CatLogenzyme kinetics catalog

Values CatLog read from this paper

Reversing evolution: re-establishing obligate metal ion dependence in a metal-independent KDO8P synthase

PMID 19447118doi:10.1016/j.jmb.2009.05.014Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 36 records (90 values) from this paper: 3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS) from Neisseria meningitidis.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.5.1.55

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)Neisseria meningitidisarabinose 5-phosphate—0.00571.4 × 10³pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)Neisseria meningitidisphosphoenol pyruvate80.00253.2 × 10³pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant C246SNeisseria meningitidisarabinose 5-phosphate—0.21326pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant C246SNeisseria meningitidisphosphoenol pyruvate5.50.0093591pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant D247ENeisseria meningitidisarabinose 5-phosphate—0.0673pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant D247ENeisseria meningitidisphosphoenol pyruvate4.40.0049898pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant D247E/P249ANeisseria meningitidisarabinose 5-phosphate—0.07449pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant D247E/P249ANeisseria meningitidisphosphoenol pyruvate3.60.0085424pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant N23CNeisseria meningitidisarabinose 5-phosphate20 µM EDTA; no added metal ion—0.0616pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant N23CNeisseria meningitidisarabinose 5-phosphate90 µM MnSO4—0.012118pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant N23CNeisseria meningitidisphosphoenol pyruvate20 µM EDTA; no added metal ion0.930.0028332pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant N23CNeisseria meningitidisphosphoenol pyruvate90 µM MnSO41.420.0054263pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant N23C/C246SNeisseria meningitidisarabinose 5-phosphate20 µM EDTA; no added metal ion—1.040.02pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant N23C/C246SNeisseria meningitidisarabinose 5-phosphate90 µM MnSO4—0.09728pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant N23C/C246SNeisseria meningitidisphosphoenol pyruvate20 µM EDTA; no added metal ion0.020.050.4pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant N23C/C246SNeisseria meningitidisphosphoenol pyruvate90 µM MnSO42.710.0066411pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant N23C/C246S/D247ENeisseria meningitidisarabinose 5-phosphate—0.4987.9pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant N23C/C246S/D247ENeisseria meningitidisphosphoenol pyruvate3.90.0153257pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant N23C/C246S/D247E/P249ANeisseria meningitidisarabinose 5-phosphate—0.548.1pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant N23C/C246S/D247E/P249ANeisseria meningitidisphosphoenol pyruvate4.40.022200pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant N23C/C246S/P249ANeisseria meningitidisarabinose 5-phosphate—0.1925pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant N23C/C246S/P249ANeisseria meningitidisphosphoenol pyruvate4.790.0095504pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant N23C/D247ENeisseria meningitidisarabinose 5-phosphate20 µM EDTA; no added metal ion—0.241.6pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant N23C/D247ENeisseria meningitidisarabinose 5-phosphate90 µM MnSO4—0.1423pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant N23C/D247ENeisseria meningitidisphosphoenol pyruvate20 µM EDTA; no added metal ion0.380.007749pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant N23C/D247ENeisseria meningitidisphosphoenol pyruvate90 µM MnSO43.270.00241363pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant N23C/D247E/P249ANeisseria meningitidisarabinose 5-phosphate20 µM EDTA; no added metal ion—0.330.9pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant N23C/D247E/P249ANeisseria meningitidisarabinose 5-phosphate90 µM MnSO4—0.10719pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant N23C/D247E/P249ANeisseria meningitidisphosphoenol pyruvate20 µM EDTA; no added metal ion0.310.01718pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant N23C/D247E/P249ANeisseria meningitidisphosphoenol pyruvate90 µM MnSO42.020.0027748pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant N23C/P249ANeisseria meningitidisarabinose 5-phosphate20 µM EDTA; no added metal ion—0.1093.5pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant N23C/P249ANeisseria meningitidisarabinose 5-phosphate90 µM MnSO4—0.01372pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant N23C/P249ANeisseria meningitidisphosphoenol pyruvate20 µM EDTA; no added metal ion0.380.0033115pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant N23C/P249ANeisseria meningitidisphosphoenol pyruvate90 µM MnSO40.940.0038247pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant P249ANeisseria meningitidisarabinose 5-phosphate—0.012717pH 7.4, 37 °COpen
3-deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS)variant P249ANeisseria meningitidisphosphoenol pyruvate8.60.00471.83 × 10³pH 7.4, 37 °COpen