Values CatLog read from this paper
Creating lactose phosphorylase enzymes by directed evolution of cellobiose phosphorylase
PMID 19487233doi:10.1093/protein/gzp017Open in Browse
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CatLog read 8 records (24 values) from this paper: cellobiose phosphorylase (CP) from Cellulomonas uda.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| cellobiose phosphorylase (CP) | Cellulomonas uda | cellobiose | 13.2 | 2.9 | 4.557 | pH 6.6, 37 °C | Open record: cellobiose phosphorylase (CP), cellobiose |
| cellobiose phosphorylase (CP) | Cellulomonas uda | lactose | 0.109 | 228 | 4.8 × 10⁻⁴ | pH 6.6, 37 °C | Open record: cellobiose phosphorylase (CP), lactose |
| cellobiose phosphorylase (CP)variant A397V/T508A/A512T/D557N/N667T/G681S | Cellulomonas uda | cellobiose | 2.01 | 1.7 | 1.21 | pH 6.6, 37 °C | Open record: cellobiose phosphorylase (CP) variant A397V/T508A/A512T/D557N/N667T/G681S, cellobiose |
| cellobiose phosphorylase (CP)variant A397V/T508A/A512T/D557N/N667T/G681S | Cellulomonas uda | lactose | 0.35 | 230 | 0.00151 | pH 6.6, 37 °C | Open record: cellobiose phosphorylase (CP) variant A397V/T508A/A512T/D557N/N667T/G681S, lactose |
| cellobiose phosphorylase (CP)variant T508A/N667T | Cellulomonas uda | cellobiose | 2.61 | 2.9 | 0.904 | pH 6.6, 37 °C | Open record: cellobiose phosphorylase (CP) variant T508A/N667T, cellobiose |
| cellobiose phosphorylase (CP)variant T508A/N667T | Cellulomonas uda | lactose | 0.53 | 254 | 0.00207 | pH 6.6, 37 °C | Open record: cellobiose phosphorylase (CP) variant T508A/N667T, lactose |
| cellobiose phosphorylase (CP)variant T508I/N667A | Cellulomonas uda | cellobiose | 3.28 | 3.3 | 0.986 | pH 6.6, 37 °C | Open record: cellobiose phosphorylase (CP) variant T508I/N667A, cellobiose |
| cellobiose phosphorylase (CP)variant T508I/N667A | Cellulomonas uda | lactose | 1.11 | 330 | 0.00336 | pH 6.6, 37 °C | Open record: cellobiose phosphorylase (CP) variant T508I/N667A, lactose |