CatLogenzyme kinetics catalog

Values CatLog read from this paper

The functional role of a conserved loop in EAL domain-based cyclic di-GMP-specific phosphodiesterase

PMID 19376848doi:10.1128/jb.00327-09Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 7 records (19 values) from this paper: RocR, PA2567, DGC2 (diguanylate cyclase 2) EAL domain from Pseudomonas aeruginosa, Acetobacter xylinus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.

EC 3.1.4.52

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
DGC2 (diguanylate cyclase 2) EAL domainvariant N473D/K476T/I478YAcetobacter xylinuscyclic di-GMP5.9 × 10⁻⁴0.0130.045 calc.Open
PA2567Pseudomonas aeruginosacyclic di-GMP—0.005275pH 8, 23 °COpen
PA2567variant E464APseudomonas aeruginosacyclic di-GMP1.10.01384pH 8, 23 °COpen
RocRvariant D56NPseudomonas aeruginosacyclic di-GMP0.132 × 10⁻⁴650pH 8, 23 °COpen
RocRvariant D56N/D296APseudomonas aeruginosacyclic di-GMP0.0120.00942.1pH 8, 23 °COpen
RocRvariant F297APseudomonas aeruginosacyclic di-GMP0.027 × 10⁻⁴28pH 8, 23 °COpen
RocRvariant S302APseudomonas aeruginosacyclic di-GMP0.140.005525pH 8, 23 °COpen