Values CatLog read from this paper

Saturation-mutagenesis in two positions distant from active site of a Klebsiella pneumoniae glycerol dehydratase identifies some highly active mutants

PMID 19555728doi:10.1016/j.jbiotec.2009.06.015Open in Browse

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CatLog read 62 records (62 values) from this paper: glycerol dehydratase from Klebsiella pneumoniae.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 4.2.1.30

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
glycerol dehydratase	Klebsiella pneumoniae	1,2-propanediol	—	0.24	—	pH 9, 45 °C	Open record: glycerol dehydratase, 1,2-propanediol
glycerol dehydratase	Klebsiella pneumoniae	glycerol	—	0.6	—	pH 9, 45 °C	Open record: glycerol dehydratase, glycerol
glycerol dehydratasevariant α-I498A	Klebsiella pneumoniae	1,2-propanediol	—	0.95	—	pH 9, 45 °C	Open record: glycerol dehydratase variant α-I498A, 1,2-propanediol
glycerol dehydratasevariant α-I498A	Klebsiella pneumoniae	glycerol	—	0.58	—	pH 9, 45 °C	Open record: glycerol dehydratase variant α-I498A, glycerol
glycerol dehydratasevariant α-I498C	Klebsiella pneumoniae	1,2-propanediol	—	0.24	—	pH 9, 40 °C	Open record: glycerol dehydratase variant α-I498C, 1,2-propanediol
glycerol dehydratasevariant α-I498C	Klebsiella pneumoniae	glycerol	—	0.6	—	pH 9, 40 °C	Open record: glycerol dehydratase variant α-I498C, glycerol
glycerol dehydratasevariant α-I498D	Klebsiella pneumoniae	1,2-propanediol	—	1	—	pH 8, 45 °C	Open record: glycerol dehydratase variant α-I498D, 1,2-propanediol
glycerol dehydratasevariant α-I498D	Klebsiella pneumoniae	glycerol	—	1.5	—	pH 8, 45 °C	Open record: glycerol dehydratase variant α-I498D, glycerol
glycerol dehydratasevariant α-I498M	Klebsiella pneumoniae	1,2-propanediol	—	0.12	—	pH 8, 42 °C	Open record: glycerol dehydratase variant α-I498M, 1,2-propanediol
glycerol dehydratasevariant α-I498M	Klebsiella pneumoniae	glycerol	—	0.5	—	pH 8, 42 °C	Open record: glycerol dehydratase variant α-I498M, glycerol
glycerol dehydratasevariant α-I498N	Klebsiella pneumoniae	1,2-propanediol	—	0.09	—	pH 9, 42 °C	Open record: glycerol dehydratase variant α-I498N, 1,2-propanediol
glycerol dehydratasevariant α-I498N	Klebsiella pneumoniae	glycerol	—	0.55	—	pH 9, 42 °C	Open record: glycerol dehydratase variant α-I498N, glycerol
glycerol dehydratasevariant α-I498P	Klebsiella pneumoniae	1,2-propanediol	—	1.9	—	pH 8, 42 °C	Open record: glycerol dehydratase variant α-I498P, 1,2-propanediol
glycerol dehydratasevariant α-I498P	Klebsiella pneumoniae	glycerol	—	0.9	—	pH 8, 42 °C	Open record: glycerol dehydratase variant α-I498P, glycerol
glycerol dehydratasevariant α-I498S	Klebsiella pneumoniae	1,2-propanediol	—	0.37	—	pH 7.5, 42 °C	Open record: glycerol dehydratase variant α-I498S, 1,2-propanediol
glycerol dehydratasevariant α-I498S	Klebsiella pneumoniae	glycerol	—	0.67	—	pH 7.5, 42 °C	Open record: glycerol dehydratase variant α-I498S, glycerol
glycerol dehydratasevariant α-I498T	Klebsiella pneumoniae	1,2-propanediol	—	3.3	—	pH 8.5, 45 °C	Open record: glycerol dehydratase variant α-I498T, 1,2-propanediol
glycerol dehydratasevariant α-I498T	Klebsiella pneumoniae	glycerol	—	0.52	—	pH 8.5, 45 °C	Open record: glycerol dehydratase variant α-I498T, glycerol
glycerol dehydratasevariant α-I498V	Klebsiella pneumoniae	1,2-propanediol	—	0.17	—	pH 9, 45 °C	Open record: glycerol dehydratase variant α-I498V, 1,2-propanediol
glycerol dehydratasevariant α-I498V	Klebsiella pneumoniae	glycerol	—	0.59	—	pH 9, 45 °C	Open record: glycerol dehydratase variant α-I498V, glycerol
glycerol dehydratasevariant α-I498W	Klebsiella pneumoniae	1,2-propanediol	—	0.19	—	pH 8, 42 °C	Open record: glycerol dehydratase variant α-I498W, 1,2-propanediol
glycerol dehydratasevariant α-I498W	Klebsiella pneumoniae	glycerol	—	0.62	—	pH 8, 42 °C	Open record: glycerol dehydratase variant α-I498W, glycerol
glycerol dehydratasevariant β-Q42A	Klebsiella pneumoniae	1,2-propanediol	—	0.75	—	pH 7.5, 42 °C	Open record: glycerol dehydratase variant β-Q42A, 1,2-propanediol
glycerol dehydratasevariant β-Q42A	Klebsiella pneumoniae	glycerol	—	1.8	—	pH 7.5, 42 °C	Open record: glycerol dehydratase variant β-Q42A, glycerol
glycerol dehydratasevariant β-Q42C	Klebsiella pneumoniae	1,2-propanediol	—	1.13	—	pH 8, 42 °C	Open record: glycerol dehydratase variant β-Q42C, 1,2-propanediol
glycerol dehydratasevariant β-Q42C	Klebsiella pneumoniae	glycerol	—	1.22	—	pH 8, 42 °C	Open record: glycerol dehydratase variant β-Q42C, glycerol
glycerol dehydratasevariant β-Q42D	Klebsiella pneumoniae	1,2-propanediol	—	0.19	—	pH 9.5, 42 °C	Open record: glycerol dehydratase variant β-Q42D, 1,2-propanediol
glycerol dehydratasevariant β-Q42D	Klebsiella pneumoniae	glycerol	—	1.94	—	pH 9.5, 42 °C	Open record: glycerol dehydratase variant β-Q42D, glycerol
glycerol dehydratasevariant β-Q42E	Klebsiella pneumoniae	1,2-propanediol	—	0.19	—	pH 8, 42 °C	Open record: glycerol dehydratase variant β-Q42E, 1,2-propanediol
glycerol dehydratasevariant β-Q42E	Klebsiella pneumoniae	glycerol	—	0.62	—	pH 8, 42 °C	Open record: glycerol dehydratase variant β-Q42E, glycerol
glycerol dehydratasevariant β-Q42F	Klebsiella pneumoniae	1,2-propanediolTable 3	—	0.19	—	pH 9.5, 47 °C	Open record: glycerol dehydratase variant β-Q42F, 1,2-propanediol
glycerol dehydratasevariant β-Q42F	Klebsiella pneumoniae	1,2-propanediolin the text	—	—	3.2 × 10³	pH 9.5, 47 °C	Open record: glycerol dehydratase variant β-Q42F, 1,2-propanediol
glycerol dehydratasevariant β-Q42F	Klebsiella pneumoniae	glycerolTable 3	—	2.61	—	pH 9.5, 47 °C	Open record: glycerol dehydratase variant β-Q42F, glycerol
glycerol dehydratasevariant β-Q42F	Klebsiella pneumoniae	glycerolin the text	—	—	3122	pH 9.5, 47 °C	Open record: glycerol dehydratase variant β-Q42F, glycerol
glycerol dehydratasevariant β-Q42G	Klebsiella pneumoniae	1,2-propanediol	—	0.65	—	pH 7.5, 42 °C	Open record: glycerol dehydratase variant β-Q42G, 1,2-propanediol
glycerol dehydratasevariant β-Q42G	Klebsiella pneumoniae	glycerol	—	0.6	—	pH 7.5, 42 °C	Open record: glycerol dehydratase variant β-Q42G, glycerol
glycerol dehydratasevariant β-Q42H	Klebsiella pneumoniae	1,2-propanediol	—	0.55	—	pH 9, 47 °C	Open record: glycerol dehydratase variant β-Q42H, 1,2-propanediol
glycerol dehydratasevariant β-Q42H	Klebsiella pneumoniae	glycerol	—	0.66	—	pH 9, 47 °C	Open record: glycerol dehydratase variant β-Q42H, glycerol
glycerol dehydratasevariant β-Q42I	Klebsiella pneumoniae	1,2-propanediol	—	0.64	—	pH 8.5, 37 °C	Open record: glycerol dehydratase variant β-Q42I, 1,2-propanediol
glycerol dehydratasevariant β-Q42I	Klebsiella pneumoniae	glycerol	—	1.07	—	pH 8.5, 37 °C	Open record: glycerol dehydratase variant β-Q42I, glycerol
glycerol dehydratasevariant β-Q42K	Klebsiella pneumoniae	1,2-propanediol	—	0.27	—	pH 9.5, 42 °C	Open record: glycerol dehydratase variant β-Q42K, 1,2-propanediol
glycerol dehydratasevariant β-Q42K	Klebsiella pneumoniae	glycerol	—	2.8	—	pH 9.5, 42 °C	Open record: glycerol dehydratase variant β-Q42K, glycerol
glycerol dehydratasevariant β-Q42L	Klebsiella pneumoniae	1,2-propanediol	—	0.77	—	pH 7.5, 42 °C	Open record: glycerol dehydratase variant β-Q42L, 1,2-propanediol
glycerol dehydratasevariant β-Q42L	Klebsiella pneumoniae	glycerol	—	0.4	—	pH 7.5, 42 °C	Open record: glycerol dehydratase variant β-Q42L, glycerol
glycerol dehydratasevariant β-Q42M	Klebsiella pneumoniae	1,2-propanediol	—	0.24	—	pH 7.5, 42 °C	Open record: glycerol dehydratase variant β-Q42M, 1,2-propanediol
glycerol dehydratasevariant β-Q42M	Klebsiella pneumoniae	glycerol	—	1.18	—	pH 7.5, 42 °C	Open record: glycerol dehydratase variant β-Q42M, glycerol
glycerol dehydratasevariant β-Q42N	Klebsiella pneumoniae	1,2-propanediol	—	0.19	—	pH 9.5, 42 °C	Open record: glycerol dehydratase variant β-Q42N, 1,2-propanediol
glycerol dehydratasevariant β-Q42N	Klebsiella pneumoniae	glycerol	—	0.6	—	pH 9.5, 42 °C	Open record: glycerol dehydratase variant β-Q42N, glycerol
glycerol dehydratasevariant β-Q42P	Klebsiella pneumoniae	1,2-propanediol	—	0.31	—	pH 9, 42 °C	Open record: glycerol dehydratase variant β-Q42P, 1,2-propanediol
glycerol dehydratasevariant β-Q42P	Klebsiella pneumoniae	glycerol	—	0.7	—	pH 9, 42 °C	Open record: glycerol dehydratase variant β-Q42P, glycerol
glycerol dehydratasevariant β-Q42R	Klebsiella pneumoniae	1,2-propanediol	—	0.14	—	pH 9.5, 37 °C	Open record: glycerol dehydratase variant β-Q42R, 1,2-propanediol
glycerol dehydratasevariant β-Q42R	Klebsiella pneumoniae	glycerol	—	0.44	—	pH 9.5, 37 °C	Open record: glycerol dehydratase variant β-Q42R, glycerol
glycerol dehydratasevariant β-Q42S	Klebsiella pneumoniae	1,2-propanediol	—	0.45	—	pH 9.5, 46.7 °C	Open record: glycerol dehydratase variant β-Q42S, 1,2-propanediol
glycerol dehydratasevariant β-Q42S	Klebsiella pneumoniae	glycerol	—	0.48	—	pH 9.5, 46.7 °C	Open record: glycerol dehydratase variant β-Q42S, glycerol
glycerol dehydratasevariant β-Q42T	Klebsiella pneumoniae	1,2-propanediol	—	1.38	—	pH 7.5, 42 °C	Open record: glycerol dehydratase variant β-Q42T, 1,2-propanediol
glycerol dehydratasevariant β-Q42T	Klebsiella pneumoniae	glycerol	—	0.82	—	pH 7.5, 42 °C	Open record: glycerol dehydratase variant β-Q42T, glycerol
glycerol dehydratasevariant β-Q42V	Klebsiella pneumoniae	1,2-propanediol	—	1.54	—	pH 7.5, 42 °C	Open record: glycerol dehydratase variant β-Q42V, 1,2-propanediol
glycerol dehydratasevariant β-Q42V	Klebsiella pneumoniae	glycerol	—	1.3	—	pH 7.5, 42 °C	Open record: glycerol dehydratase variant β-Q42V, glycerol
glycerol dehydratasevariant β-Q42W	Klebsiella pneumoniae	1,2-propanediol	—	0.54	—	pH 7.5, 42 °C	Open record: glycerol dehydratase variant β-Q42W, 1,2-propanediol
glycerol dehydratasevariant β-Q42W	Klebsiella pneumoniae	glycerol	—	0.57	—	pH 7.5, 42 °C	Open record: glycerol dehydratase variant β-Q42W, glycerol
glycerol dehydratasevariant β-Q42Y	Klebsiella pneumoniae	1,2-propanediol	—	0.19	—	pH 7.5, 42 °C	Open record: glycerol dehydratase variant β-Q42Y, 1,2-propanediol
glycerol dehydratasevariant β-Q42Y	Klebsiella pneumoniae	glycerol	—	1.03	—	pH 7.5, 42 °C	Open record: glycerol dehydratase variant β-Q42Y, glycerol