Values CatLog read from this paper

Site-directed mutagenesis of beta-lactamase I. Single and double mutants of Glu-166 and Lys-73

PMID 1980064doi:10.1042/bj2720613Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 28 records (82 values) from this paper: beta-lactamase I from Bacillus cereus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.5.2.6

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
beta-lactamase I	Bacillus cereus	6-aminopenicillanic acid	260	1.6	170	pH 7, 20 °C	Open record: beta-lactamase I, 6-aminopenicillanic acid
beta-lactamase I	Bacillus cereus	ampicillin	5 × 10³	0.25	2 × 10⁴	pH 7, 20 °C	Open record: beta-lactamase I, ampicillin
beta-lactamase I	Bacillus cereus	carbenicillin	440	0.15	2.8 × 10³	pH 7, 20 °C	Open record: beta-lactamase I, carbenicillin
beta-lactamase I	Bacillus cereus	cephalosporin C	0.2	0.22	0.9	pH 7, 20 °C	Open record: beta-lactamase I, cephalosporin C
beta-lactamase I	Bacillus cereus	N-(2-furyl)acryloylpenicillin	1.7 × 10³	0.035	4.9 × 10⁴	pH 7, 20 °C	Open record: beta-lactamase I, N-(2-furyl)acryloylpenicillin
beta-lactamase I	Bacillus cereus	nitrocefin	34	0.055	618	pH 7, 20 °C	Open record: beta-lactamase I, nitrocefin
beta-lactamase Ivariant E166D	Bacillus cereus	6-aminopenicillanic acid	1.8	0.81	2.2	pH 7, 20 °C	Open record: beta-lactamase I variant E166D, 6-aminopenicillanic acid
beta-lactamase Ivariant E166D	Bacillus cereus	ampicillin	140	0.46	308	pH 7, 20 °C	Open record: beta-lactamase I variant E166D, ampicillin
beta-lactamase Ivariant E166D	Bacillus cereus	benzylpenicillin	0.67	0.0086	77	pH 7, 20 °C	Open record: beta-lactamase I variant E166D, benzylpenicillin
beta-lactamase Ivariant E166D	Bacillus cereus	carbenicillin	24	0.28	86	pH 7, 20 °C	Open record: beta-lactamase I variant E166D, carbenicillin
beta-lactamase Ivariant E166D	Bacillus cereus	cephalosporin C	0.011	0.38	0.028	pH 7, 20 °C	Open record: beta-lactamase I variant E166D, cephalosporin C
beta-lactamase Ivariant E166D	Bacillus cereus	N-(2-furyl)acryloylpenicillin	30	0.086	343	pH 7, 20 °C	Open record: beta-lactamase I variant E166D, N-(2-furyl)acryloylpenicillin
beta-lactamase Ivariant E166D	Bacillus cereus	nitrocefin	0.81	0.052	16	pH 7, 20 °C	Open record: beta-lactamase I variant E166D, nitrocefin
beta-lactamase Ivariant K73R	Bacillus cereus	6-aminopenicillanic acid	0.049	0.12	0.4	pH 7, 20 °C	Open record: beta-lactamase I variant K73R, 6-aminopenicillanic acid
beta-lactamase Ivariant K73R	Bacillus cereus	ampicillin	1.3	0.082	16	pH 7, 20 °C	Open record: beta-lactamase I variant K73R, ampicillin
beta-lactamase Ivariant K73R	Bacillus cereus	benzylpenicillin	55	0.101	550	pH 7, 20 °C	Open record: beta-lactamase I variant K73R, benzylpenicillin
beta-lactamase Ivariant K73R	Bacillus cereus	carbenicillin	0.46	0.046	10	pH 7, 20 °C	Open record: beta-lactamase I variant K73R, carbenicillin
beta-lactamase Ivariant K73R	Bacillus cereus	cephalosporin C0.1 M sodium phosphate buffer; 0.5 M NaCl; 0.01 mM EDTA	0.001	0.27	0.005	pH 7, 20 °C	Open record: beta-lactamase I variant K73R, cephalosporin C
beta-lactamase Ivariant K73R	Bacillus cereus	cephalosporin Cburst progress curves fitted to eqn (3); followed at 260 nm	—	—	2.7	pH 7, 20 °C	Open record: beta-lactamase I variant K73R, cephalosporin C
beta-lactamase Ivariant K73R	Bacillus cereus	N-(2-furyl)acryloylpenicillin	0.2	0.0068	30	pH 7, 20 °C	Open record: beta-lactamase I variant K73R, N-(2-furyl)acryloylpenicillin
beta-lactamase Ivariant K73R	Bacillus cereus	nitrocefin	0.21	0.078	2.7	pH 7, 20 °C	Open record: beta-lactamase I variant K73R, nitrocefin
beta-lactamase Ivariant K73R/E166D	Bacillus cereus	6-aminopenicillanic acid	0.001	0.16	0.006	pH 7, 20 °C	Open record: beta-lactamase I variant K73R/E166D, 6-aminopenicillanic acid
beta-lactamase Ivariant K73R/E166D	Bacillus cereus	ampicillin	1.4	0.18	8	pH 7, 20 °C	Open record: beta-lactamase I variant K73R/E166D, ampicillin
beta-lactamase Ivariant K73R/E166D	Bacillus cereus	benzylpenicillin	0.47	0.01	47	pH 7, 20 °C	Open record: beta-lactamase I variant K73R/E166D, benzylpenicillin
beta-lactamase Ivariant K73R/E166D	Bacillus cereus	carbenicillin	0.37	0.11	3.3	pH 7, 20 °C	Open record: beta-lactamase I variant K73R/E166D, carbenicillin
beta-lactamase Ivariant K73R/E166D	Bacillus cereus	cephalosporin C	4 × 10⁻⁴	0.18	0.002	pH 7, 20 °C	Open record: beta-lactamase I variant K73R/E166D, cephalosporin C
beta-lactamase Ivariant K73R/E166D	Bacillus cereus	N-(2-furyl)acryloylpenicillin	0.24	0.004	55	pH 7, 20 °C	Open record: beta-lactamase I variant K73R/E166D, N-(2-furyl)acryloylpenicillin
beta-lactamase Ivariant K73R/E166D	Bacillus cereus	nitrocefin	7 × 10⁻⁴	0.0015	0.4	pH 7, 20 °C	Open record: beta-lactamase I variant K73R/E166D, nitrocefin