Values CatLog read from this paper
Enzymatic activity and thermal stability of PEG-alpha-chymotrypsin conjugates
PMID 19224136doi:10.1007/s10529-009-9947-yOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 13 records (26 values) from this paper: α-chymotrypsin from Bos taurus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 13 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| α-chymotrypsin | Bos taurus | N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide | 12.1 | 0.05 | 240 calc. | pH 7.1, 25 °C | Open record: α-chymotrypsin, N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide |
| α-chymotrypsinvariant PEG2000-conjugated, 1.3 ± 0.6 PEG per α-CT | Bos taurus | N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide | 13.5 | 0.08 | 170 calc. | pH 7.1, 25 °C | Open record: α-chymotrypsin variant PEG2000-conjugated, 1.3 ± 0.6 PEG per α-CT, N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide |
| α-chymotrypsinvariant PEG2000-conjugated, 2.2 ± 0.2 PEG per α-CT | Bos taurus | N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide | 10.7 | 0.07 | 150 calc. | pH 7.1, 25 °C | Open record: α-chymotrypsin variant PEG2000-conjugated, 2.2 ± 0.2 PEG per α-CT, N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide |
| α-chymotrypsinvariant PEG2000-conjugated, 4.2 ± 0.3 PEG per α-CT | Bos taurus | N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide | 7.6 | 0.09 | 84 calc. | pH 7.1, 25 °C | Open record: α-chymotrypsin variant PEG2000-conjugated, 4.2 ± 0.3 PEG per α-CT, N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide |
| α-chymotrypsinvariant PEG2000-conjugated, 8.4 ± 0.4 PEG per α-CT | Bos taurus | N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide | 9.4 | 0.15 | 63 calc. | pH 7.1, 25 °C | Open record: α-chymotrypsin variant PEG2000-conjugated, 8.4 ± 0.4 PEG per α-CT, N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide |
| α-chymotrypsinvariant PEG2000-conjugated, 9.1 ± 0.3 PEG per α-CT | Bos taurus | N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide | 7.9 | 0.19 | 42 calc. | pH 7.1, 25 °C | Open record: α-chymotrypsin variant PEG2000-conjugated, 9.1 ± 0.3 PEG per α-CT, N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide |
| α-chymotrypsinvariant PEG5000-conjugated, 1.3 ± 0.3 PEG per α-CT | Bos taurus | N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide | 13.1 | 0.1 | 130 calc. | pH 7.1, 25 °C | Open record: α-chymotrypsin variant PEG5000-conjugated, 1.3 ± 0.3 PEG per α-CT, N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide |
| α-chymotrypsinvariant PEG5000-conjugated, 2.9 ± 0.3 PEG per α-CT | Bos taurus | N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide | 10.2 | 0.08 | 130 calc. | pH 7.1, 25 °C | Open record: α-chymotrypsin variant PEG5000-conjugated, 2.9 ± 0.3 PEG per α-CT, N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide |
| α-chymotrypsinvariant PEG5000-conjugated, 4.2 ± 0.4 PEG per α-CT | Bos taurus | N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide | 8.7 | 0.08 | 110 calc. | pH 7.1, 25 °C | Open record: α-chymotrypsin variant PEG5000-conjugated, 4.2 ± 0.4 PEG per α-CT, N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide |
| α-chymotrypsinvariant PEG5000-conjugated, 6.0 ± 0.6 PEG per α-CT | Bos taurus | N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide | 8.3 | 0.08 | 100 calc. | pH 7.1, 25 °C | Open record: α-chymotrypsin variant PEG5000-conjugated, 6.0 ± 0.6 PEG per α-CT, N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide |
| α-chymotrypsinvariant PEG5000-conjugated, 7.6 ± 0.7 PEG per α-CT | Bos taurus | N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide | 8.6 | 0.11 | 78 calc. | pH 7.1, 25 °C | Open record: α-chymotrypsin variant PEG5000-conjugated, 7.6 ± 0.7 PEG per α-CT, N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide |
| α-chymotrypsinvariant PEG700-conjugated, 3.8 ± 0.2 PEG per α-CT | Bos taurus | N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide | 11.6 | 0.07 | 170 calc. | pH 7.1, 25 °C | Open record: α-chymotrypsin variant PEG700-conjugated, 3.8 ± 0.2 PEG per α-CT, N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide |
| α-chymotrypsinvariant PEG700-conjugated, 7.9 ± 0.4 PEG per α-CT | Bos taurus | N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide | 6.2 | 0.15 | 41 calc. | pH 7.1, 25 °C | Open record: α-chymotrypsin variant PEG700-conjugated, 7.9 ± 0.4 PEG per α-CT, N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-4-nitroanilide |