CatLogenzyme kinetics catalog

Values CatLog read from this paper

Replication past the N5-methyl-formamidopyrimidine lesion of deoxyguanosine by DNA polymerases and an improved procedure for sequence analysis of in vitro bypass products by mass spectrometry

PMID 19397282doi:10.1021/tx900047cOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 24 records (72 values) from this paper: DNA polymerase I Klenow fragment (Kf-), DNA polymerase IV (Dpo4) from Escherichia coli, Sulfolobus solfataricus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.7.7.7

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
DNA polymerase I Klenow fragment (Kf-)variant exonuclease-deficient (exo-)Escherichia coli2'-deoxyadenosine 5'-triphosphateTable 10.00160.00121.3Open
DNA polymerase I Klenow fragment (Kf-)variant exonuclease-deficient (exo-)Escherichia coli2'-deoxyadenosine 5'-triphosphateunmodified dG 28mer template (local 5'-T-dG-G-3'); Table 10.00550.00282Open
DNA polymerase I Klenow fragment (Kf-)variant exonuclease-deficient (exo-)Escherichia coli2'-deoxyadenosine 5'-triphosphateTable 20.00180.00590.3Open
DNA polymerase I Klenow fragment (Kf-)variant exonuclease-deficient (exo-)Escherichia coli2'-deoxyadenosine 5'-triphosphateunmodified dG 29mer template (local 5'-T-dG-T-3'); Table 20.0010.00380.3Open
DNA polymerase I Klenow fragment (Kf-)variant exonuclease-deficient (exo-)Escherichia coli2'-deoxycytidine 5'-triphosphateTable 10.0026.6 × 10⁻⁵30Open
DNA polymerase I Klenow fragment (Kf-)variant exonuclease-deficient (exo-)Escherichia coli2'-deoxycytidine 5'-triphosphateunmodified dG 28mer template (local 5'-T-dG-G-3'); Table 10.00331.3 × 10⁻⁵250Open
DNA polymerase I Klenow fragment (Kf-)variant exonuclease-deficient (exo-)Escherichia coli2'-deoxycytidine 5'-triphosphateTable 26.6 × 10⁻⁴1.6 × 10⁻⁴4.1Open
DNA polymerase I Klenow fragment (Kf-)variant exonuclease-deficient (exo-)Escherichia coli2'-deoxycytidine 5'-triphosphateunmodified dG 29mer template (local 5'-T-dG-T-3'); Table 20.00212.6 × 10⁻⁵66Open
DNA polymerase I Klenow fragment (Kf-)variant exonuclease-deficient (exo-)Escherichia coli2'-deoxyguanosine 5'-triphosphate0.00171.9 × 10⁻⁴8.9Open
DNA polymerase I Klenow fragment (Kf-)variant exonuclease-deficient (exo-)Escherichia coli2'-deoxyguanosine 5'-triphosphateunmodified dG 28mer template (local 5'-T-dG-G-3')0.00520.00212.5Open
DNA polymerase I Klenow fragment (Kf-)variant exonuclease-deficient (exo-)Escherichia colithymidine 5'-triphosphate0.00283.3 × 10⁻⁴8.5Open
DNA polymerase I Klenow fragment (Kf-)variant exonuclease-deficient (exo-)Escherichia colithymidine 5'-triphosphateunmodified dG 28mer template (local 5'-T-dG-G-3')0.00120.00340.4Open
DNA polymerase IV (Dpo4)Sulfolobus solfataricus2'-deoxyadenosine 5'-triphosphateTable 12 × 10⁻⁴0.0070.03Open
DNA polymerase IV (Dpo4)Sulfolobus solfataricus2'-deoxyadenosine 5'-triphosphateTable 12.5 × 10⁻⁴0.0220.01Open
DNA polymerase IV (Dpo4)Sulfolobus solfataricus2'-deoxyadenosine 5'-triphosphateTable 22.4 × 10⁻⁴0.00880.03Open
DNA polymerase IV (Dpo4)Sulfolobus solfataricus2'-deoxyadenosine 5'-triphosphateTable 22.9 × 10⁻⁴0.030.01Open
DNA polymerase IV (Dpo4)Sulfolobus solfataricus2'-deoxycytidine 5'-triphosphateTable 12.7 × 10⁻⁴9.8 × 10⁻⁵2.8Open
DNA polymerase IV (Dpo4)Sulfolobus solfataricus2'-deoxycytidine 5'-triphosphateTable 13 × 10⁻⁴7.7 × 10⁻⁵3.9Open
DNA polymerase IV (Dpo4)Sulfolobus solfataricus2'-deoxycytidine 5'-triphosphateTable 25.3 × 10⁻⁴7.1 × 10⁻⁴0.7Open
DNA polymerase IV (Dpo4)Sulfolobus solfataricus2'-deoxycytidine 5'-triphosphateTable 22.4 × 10⁻⁴2 × 10⁻⁴1.2Open
DNA polymerase IV (Dpo4)Sulfolobus solfataricus2'-deoxyguanosine 5'-triphosphate6 × 10⁻⁴0.0130.05Open
DNA polymerase IV (Dpo4)Sulfolobus solfataricus2'-deoxyguanosine 5'-triphosphateunmodified dG 28mer template (local 5'-T-dG-G-3')4.3 × 10⁻⁴0.0490.01Open
DNA polymerase IV (Dpo4)Sulfolobus solfataricusthymidine 5'-triphosphate1.3 × 10⁻⁴0.0060.02Open
DNA polymerase IV (Dpo4)Sulfolobus solfataricusthymidine 5'-triphosphateunmodified dG 28mer template (local 5'-T-dG-G-3')2.9 × 10⁻⁴0.0290.01Open