CatLogenzyme kinetics catalog

Values CatLog read from this paper

Residue Phe112 of the human-type corrinoid adenosyltransferase (PduO) enzyme of Lactobacillus reuteri is critical to the formation of the four-coordinate Co(II) corrinoid substrate and to the…

PMID 19236001doi:10.1021/bi9000134Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 28 records (84 values) from this paper: ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase) from Lactobacillus reuteri.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.5.1.17

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase)Lactobacillus reuteriadenosine 5'-triphosphate0.0290.00555.5pH 8, 37 °COpen
ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase)Lactobacillus reutericob(II)alamin0.0380.00784.8pH 8, 37 °COpen
ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase)variant F112ALactobacillus reuteriadenosine 5'-triphosphate0.00560.03460.17pH 8, 37 °COpen
ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase)variant F112ALactobacillus reutericob(I)alamin0.00660.00193.6pH 8, 37 °COpen
ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase)variant F112HLactobacillus reuteriadenosine 5'-triphosphateKCl 0.1 M; AdoCbl formation at 525 nm5.9 × 10⁻⁴0.01040.057pH 8, 37 °COpen
ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase)variant F112HLactobacillus reuteriadenosine 5'-triphosphatecontinuous spectrophotometric assay3.8 × 10⁻⁴0.00260.14pH 8, 37 °COpen
ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase)variant F112HLactobacillus reutericob(I)alamin4.2 × 10⁻⁴0.00280.15pH 8, 37 °COpen
ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase)variant F112HLactobacillus reutericob(II)alamin6.7 × 10⁻⁴0.0530.013pH 8, 37 °COpen
ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase)variant F112WLactobacillus reuteriadenosine 5'-triphosphateKCl 0.1 M; AdoCbl formation at 525 nm0.00280.00730.39pH 8, 37 °COpen
ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase)variant F112WLactobacillus reuteriadenosine 5'-triphosphatecontinuous spectrophotometric assay0.00180.0020.98pH 8, 37 °COpen
ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase)variant F112WLactobacillus reutericob(I)alamin0.0027.7 × 10⁻⁴2.6pH 8, 37 °COpen
ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase)variant F112WLactobacillus reutericob(II)alamin0.00320.02880.11pH 8, 37 °COpen
ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase)variant F112YLactobacillus reuteriadenosine 5'-triphosphateKCl 0.1 M; AdoCbl formation at 525 nm0.00230.00940.25pH 8, 37 °COpen
ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase)variant F112YLactobacillus reuteriadenosine 5'-triphosphatecontinuous spectrophotometric assay0.00160.00121.4pH 8, 37 °COpen
ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase)variant F112YLactobacillus reutericob(I)alamin0.00175.5 × 10⁻⁴3.1pH 8, 37 °COpen
ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase)variant F112YLactobacillus reutericob(II)alamin0.00270.03420.083pH 8, 37 °COpen
ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase)variant F163ALactobacillus reuteriadenosine 5'-triphosphateKCl 0.1 M; AdoCbl formation at 525 nm0.0150.09610.16pH 8, 37 °COpen
ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase)variant F163ALactobacillus reuteriadenosine 5'-triphosphatecontinuous spectrophotometric assay0.0150.01591.8pH 8, 37 °COpen
ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase)variant F163ALactobacillus reutericob(I)alamin0.0151.9 × 10⁻⁴79pH 8, 37 °COpen
ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase)variant F163ALactobacillus reutericob(II)alamin0.0270.1340.2pH 8, 37 °COpen
ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase)variant F187ALactobacillus reuteriadenosine 5'-triphosphateKCl 0.1 M; AdoCbl formation at 525 nm0.0140.00791.8pH 8, 37 °COpen
ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase)variant F187ALactobacillus reuteriadenosine 5'-triphosphatecontinuous spectrophotometric assay0.0210.001217pH 8, 37 °COpen
ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase)variant F187ALactobacillus reutericob(I)alamin0.0181.1 × 10⁻⁴160pH 8, 37 °COpen
ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase)variant F187ALactobacillus reutericob(II)alamin0.0150.01580.93pH 8, 37 °COpen
ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase)variant Δ183-188Lactobacillus reuteriadenosine 5'-triphosphateKCl 0.1 M; AdoCbl formation at 525 nm0.0170.00991.7pH 8, 37 °COpen
ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase)variant Δ183-188Lactobacillus reuteriadenosine 5'-triphosphatecontinuous spectrophotometric assay0.0230.00317.5pH 8, 37 °COpen
ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase)variant Δ183-188Lactobacillus reutericob(I)alamin0.027 × 10⁻⁵395pH 8, 37 °COpen
ATP:Co(I)rrinoid adenosyltransferase PduO (LrPduO; human-type corrinoid adenosyltransferase)variant Δ183-188Lactobacillus reutericob(II)alamin0.0180.01631.1pH 8, 37 °COpen