Values CatLog read from this paper
Phenylalanine ammonia-lyase (PAL) from tobacco (Nicotiana tabacum): characterization of the four tobacco PAL genes and active heterotetrameric enzymes
PMID 19725811doi:10.1042/bj20090620Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (28 values) from this paper: PAL1, PAL2, PAL1–PAL2 heterotetramer and 2 more from Nicotiana tabacum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| PAL1 | Nicotiana tabacum | L-phenylalanineN-terminal His-tag (pET15b) removed by thrombin cleavage | 1.09 | 0.0598 | 18.28 | pH 8.5, 30 °C | Open record: PAL1, L-phenylalanine |
| PAL1 | Nicotiana tabacum | L-phenylalanine | 1.12 | 0.0562 | 20 | pH 8.5, 30 °C | Open record: PAL1, L-phenylalanine |
| PAL1 | Nicotiana tabacum | L-phenylalanineC-terminal His-tag (pET29a) | — | — | 18.65 | pH 8.5, 30 °C | Open record: PAL1, L-phenylalanine |
| PAL1–PAL2 heterotetramer | Nicotiana tabacum | L-phenylalanine | 1.26 | 0.051 | 24.68 | pH 8.5, 30 °C | Open record: PAL1–PAL2 heterotetramer, L-phenylalanine |
| PAL1–PAL2 heterotetramervariant G495D in the PAL2 subunit (PAL1–PAL2D495) | Nicotiana tabacum | L-phenylalanine | 0.58 | 0.0465 | 12.47 | pH 8.5, 30 °C | Open record: PAL1–PAL2 heterotetramer variant G495D in the PAL2 subunit (PAL1–PAL2D495), L-phenylalanine |
| PAL2 | Nicotiana tabacum | L-phenylalanineN-terminal His-tag (pET15b) removed by thrombin cleavage | 1.14 | 0.0395 | 28.9 | pH 8.5, 30 °C | Open record: PAL2, L-phenylalanine |
| PAL2 | Nicotiana tabacum | L-phenylalanine | 1.35 | 0.0466 | 28.94 | pH 8.5, 30 °C | Open record: PAL2, L-phenylalanine |
| PAL2 | Nicotiana tabacum | L-phenylalanineC-terminal His-tag (pET29a) | — | — | 21.32 | pH 8.5, 30 °C | Open record: PAL2, L-phenylalanine |
| PAL3 | Nicotiana tabacum | L-phenylalanineN-terminal His-tag (pET15b) removed by thrombin cleavage | 0.78 | 0.0364 | 21.1 | pH 8.5, 30 °C | Open record: PAL3, L-phenylalanine |
| PAL3 | Nicotiana tabacum | L-phenylalanineC-terminal His-tag (pET29a) | — | — | 9.09 | pH 8.5, 30 °C | Open record: PAL3, L-phenylalanine |
| PAL4 | Nicotiana tabacum | L-phenylalanineN-terminal His-tag (pET15b) removed by thrombin cleavage | 1.53 | 0.0524 | 29.09 | pH 8.5, 30 °C | Open record: PAL4, L-phenylalanine |
| PAL4 | Nicotiana tabacum | L-phenylalanineC-terminal His-tag (pET29a) | — | — | 10.29 | pH 8.5, 30 °C | Open record: PAL4, L-phenylalanine |