Values CatLog read from this paper
Optimization of D-amino acid oxidase for low substrate concentrations--towards a cancer enzyme therapy
PMID 19694805doi:10.1111/j.1742-4658.2009.07191.xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 6 records (9 values) from this paper: D-amino acid oxidase (DAAO) from Rhodotorula gracilis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 3 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| D-amino acid oxidase (DAAO) | Rhodotorula gracilis | D-alanine | 330 | 2.6 | 130 calc. | pH 8.5, 15 °C | Open record: D-amino acid oxidase (DAAO), D-alanine |
| D-amino acid oxidase (DAAO) | Rhodotorula gracilis | O2 | — | 1.9 | — | pH 8.5, 15 °C | Open record: D-amino acid oxidase (DAAO), O2 |
| D-amino acid oxidase (DAAO)variant Q144R | Rhodotorula gracilis | D-alanine | 370 | 4.7 | 79 calc. | pH 8.5, 15 °C | Open record: D-amino acid oxidase (DAAO) variant Q144R, D-alanine |
| D-amino acid oxidase (DAAO)variant Q144R | Rhodotorula gracilis | O2 | — | 2 | — | pH 8.5, 15 °C | Open record: D-amino acid oxidase (DAAO) variant Q144R, O2 |
| D-amino acid oxidase (DAAO)variant S19G/S120P/Q144R/K321M/A345V | Rhodotorula gracilis | D-alanine | 140 | 1.8 | 78 calc. | pH 8.5, 15 °C | Open record: D-amino acid oxidase (DAAO) variant S19G/S120P/Q144R/K321M/A345V, D-alanine |
| D-amino acid oxidase (DAAO)variant S19G/S120P/Q144R/K321M/A345V | Rhodotorula gracilis | O2 | — | 0.22 | — | pH 8.5, 15 °C | Open record: D-amino acid oxidase (DAAO) variant S19G/S120P/Q144R/K321M/A345V, O2 |