Values CatLog read from this paper
The role of lysine-234 in beta-lactamase catalysis probed by site-directed mutagenesis
PMID 1974463doi:10.1021/bi00476a022Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 48 records (109 values) from this paper: beta-lactamase from Bacillus licheniformis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 19 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| beta-lactamase | Bacillus licheniformis | 3α-(hydroxymethyl)-2,2-dimethyl-6β-(phenoxyacetamido)penam | 112 | 0.9 | 120 calc. | pH 8, 30 °C | Open record: beta-lactamase, 3α-(hydroxymethyl)-2,2-dimethyl-6β-(phenoxyacetamido)penam |
| beta-lactamase | Bacillus licheniformis | 3α-(hydroxymethyl)-2,2-dimethyl-6β-(phenoxyacetamido)penam | 65 | 0.28 | 230 calc. | pH 9, 30 °C | Open record: beta-lactamase, 3α-(hydroxymethyl)-2,2-dimethyl-6β-(phenoxyacetamido)penam |
| beta-lactamase | Bacillus licheniformis | [2-[[p-(dimethylamino)phenyl]azo]pyridinio]cephalosporin | 667 | 0.14 | 4.63 × 10³ | pH 7, 30 °C | Open record: beta-lactamase, [2-[[p-(dimethylamino)phenyl]azo]pyridinio]cephalosporin |
| beta-lactamase | Bacillus licheniformis | ampicillin | 2241 | 0.21 | 1.06 × 10⁴ | pH 7, 30 °C | Open record: beta-lactamase, ampicillin |
| beta-lactamase | Bacillus licheniformis | benzylpenicillin | 2.65 × 10³ | 0.12 | 2.15 × 10⁴ | pH 7, 30 °C | Open record: beta-lactamase, benzylpenicillin |
| beta-lactamase | Bacillus licheniformis | nitrocefin | 1088 | 0.041 | 2.64 × 10⁴ | pH 7, 30 °C | Open record: beta-lactamase, nitrocefin |
| beta-lactamase | Bacillus licheniformis | phenoxymethylpenicillin50 mM sodium phosphate; 0.15 M KCl | 2552 | 0.1 | 2.48 × 10⁴ | pH 7, 30 °C | Open record: beta-lactamase, phenoxymethylpenicillin |
| beta-lactamase | Bacillus licheniformis | phenoxymethylpenicillin50 mM sodium phosphate; 0 M KCl | 1936 | 0.042 | 4.6 × 10⁴ | pH 7, 30 °C | Open record: beta-lactamase, phenoxymethylpenicillin |
| beta-lactamase | Bacillus licheniformis | phenoxymethylpenicillin50 mM sodium phosphate; 0.5 M KCl | 2.42 × 10³ | 0.1 | 2.4 × 10⁴ | pH 7, 30 °C | Open record: beta-lactamase, phenoxymethylpenicillin |
| beta-lactamase | Bacillus licheniformis | phenoxymethylpenicillin50 mM sodium phosphate; 1.0 M KCl | 2.6 × 10³ | 0.1 | 2.6 × 10⁴ | pH 7, 30 °C | Open record: beta-lactamase, phenoxymethylpenicillin |
| beta-lactamase | Bacillus licheniformis | phenoxymethylpenicillin50 mM sodium phosphate; 1.5 M KCl | 2.45 × 10³ | 0.1 | 2.4 × 10⁴ | pH 7, 30 °C | Open record: beta-lactamase, phenoxymethylpenicillin |
| beta-lactamase | Bacillus licheniformis | phenoxymethylpenicillin | 1165 | 0.39 | 3.0 × 10³ calc. | pH 4, 30 °C | Open record: beta-lactamase, phenoxymethylpenicillin |
| beta-lactamase | Bacillus licheniformis | phenoxymethylpenicillin | 2.4 × 10³ | 0.32 | 7.5 × 10³ calc. | pH 5, 30 °C | Open record: beta-lactamase, phenoxymethylpenicillin |
| beta-lactamase | Bacillus licheniformis | phenoxymethylpenicillin | 2.7 × 10³ | 0.22 | 1.2 × 10⁴ calc. | pH 6, 30 °C | Open record: beta-lactamase, phenoxymethylpenicillin |
| beta-lactamase | Bacillus licheniformis | phenoxymethylpenicillin40 mM buffer; 0.4 M KCl; 20% methanol | 2.7 × 10³ | 0.16 | 1.7 × 10⁴ calc. | pH 7, 30 °C | Open record: beta-lactamase, phenoxymethylpenicillin |
| beta-lactamase | Bacillus licheniformis | phenoxymethylpenicillin | 1.65 × 10³ | 0.13 | 1.3 × 10⁴ calc. | pH 8, 30 °C | Open record: beta-lactamase, phenoxymethylpenicillin |
| beta-lactamase | Bacillus licheniformis | phenoxymethylpenicillin | 415 | 0.08 | 5.2 × 10³ calc. | pH 9, 30 °C | Open record: beta-lactamase, phenoxymethylpenicillin |
| beta-lactamase | Bacillus licheniformis | phenoxymethylpenicillin | — | — | 2.2 × 10⁴ | 30 °C | Open record: beta-lactamase, phenoxymethylpenicillin |
| beta-lactamasevariant K234A | Bacillus licheniformis | 3α-(hydroxymethyl)-2,2-dimethyl-6β-(phenoxyacetamido)penam | 8 | 0.9 | 8.9 calc. | pH 5, 30 °C | Open record: beta-lactamase variant K234A, 3α-(hydroxymethyl)-2,2-dimethyl-6β-(phenoxyacetamido)penam |
| beta-lactamasevariant K234A | Bacillus licheniformis | [2-[[p-(dimethylamino)phenyl]azo]pyridinio]cephalosporin | — | — | 0.32 | pH 7, 30 °C | Open record: beta-lactamase variant K234A, [2-[[p-(dimethylamino)phenyl]azo]pyridinio]cephalosporin |
| beta-lactamasevariant K234A | Bacillus licheniformis | ampicillin | — | — | 0.89 | pH 7, 30 °C | Open record: beta-lactamase variant K234A, ampicillin |
| beta-lactamasevariant K234A | Bacillus licheniformis | benzylpenicillin | — | — | 2.3 | pH 7, 30 °C | Open record: beta-lactamase variant K234A, benzylpenicillin |
| beta-lactamasevariant K234A | Bacillus licheniformis | nitrocefin | — | — | 3.9 | pH 7, 30 °C | Open record: beta-lactamase variant K234A, nitrocefin |
| beta-lactamasevariant K234A | Bacillus licheniformis | phenoxymethylpenicillin0.15 M KCl | — | — | 9.3 | pH 7, 30 °C | Open record: beta-lactamase variant K234A, phenoxymethylpenicillin |
| beta-lactamasevariant K234A | Bacillus licheniformis | phenoxymethylpenicillin | 0.7 | 0.12 | 5.8 calc. | pH 4, 30 °C | Open record: beta-lactamase variant K234A, phenoxymethylpenicillin |
| beta-lactamasevariant K234A | Bacillus licheniformis | phenoxymethylpenicillin | 95 | 6 | 16 calc. | pH 8.5, 30 °C | Open record: beta-lactamase variant K234A, phenoxymethylpenicillin |
| beta-lactamasevariant K234A | Bacillus licheniformis | phenoxymethylpenicillin0.5 M KCl | 32.2 | 2.8 | 12 | pH 7, 30 °C | Open record: beta-lactamase variant K234A, phenoxymethylpenicillin |
| beta-lactamasevariant K234A | Bacillus licheniformis | phenoxymethylpenicillin1.5 M KCl | 30.3 | 1 | 30 | pH 7, 30 °C | Open record: beta-lactamase variant K234A, phenoxymethylpenicillin |
| beta-lactamasevariant K234A | Bacillus licheniformis | phenoxymethylpenicillin | 1.2 | 0.13 | 9.2 calc. | pH 5, 30 °C | Open record: beta-lactamase variant K234A, phenoxymethylpenicillin |
| beta-lactamasevariant K234A | Bacillus licheniformis | phenoxymethylpenicillin | — | — | 14 | 30 °C | Open record: beta-lactamase variant K234A, phenoxymethylpenicillin |
| beta-lactamasevariant K234E | Bacillus licheniformis | 3α-(hydroxymethyl)-2,2-dimethyl-6β-(phenoxyacetamido)penam | 0.5 | 0.2 | 2.5 calc. | pH 4, 30 °C | Open record: beta-lactamase variant K234E, 3α-(hydroxymethyl)-2,2-dimethyl-6β-(phenoxyacetamido)penam |
| beta-lactamasevariant K234E | Bacillus licheniformis | 3α-(hydroxymethyl)-2,2-dimethyl-6β-(phenoxyacetamido)penam | 4.1 | 0.7 | 5.9 calc. | pH 5, 30 °C | Open record: beta-lactamase variant K234E, 3α-(hydroxymethyl)-2,2-dimethyl-6β-(phenoxyacetamido)penam |
| beta-lactamasevariant K234E | Bacillus licheniformis | [2-[[p-(dimethylamino)phenyl]azo]pyridinio]cephalosporin | 3.2 | 1.2 | 2.7 | pH 7, 30 °C | Open record: beta-lactamase variant K234E, [2-[[p-(dimethylamino)phenyl]azo]pyridinio]cephalosporin |
| beta-lactamasevariant K234E | Bacillus licheniformis | ampicillin | 21.1 | 13.7 | 1.5 | pH 7, 30 °C | Open record: beta-lactamase variant K234E, ampicillin |
| beta-lactamasevariant K234E | Bacillus licheniformis | benzylpenicillin | 49.6 | 8.1 | 6.1 | pH 7, 30 °C | Open record: beta-lactamase variant K234E, benzylpenicillin |
| beta-lactamasevariant K234E | Bacillus licheniformis | nitrocefin | 24.7 | 3.3 | 7.6 | pH 7, 30 °C | Open record: beta-lactamase variant K234E, nitrocefin |
| beta-lactamasevariant K234E | Bacillus licheniformis | phenoxymethylpenicillin0.15 M KCl | 31.7 | 1.5 | 21 | pH 7, 30 °C | Open record: beta-lactamase variant K234E, phenoxymethylpenicillin |
| beta-lactamasevariant K234E | Bacillus licheniformis | phenoxymethylpenicillinKCl | 1.6 | 0.12 | 13 calc. | pH 4, 30 °C | Open record: beta-lactamase variant K234E, phenoxymethylpenicillin |
| beta-lactamasevariant K234E | Bacillus licheniformis | phenoxymethylpenicillin | 33 | 1.9 | 17 calc. | pH 8.5, 30 °C | Open record: beta-lactamase variant K234E, phenoxymethylpenicillin |
| beta-lactamasevariant K234E | Bacillus licheniformis | phenoxymethylpenicillin0 M KCl | 29 | 3 | 9.7 | pH 7, 30 °C | Open record: beta-lactamase variant K234E, phenoxymethylpenicillin |
| beta-lactamasevariant K234E | Bacillus licheniformis | phenoxymethylpenicillin0.3 M KCl | 37.3 | 1.2 | 31 | pH 7, 30 °C | Open record: beta-lactamase variant K234E, phenoxymethylpenicillin |
| beta-lactamasevariant K234E | Bacillus licheniformis | phenoxymethylpenicillin0.5 M KCl | 31 | 0.8 | 39 | pH 7, 30 °C | Open record: beta-lactamase variant K234E, phenoxymethylpenicillin |
| beta-lactamasevariant K234E | Bacillus licheniformis | phenoxymethylpenicillin1.0 M KCl | 27.8 | 0.4 | 69 | pH 7, 30 °C | Open record: beta-lactamase variant K234E, phenoxymethylpenicillin |
| beta-lactamasevariant K234E | Bacillus licheniformis | phenoxymethylpenicillin1.5 M KCl | 32 | 0.3 | 110 | pH 7, 30 °C | Open record: beta-lactamase variant K234E, phenoxymethylpenicillin |
| beta-lactamasevariant K234E | Bacillus licheniformis | phenoxymethylpenicillinKCl (Table III conditions; no methanol) | 1.5 | 0.1 | 15 calc. | pH 4, 30 °C | Open record: beta-lactamase variant K234E, phenoxymethylpenicillin |
| beta-lactamasevariant K234E | Bacillus licheniformis | phenoxymethylpenicillin | 2.3 | 0.15 | 15 calc. | pH 5, 30 °C | Open record: beta-lactamase variant K234E, phenoxymethylpenicillin |
| beta-lactamasevariant K234E | Bacillus licheniformis | phenoxymethylpenicillin | 7 | 0.26 | 27 calc. | pH 6, 30 °C | Open record: beta-lactamase variant K234E, phenoxymethylpenicillin |
| beta-lactamasevariant K234E | Bacillus licheniformis | phenoxymethylpenicillin | — | — | 34 | 30 °C | Open record: beta-lactamase variant K234E, phenoxymethylpenicillin |