Values CatLog read from this paper
The quaternary structure of Escherichia coli N-acetylneuraminate lyase is essential for functional expression
PMID 19646958doi:10.1016/j.bbrc.2009.07.128Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (22 values) from this paper: N-acetylneuraminate lyase (NAL) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 11 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| N-acetylneuraminate lyase (NAL) | Escherichia coli | N-acetylneuraminic acid | 6.75 | 4.1 | 1.6 calc. | pH 7.6, 30 °C | Open record: N-acetylneuraminate lyase (NAL), N-acetylneuraminic acid |
| N-acetylneuraminate lyase (NAL)variant I229D | Escherichia coli | N-acetylneuraminic acid | 4.69 | 5 | 0.94 calc. | pH 7.6, 30 °C | Open record: N-acetylneuraminate lyase (NAL) variant I229D, N-acetylneuraminic acid |
| N-acetylneuraminate lyase (NAL)variant I229N | Escherichia coli | N-acetylneuraminic acid | 7.4 | 3.4 | 2.2 calc. | pH 7.6, 30 °C | Open record: N-acetylneuraminate lyase (NAL) variant I229N, N-acetylneuraminic acid |
| N-acetylneuraminate lyase (NAL)variant I229R | Escherichia coli | N-acetylneuraminic acid | 7.72 | 3.93 | 1.96 calc. | pH 7.6, 30 °C | Open record: N-acetylneuraminate lyase (NAL) variant I229R, N-acetylneuraminic acid |
| N-acetylneuraminate lyase (NAL)variant L171N/I229N | Escherichia coli | N-acetylneuraminic acid | 3.15 | 2.51 | 1.25 calc. | pH 7.6, 30 °C | Open record: N-acetylneuraminate lyase (NAL) variant L171N/I229N, N-acetylneuraminic acid |
| N-acetylneuraminate lyase (NAL)variant L171R | Escherichia coli | N-acetylneuraminic acid | 5.49 | 3.42 | 1.61 calc. | pH 7.6, 30 °C | Open record: N-acetylneuraminate lyase (NAL) variant L171R, N-acetylneuraminic acid |
| N-acetylneuraminate lyase (NAL)variant L199N | Escherichia coli | N-acetylneuraminic acid | 7.94 | 4.3 | 1.8 calc. | pH 7.6, 30 °C | Open record: N-acetylneuraminate lyase (NAL) variant L199N, N-acetylneuraminic acid |
| N-acetylneuraminate lyase (NAL)variant L199N/I229D | Escherichia coli | N-acetylneuraminic acid | 0.29 | 3.2 | 0.091 calc. | pH 7.6, 30 °C | Open record: N-acetylneuraminate lyase (NAL) variant L199N/I229D, N-acetylneuraminic acid |
| N-acetylneuraminate lyase (NAL)variant L199N/I229N | Escherichia coli | N-acetylneuraminic acid | 0.664 | 4.7 | 0.14 calc. | pH 7.6, 30 °C | Open record: N-acetylneuraminate lyase (NAL) variant L199N/I229N, N-acetylneuraminic acid |
| N-acetylneuraminate lyase (NAL)variant L199N/I229R | Escherichia coli | N-acetylneuraminic acid | 8.9 | 4 | 2.2 calc. | pH 7.6, 30 °C | Open record: N-acetylneuraminate lyase (NAL) variant L199N/I229R, N-acetylneuraminic acid |
| N-acetylneuraminate lyase (NAL)variant L199R/I229N | Escherichia coli | N-acetylneuraminic acid | 11.87 | 6.1 | 1.9 calc. | pH 7.6, 30 °C | Open record: N-acetylneuraminate lyase (NAL) variant L199R/I229N, N-acetylneuraminic acid |