Values CatLog read from this paper
Searching sequence space by definably random mutagenesis: improving the catalytic potency of an enzyme
PMID 1967829doi:10.1073/pnas.87.2.696Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 16 records (32 values) from this paper: triose-phosphate isomerase from Gallus gallus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 16 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| triose-phosphate isomerase | Gallus gallus | D-glyceraldehyde 3-phosphate | 8.3 × 10³ | 0.42 | 2.0 × 10⁴ calc. | pH 7.6, 30 °C | Open record: triose-phosphate isomerase, D-glyceraldehyde 3-phosphate |
| triose-phosphate isomerase | Gallus gallus | dihydroxyacetone phosphate | 600 | 0.65 | 920 calc. | pH 7.6, 30 °C | Open record: triose-phosphate isomerase, dihydroxyacetone phosphate |
| triose-phosphate isomerasevariant E165D | Gallus gallus | D-glyceraldehyde 3-phosphate | 4.2 | 0.078 | 54 calc. | pH 7.6, 30 °C | Open record: triose-phosphate isomerase variant E165D, D-glyceraldehyde 3-phosphate |
| triose-phosphate isomerasevariant E165D | Gallus gallus | dihydroxyacetone phosphate | 4.1 | 1.2 | 3.4 calc. | pH 7.6, 30 °C | Open record: triose-phosphate isomerase variant E165D, dihydroxyacetone phosphate |
| triose-phosphate isomerasevariant E165D/E97D | Gallus gallus | D-glyceraldehyde 3-phosphate | 10 | 0.079 | 130 calc. | pH 7.6, 30 °C | Open record: triose-phosphate isomerase variant E165D/E97D, D-glyceraldehyde 3-phosphate |
| triose-phosphate isomerasevariant E165D/E97D | Gallus gallus | dihydroxyacetone phosphate | 5.8 | 0.93 | 6.2 calc. | pH 7.6, 30 °C | Open record: triose-phosphate isomerase variant E165D/E97D, dihydroxyacetone phosphate |
| triose-phosphate isomerasevariant E165D/G10S | Gallus gallus | D-glyceraldehyde 3-phosphate | 14 | 0.18 | 78 calc. | pH 7.6, 30 °C | Open record: triose-phosphate isomerase variant E165D/G10S, D-glyceraldehyde 3-phosphate |
| triose-phosphate isomerasevariant E165D/G10S | Gallus gallus | dihydroxyacetone phosphate | 4.7 | 1.1 | 4.3 calc. | pH 7.6, 30 °C | Open record: triose-phosphate isomerase variant E165D/G10S, dihydroxyacetone phosphate |
| triose-phosphate isomerasevariant E165D/G233R | Gallus gallus | D-glyceraldehyde 3-phosphate | 8.4 | 0.029 | 290 calc. | pH 7.6, 30 °C | Open record: triose-phosphate isomerase variant E165D/G233R, D-glyceraldehyde 3-phosphate |
| triose-phosphate isomerasevariant E165D/G233R | Gallus gallus | dihydroxyacetone phosphate | 6 | 0.33 | 18 calc. | pH 7.6, 30 °C | Open record: triose-phosphate isomerase variant E165D/G233R, dihydroxyacetone phosphate |
| triose-phosphate isomerasevariant E165D/S96P | Gallus gallus | D-glyceraldehyde 3-phosphate | 68 | 0.066 | 1.0 × 10³ calc. | pH 7.6, 30 °C | Open record: triose-phosphate isomerase variant E165D/S96P, D-glyceraldehyde 3-phosphate |
| triose-phosphate isomerasevariant E165D/S96P | Gallus gallus | dihydroxyacetone phosphate | 3.4 | 0.053 | 64 calc. | pH 7.6, 30 °C | Open record: triose-phosphate isomerase variant E165D/S96P, dihydroxyacetone phosphate |
| triose-phosphate isomerasevariant E165D/S96T | Gallus gallus | D-glyceraldehyde 3-phosphate | 17 | 0.1 | 170 calc. | pH 7.6, 30 °C | Open record: triose-phosphate isomerase variant E165D/S96T, D-glyceraldehyde 3-phosphate |
| triose-phosphate isomerasevariant E165D/S96T | Gallus gallus | dihydroxyacetone phosphate | 9.5 | 1.3 | 7.3 calc. | pH 7.6, 30 °C | Open record: triose-phosphate isomerase variant E165D/S96T, dihydroxyacetone phosphate |
| triose-phosphate isomerasevariant E165D/V167D | Gallus gallus | D-glyceraldehyde 3-phosphate | 15 | 0.17 | 88 calc. | pH 7.6, 30 °C | Open record: triose-phosphate isomerase variant E165D/V167D, D-glyceraldehyde 3-phosphate |
| triose-phosphate isomerasevariant E165D/V167D | Gallus gallus | dihydroxyacetone phosphate | 8.4 | 1.7 | 4.9 calc. | pH 7.6, 30 °C | Open record: triose-phosphate isomerase variant E165D/V167D, dihydroxyacetone phosphate |