Values CatLog read from this paper
Determination of key residues for catalysis and RNA cleavage specificity: one mutation turns RNase II into a "SUPER-ENZYME"
PMID 19458082doi:10.1074/jbc.m109.020693Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 2 records (5 values) from this paper: exoribonuclease II (RNase II) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| exoribonuclease II (RNase II) | Escherichia coli | poly(A) oligoribonucleotide, 35 nt (5'-32P-labelled) | 0.41 | 0.00125 | 230 | pH 8, 37 °C | Open record: exoribonuclease II (RNase II), poly(A) oligoribonucleotide, 35 nt (5'-32P-labelled) |
| exoribonuclease II (RNase II)variant E542A | Escherichia coli | poly(A) oligoribonucleotide, 35 nt (5'-32P-labelled) | 8.08 × 10⁴ | 3 × 10⁻⁴ | 2.7 × 10⁸ calc. | pH 8, 37 °C | Open record: exoribonuclease II (RNase II) variant E542A, poly(A) oligoribonucleotide, 35 nt (5'-32P-labelled) |