, benzylpenicillin |
| TEM-1 β-lactamasevariant E104P/Y105H/G238S | Escherichia coli | cefazolin | 88 | 0.061 | 1.4 × 10³ calc. | pH 7 | Open record: TEM-1 β-lactamase variant E104P/Y105H/G238S, cefazolin |
| TEM-1 β-lactamasevariant E104P/Y105H/G238S | Escherichia coli | cefotaxime | 61 | 0.11 | 550 calc. | pH 7 | Open record: TEM-1 β-lactamase variant E104P/Y105H/G238S, cefotaxime |
| TEM-1 β-lactamasevariant E104P/Y105H/G238S | Escherichia coli | cephalothin | 31 | 0.007 | 4.4 × 10³ calc. | pH 7 | Open record: TEM-1 β-lactamase variant E104P/Y105H/G238S, cephalothin |
| TEM-1 β-lactamasevariant E104S/Y105W/G238S/E240S | Escherichia coli | cefazolin | 50 | 0.055 | 910 calc. | pH 7 | Open record: TEM-1 β-lactamase variant E104S/Y105W/G238S/E240S, cefazolin |
| TEM-1 β-lactamasevariant E104S/Y105W/G238S/E240S | Escherichia coli | cefotaxime | 20 | 0.04 | 500 calc. | pH 7 | Open record: TEM-1 β-lactamase variant E104S/Y105W/G238S/E240S, cefotaxime |
| TEM-1 β-lactamasevariant E104S/Y105W/G238S/E240S | Escherichia coli | cephalothin | 24 | 0.01 | 2.4 × 10³ calc. | pH 7 | Open record: TEM-1 β-lactamase variant E104S/Y105W/G238S/E240S, cephalothin |
| TEM-1 β-lactamasevariant E104V/Y105N/G238S/E240L | Escherichia coli | benzylpenicillin | 29 | 0.015 | 1.9 × 10³ calc. | pH 7 | Open record: TEM-1 β-lactamase variant E104V/Y105N/G238S/E240L, benzylpenicillin |
| TEM-1 β-lactamasevariant E104V/Y105N/G238S/E240L | Escherichia coli | cefazolin | 41 | 0.028 | 1.5 × 10³ calc. | pH 7 | Open record: TEM-1 β-lactamase variant E104V/Y105N/G238S/E240L, cefazolin |
| TEM-1 β-lactamasevariant E104V/Y105N/G238S/E240L | Escherichia coli | cefotaxime | 20 | 0.028 | 710 calc. | pH 7 | Open record: TEM-1 β-lactamase variant E104V/Y105N/G238S/E240L, cefotaxime |
| TEM-1 β-lactamasevariant E104V/Y105N/G238S/E240L | Escherichia coli | cephalothin | 16 | 0.005 | 3.2 × 10³ calc. | pH 7 | Open record: TEM-1 β-lactamase variant E104V/Y105N/G238S/E240L, cephalothin |
| TEM-1 β-lactamasevariant E104V/Y105N/G238S/E240T | Escherichia coli | benzylpenicillin | 19 | 0.017 | 1.1 × 10³ calc. | pH 7 | Open record: TEM-1 β-lactamase variant E104V/Y105N/G238S/E240T, benzylpenicillin |
| TEM-1 β-lactamasevariant E104V/Y105N/G238S/E240T | Escherichia coli | cefazolin | 38 | 0.083 | 460 calc. | pH 7 | Open record: TEM-1 β-lactamase variant E104V/Y105N/G238S/E240T, cefazolin |
| TEM-1 β-lactamasevariant E104V/Y105N/G238S/E240T | Escherichia coli | cefotaxime | 23 | 0.072 | 320 calc. | pH 7 | Open record: TEM-1 β-lactamase variant E104V/Y105N/G238S/E240T, cefotaxime |
| TEM-1 β-lactamasevariant E104V/Y105N/G238S/E240T | Escherichia coli | cephalothin | 10 | 0.016 | 620 calc. | pH 7 | Open record: TEM-1 β-lactamase variant E104V/Y105N/G238S/E240T, cephalothin |
| TEM-1 β-lactamasevariant E240H | Escherichia coli | benzylpenicillin | 2 × 10³ | 0.42 | 4.8 × 10³ calc. | pH 7 | Open record: TEM-1 β-lactamase variant E240H, benzylpenicillin |
| TEM-1 β-lactamasevariant E240H | Escherichia coli | cefazolin | 118 | 0.095 | 1.2 × 10³ calc. | pH 7 | Open record: TEM-1 β-lactamase variant E240H, cefazolin |
| TEM-1 β-lactamasevariant E240H | Escherichia coli | cefotaxime | 2.7 | 0.62 | 4.4 calc. | pH 7 | Open record: TEM-1 β-lactamase variant E240H, cefotaxime |
| TEM-1 β-lactamasevariant E240H | Escherichia coli | cephalothin | 63 | 0.023 | 2.7 × 10³ calc. | pH 7 | Open record: TEM-1 β-lactamase variant E240H, cephalothin |
| TEM-1 β-lactamasevariant G238N/E240H | Escherichia coli | benzylpenicillin | 82 | 0.106 | 770 calc. | pH 7 | Open record: TEM-1 β-lactamase variant G238N/E240H, benzylpenicillin |
| TEM-1 β-lactamasevariant G238N/E240H | Escherichia coli | cefazolin | 82 | 0.12 | 680 calc. | pH 7 | Open record: TEM-1 β-lactamase variant G238N/E240H, cefazolin |
| TEM-1 β-lactamasevariant G238N/E240H | Escherichia coli | cefotaxime | 55 | 0.061 | 900 calc. | pH 7 | Open record: TEM-1 β-lactamase variant G238N/E240H, cefotaxime |
| TEM-1 β-lactamasevariant G238N/E240H | Escherichia coli | cephalothin | 31 | 0.011 | 2.8 × 10³ calc. | pH 7 | Open record: TEM-1 β-lactamase variant G238N/E240H, cephalothin |