Values CatLog read from this paper

Structural and mechanistic roles of three consecutive Pro residues of porcine NADH-cytochrome b(5) reductase for the binding of beta-NADH

PMID 19716516doi:10.1016/j.jbiosc.2009.04.008Open in Browse

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CatLog read 26 records (76 values) from this paper: NADH-cytochrome b5 reductase (b5R), soluble domain from Sus scrofa.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.6.2.2

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
NADH-cytochrome b5 reductase (b5R), soluble domain	Sus scrofa	ferricyanide	337	0.00723	4.661 × 10⁴	pH 7, 25 °C	Open record: NADH-cytochrome b5 reductase (b5R), soluble domain, ferricyanide
NADH-cytochrome b5 reductase (b5R), soluble domain	Sus scrofa	NADHNADH:ferricyanide reductase assay; 3.0 ml	742	0.01097	6.764 × 10⁴	pH 7, 25 °C	Open record: NADH-cytochrome b5 reductase (b5R), soluble domain, NADH
NADH-cytochrome b5 reductase (b5R), soluble domain	Sus scrofa	NADHno His-tag (pCPb5R)	—	0.0053	—	pH 7, 25 °C	Open record: NADH-cytochrome b5 reductase (b5R), soluble domain, NADH
NADH-cytochrome b5 reductase (b5R), soluble domain	Sus scrofa	soluble form of human cytochrome b5 with 6xHis-tag sequence (ferric)	167	0.01735	9.63 × 10³	pH 7, 25 °C	Open record: NADH-cytochrome b5 reductase (b5R), soluble domain, soluble form of human cytochrome b5 with 6xHis-tag sequence (ferric)
NADH-cytochrome b5 reductase (b5R), soluble domain	Sus scrofa	soluble form of human cytochrome b5 without 6xHis-tag (ferric)	147	0.01353	1.086 × 10⁴	pH 7, 25 °C	Open record: NADH-cytochrome b5 reductase (b5R), soluble domain, soluble form of human cytochrome b5 without 6xHis-tag (ferric)
NADH-cytochrome b5 reductase (b5R), soluble domainvariant P247A	Sus scrofa	ferricyanide	357	0.01096	3.257 × 10⁴	pH 7, 25 °C	Open record: NADH-cytochrome b5 reductase (b5R), soluble domain variant P247A, ferricyanide
NADH-cytochrome b5 reductase (b5R), soluble domainvariant P247A	Sus scrofa	NADH	354	0.01713	2.067 × 10⁴	pH 7, 25 °C	Open record: NADH-cytochrome b5 reductase (b5R), soluble domain variant P247A, NADH
NADH-cytochrome b5 reductase (b5R), soluble domainvariant P247A	Sus scrofa	soluble form of human cytochrome b5 with 6xHis-tag sequence (ferric)	200	0.02407	8.31 × 10³	pH 7, 25 °C	Open record: NADH-cytochrome b5 reductase (b5R), soluble domain variant P247A, soluble form of human cytochrome b5 with 6xHis-tag sequence (ferric)
NADH-cytochrome b5 reductase (b5R), soluble domainvariant P247A	Sus scrofa	soluble form of human cytochrome b5 without 6xHis-tag (ferric)	167	0.0207	8.07 × 10³	pH 7, 25 °C	Open record: NADH-cytochrome b5 reductase (b5R), soluble domain variant P247A, soluble form of human cytochrome b5 without 6xHis-tag (ferric)
NADH-cytochrome b5 reductase (b5R), soluble domainvariant P247L	Sus scrofa	ferricyanide	30.8	0.00104	2.962 × 10⁴	pH 7, 25 °C	Open record: NADH-cytochrome b5 reductase (b5R), soluble domain variant P247L, ferricyanide
NADH-cytochrome b5 reductase (b5R), soluble domainvariant P247L	Sus scrofa	NADH	21.7	0.02467	880	pH 7, 25 °C	Open record: NADH-cytochrome b5 reductase (b5R), soluble domain variant P247L, NADH
NADH-cytochrome b5 reductase (b5R), soluble domainvariant P247L	Sus scrofa	soluble form of human cytochrome b5 with 6xHis-tag sequence (ferric)	27.2	0.00693	3.92 × 10³	pH 7, 25 °C	Open record: NADH-cytochrome b5 reductase (b5R), soluble domain variant P247L, soluble form of human cytochrome b5 with 6xHis-tag sequence (ferric)
NADH-cytochrome b5 reductase (b5R), soluble domainvariant P248A	Sus scrofa	ferricyanide	399	0.01563	2.553 × 10⁴	pH 7, 25 °C	Open record: NADH-cytochrome b5 reductase (b5R), soluble domain variant P248A, ferricyanide
NADH-cytochrome b5 reductase (b5R), soluble domainvariant P248A	Sus scrofa	NADH	559	0.02407	2.322 × 10⁴	pH 7, 25 °C	Open record: NADH-cytochrome b5 reductase (b5R), soluble domain variant P248A, NADH
NADH-cytochrome b5 reductase (b5R), soluble domainvariant P248A	Sus scrofa	soluble form of human cytochrome b5 with 6xHis-tag sequence (ferric)	288	0.04148	6.94 × 10³	pH 7, 25 °C	Open record: NADH-cytochrome b5 reductase (b5R), soluble domain variant P248A, soluble form of human cytochrome b5 with 6xHis-tag sequence (ferric)
NADH-cytochrome b5 reductase (b5R), soluble domainvariant P248A	Sus scrofa	soluble form of human cytochrome b5 without 6xHis-tag (ferric)	301	0.03434	8.77 × 10³	pH 7, 25 °C	Open record: NADH-cytochrome b5 reductase (b5R), soluble domain variant P248A, soluble form of human cytochrome b5 without 6xHis-tag (ferric)
NADH-cytochrome b5 reductase (b5R), soluble domainvariant P248L	Sus scrofa	ferricyanide	504	0.01568	3.214 × 10⁴	pH 7, 25 °C	Open record: NADH-cytochrome b5 reductase (b5R), soluble domain variant P248L, ferricyanide
NADH-cytochrome b5 reductase (b5R), soluble domainvariant P248L	Sus scrofa	NADH	540	0.0135	4 × 10⁴	pH 7, 25 °C	Open record: NADH-cytochrome b5 reductase (b5R), soluble domain variant P248L, NADH
NADH-cytochrome b5 reductase (b5R), soluble domainvariant P248L	Sus scrofa	soluble form of human cytochrome b5 with 6xHis-tag sequence (ferric)	275	0.08199	3.35 × 10³	pH 7, 25 °C	Open record: NADH-cytochrome b5 reductase (b5R), soluble domain variant P248L, soluble form of human cytochrome b5 with 6xHis-tag sequence (ferric)
NADH-cytochrome b5 reductase (b5R), soluble domainvariant P249A	Sus scrofa	ferricyanide	838	0.02646	3.167 × 10⁴	pH 7, 25 °C	Open record: NADH-cytochrome b5 reductase (b5R), soluble domain variant P249A, ferricyanide
NADH-cytochrome b5 reductase (b5R), soluble domainvariant P249A	Sus scrofa	NADH	681	0.02928	2.326 × 10⁴	pH 7, 25 °C	Open record: NADH-cytochrome b5 reductase (b5R), soluble domain variant P249A, NADH
NADH-cytochrome b5 reductase (b5R), soluble domainvariant P249A	Sus scrofa	soluble form of human cytochrome b5 with 6xHis-tag sequence (ferric)	441	0.05238	8.42 × 10³	pH 7, 25 °C	Open record: NADH-cytochrome b5 reductase (b5R), soluble domain variant P249A, soluble form of human cytochrome b5 with 6xHis-tag sequence (ferric)
NADH-cytochrome b5 reductase (b5R), soluble domainvariant P249A	Sus scrofa	soluble form of human cytochrome b5 without 6xHis-tag (ferric)	540	0.04481	1.205 × 10⁴	pH 7, 25 °C	Open record: NADH-cytochrome b5 reductase (b5R), soluble domain variant P249A, soluble form of human cytochrome b5 without 6xHis-tag (ferric)
NADH-cytochrome b5 reductase (b5R), soluble domainvariant P249L	Sus scrofa	ferricyanide	1241	0.03309	3.75 × 10⁴	pH 7, 25 °C	Open record: NADH-cytochrome b5 reductase (b5R), soluble domain variant P249L, ferricyanide
NADH-cytochrome b5 reductase (b5R), soluble domainvariant P249L	Sus scrofa	NADH	1059	0.0155	6.832 × 10⁴	pH 7, 25 °C	Open record: NADH-cytochrome b5 reductase (b5R), soluble domain variant P249L, NADH
NADH-cytochrome b5 reductase (b5R), soluble domainvariant P249L	Sus scrofa	soluble form of human cytochrome b5 with 6xHis-tag sequence (ferric)	270	0.055	4.91 × 10³	pH 7, 25 °C	Open record: NADH-cytochrome b5 reductase (b5R), soluble domain variant P249L, soluble form of human cytochrome b5 with 6xHis-tag sequence (ferric)