Values CatLog read from this paper
Cloning and characterization of Rhodotorula glutinis thymine hydroxylase
PMID 19341313doi:10.1021/tx8004482Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 8 records (21 values) from this paper: thymine hydroxylase from Rhodotorula glutinis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 3 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| thymine hydroxylase | Rhodotorula glutinis | 5-formyluracil | 1.13 | 1.7 | 0.67 | pH 7.5, 30 °C | Open record: thymine hydroxylase, 5-formyluracil |
| thymine hydroxylase | Rhodotorula glutinis | 5-hydroxymethyluracil | 0.42 | 0.23 | 1.8 | pH 7.5, 30 °C | Open record: thymine hydroxylase, 5-hydroxymethyluracil |
| thymine hydroxylase | Rhodotorula glutinis | N1-methylthymine | 0.43 | 1.3 | 0.33 | pH 7.5, 30 °C | Open record: thymine hydroxylase, N1-methylthymine |
| thymine hydroxylase | Rhodotorula glutinis | N1-methyluracil | 0.27 | 0.11 | 2.5 | pH 7.5, 30 °C | Open record: thymine hydroxylase, N1-methyluracil |
| thymine hydroxylase | Rhodotorula glutinis | thymine | 1.12 | 0.145 | 9.3 | pH 7.5, 30 °C | Open record: thymine hydroxylase, thymine |
| thymine hydroxylasevariant N187D | Rhodotorula glutinis | thymine | 0.248 | 0.067 | 3.7 calc. | pH 7.5, 30 °C | Open record: thymine hydroxylase variant N187D, thymine |
| thymine hydroxylasevariant N250D | Rhodotorula glutinis | thymine | 0.0112 | 1.15 | 0.00970 calc. | pH 7.5, 30 °C | Open record: thymine hydroxylase variant N250D, thymine |
| thymine hydroxylasevariant N293D | Rhodotorula glutinis | thymine | 0.053 | 2.1 | 0.025 calc. | pH 7.5, 30 °C | Open record: thymine hydroxylase variant N293D, thymine |