Values CatLog read from this paper
Efficient screening for new amino acid dehydrogenase activity: directed evolution of Bacillus sphaericus phenylalanine dehydrogenase towards activity with an unsaturated non-natural amino acid
PMID 19501264doi:10.1016/j.jbiotec.2009.03.005Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 28 records (84 values) from this paper: phenylalanine dehydrogenase (PheDH) from Bacillus sphaericus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| phenylalanine dehydrogenase (PheDH) | Bacillus sphaericus | DL-propargylglycine | 0.722 | 134 | 0.0054 | Open record: phenylalanine dehydrogenase (PheDH), DL-propargylglycine | |
| phenylalanine dehydrogenase (PheDH) | Bacillus sphaericus | L-phenylalanine | 57.1 | 1.41 | 40.5 | Open record: phenylalanine dehydrogenase (PheDH), L-phenylalanine | |
| phenylalanine dehydrogenase (PheDH)variant D209G (1st A7) | Bacillus sphaericus | DL-propargylglycine | 1.82 | 298 | 0.0061 | Open record: phenylalanine dehydrogenase (PheDH) variant D209G (1st A7), DL-propargylglycine | |
| phenylalanine dehydrogenase (PheDH)variant D209G (1st A7) | Bacillus sphaericus | L-phenylalanine | 56.7 | 0.668 | 84.9 | Open record: phenylalanine dehydrogenase (PheDH) variant D209G (1st A7), L-phenylalanine | |
| phenylalanine dehydrogenase (PheDH)variant E313G (H7H10) | Bacillus sphaericus | DL-propargylglycine | 1.07 | 27.9 | 0.0384 | Open record: phenylalanine dehydrogenase (PheDH) variant E313G (H7H10), DL-propargylglycine | |
| phenylalanine dehydrogenase (PheDH)variant E313G (H7H10) | Bacillus sphaericus | L-phenylalanine | 32.1 | 0.115 | 279 | Open record: phenylalanine dehydrogenase (PheDH) variant E313G (H7H10), L-phenylalanine | |
| phenylalanine dehydrogenase (PheDH)variant F110Y/G124C/G293A (H14A12) | Bacillus sphaericus | DL-propargylglycine | 0.759 | 41.5 | 0.0183 | Open record: phenylalanine dehydrogenase (PheDH) variant F110Y/G124C/G293A (H14A12), DL-propargylglycine | |
| phenylalanine dehydrogenase (PheDH)variant F110Y/G124C/G293A (H14A12) | Bacillus sphaericus | L-phenylalanine | 1.12 | 1.51 | 0.74 | Open record: phenylalanine dehydrogenase (PheDH) variant F110Y/G124C/G293A (H14A12), L-phenylalanine | |
| phenylalanine dehydrogenase (PheDH)variant G124A (9F5) | Bacillus sphaericus | DL-propargylglycine | 0.69 | 27.8 | 0.025 | Open record: phenylalanine dehydrogenase (PheDH) variant G124A (9F5), DL-propargylglycine | |
| phenylalanine dehydrogenase (PheDH)variant G124A (9F5) | Bacillus sphaericus | L-phenylalanine | 2.6 | 7.89 | 0.33 | Open record: phenylalanine dehydrogenase (PheDH) variant G124A (9F5), L-phenylalanine | |
| phenylalanine dehydrogenase (PheDH)variant G124A/E313G (25B12) | Bacillus sphaericus | DL-propargylglycine | 0.92 | 23.2 | 0.04 | Open record: phenylalanine dehydrogenase (PheDH) variant G124A/E313G (25B12), DL-propargylglycine | |
| phenylalanine dehydrogenase (PheDH)variant G124A/E313G (25B12) | Bacillus sphaericus | L-phenylalanine | 4.66 | 9.56 | 0.49 | Open record: phenylalanine dehydrogenase (PheDH) variant G124A/E313G (25B12), L-phenylalanine | |
| phenylalanine dehydrogenase (PheDH)variant G124A/E313N (20E1) | Bacillus sphaericus | DL-propargylglycine | 0.57 | 48.3 | 0.012 | Open record: phenylalanine dehydrogenase (PheDH) variant G124A/E313N (20E1), DL-propargylglycine | |
| phenylalanine dehydrogenase (PheDH)variant G124A/E313N (20E1) | Bacillus sphaericus | L-phenylalanine | 3.07 | 4.5 | 0.68 | Open record: phenylalanine dehydrogenase (PheDH) variant G124A/E313N (20E1), L-phenylalanine | |
| phenylalanine dehydrogenase (PheDH)variant Q18H/I336F (1st B6) | Bacillus sphaericus | DL-propargylglycine | 1.02 | 32.1 | 0.032 | Open record: phenylalanine dehydrogenase (PheDH) variant Q18H/I336F (1st B6), DL-propargylglycine | |
| phenylalanine dehydrogenase (PheDH)variant Q18H/I336F (1st B6) | Bacillus sphaericus | L-phenylalanine | 6.56 | 0.0716 | 91.6 | Open record: phenylalanine dehydrogenase (PheDH) variant Q18H/I336F (1st B6), L-phenylalanine | |
| phenylalanine dehydrogenase (PheDH)variant Q18H/I336F/D209G (1st A7/1st B6) | Bacillus sphaericus | DL-propargylglycine | 1.3 | 58.1 | 0.022 | Open record: phenylalanine dehydrogenase (PheDH) variant Q18H/I336F/D209G (1st A7/1st B6), DL-propargylglycine | |
| phenylalanine dehydrogenase (PheDH)variant Q18H/I336F/D209G (1st A7/1st B6) | Bacillus sphaericus | L-phenylalanine | 4.08 | 0.07 | 58.3 | Open record: phenylalanine dehydrogenase (PheDH) variant Q18H/I336F/D209G (1st A7/1st B6), L-phenylalanine | |
| phenylalanine dehydrogenase (PheDH)variant Q18H/I336F/E313G (1st B6/H7H10) | Bacillus sphaericus | DL-propargylglycine | 0.59 | 39.3 | 0.015 | Open record: phenylalanine dehydrogenase (PheDH) variant Q18H/I336F/E313G (1st B6/H7H10), DL-propargylglycine | |
| phenylalanine dehydrogenase (PheDH)variant Q18H/I336F/E313G (1st B6/H7H10) | Bacillus sphaericus | L-phenylalanine | 0.91 | 0.018 | 50.6 | Open record: phenylalanine dehydrogenase (PheDH) variant Q18H/I336F/E313G (1st B6/H7H10), L-phenylalanine | |
| phenylalanine dehydrogenase (PheDH)variant Q18H/I336F/K224Q/L283F (2nd B10) | Bacillus sphaericus | DL-propargylglycine | 1.15 | 33.6 | 0.034 | Open record: phenylalanine dehydrogenase (PheDH) variant Q18H/I336F/K224Q/L283F (2nd B10), DL-propargylglycine | |
| phenylalanine dehydrogenase (PheDH)variant Q18H/I336F/K224Q/L283F (2nd B10) | Bacillus sphaericus | L-phenylalanine | 3.16 | 0.0462 | 68.4 | Open record: phenylalanine dehydrogenase (PheDH) variant Q18H/I336F/K224Q/L283F (2nd B10), L-phenylalanine | |
| phenylalanine dehydrogenase (PheDH)variant Q18H/I336F/K224Q/L283F/E313G (2nd B10/H7H10) | Bacillus sphaericus | DL-propargylglycine | 0.5 | 32.5 | 0.015 | Open record: phenylalanine dehydrogenase (PheDH) variant Q18H/I336F/K224Q/L283F/E313G (2nd B10/H7H10), DL-propargylglycine | |
| phenylalanine dehydrogenase (PheDH)variant Q18H/I336F/K224Q/L283F/E313G (2nd B10/H7H10) | Bacillus sphaericus | L-phenylalanine | 1.09 | 0.0196 | 55.6 | Open record: phenylalanine dehydrogenase (PheDH) variant Q18H/I336F/K224Q/L283F/E313G (2nd B10/H7H10), L-phenylalanine | |
| phenylalanine dehydrogenase (PheDH)variant Q18H/I336F/V33A/A206D/L283F (2nd C2) | Bacillus sphaericus | DL-propargylglycine | 1.42 | 44.1 | 0.032 | Open record: phenylalanine dehydrogenase (PheDH) variant Q18H/I336F/V33A/A206D/L283F (2nd C2), DL-propargylglycine | |
| phenylalanine dehydrogenase (PheDH)variant Q18H/I336F/V33A/A206D/L283F (2nd C2) | Bacillus sphaericus | L-phenylalanine | 5.8 | 0.0566 | 102.5 | Open record: phenylalanine dehydrogenase (PheDH) variant Q18H/I336F/V33A/A206D/L283F (2nd C2), L-phenylalanine | |
| phenylalanine dehydrogenase (PheDH)variant V135I/I308N/Q363H (H21D1) | Bacillus sphaericus | DL-propargylglycine | 1.49 | 84.5 | 0.0176 | Open record: phenylalanine dehydrogenase (PheDH) variant V135I/I308N/Q363H (H21D1), DL-propargylglycine | |
| phenylalanine dehydrogenase (PheDH)variant V135I/I308N/Q363H (H21D1) | Bacillus sphaericus | L-phenylalanine | 0.608 | 0.0349 | 17.4 | Open record: phenylalanine dehydrogenase (PheDH) variant V135I/I308N/Q363H (H21D1), L-phenylalanine |