Values CatLog read from this paper
The oxyanion hole of Pseudomonas fluorescens mannitol 2-dehydrogenase: a novel structural motif for electrostatic stabilization in alcohol dehydrogenase active sites
PMID 19857201doi:10.1042/bj20091441Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 16 records (37 values) from this paper: mannitol 2-dehydrogenase (PfM2DH) from Pseudomonas fluorescens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| mannitol 2-dehydrogenase (PfM2DH) | Pseudomonas fluorescens | D-fructose | 61 | 0.24 | 250 | pH 7.1, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH), D-fructose |
| mannitol 2-dehydrogenase (PfM2DH) | Pseudomonas fluorescens | NADH | — | 0.067 | 910 | pH 7.1, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH), NADH |
| mannitol 2-dehydrogenase (PfM2DH)variant N191A | Pseudomonas fluorescens | D-fructose | 0.56 | 9 | 0.0642 | pH 7.1, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N191A, D-fructose |
| mannitol 2-dehydrogenase (PfM2DH)variant N191A | Pseudomonas fluorescens | D-mannitol | 2.78 | 8.7 | 0.319 | pH 10, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N191A, D-mannitol |
| mannitol 2-dehydrogenase (PfM2DH)variant N191A | Pseudomonas fluorescens | NAD+ | — | 0.31 | 8.968 | pH 10, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N191A, NAD+ |
| mannitol 2-dehydrogenase (PfM2DH)variant N191A | Pseudomonas fluorescens | NADH | — | 0.017 | 32 | pH 7.1, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N191A, NADH |
| mannitol 2-dehydrogenase (PfM2DH)variant N191A/N300A | Pseudomonas fluorescens | D-fructose | — | — | 2.6 × 10⁻⁵ | pH 7.1, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N191A/N300A, D-fructose |
| mannitol 2-dehydrogenase (PfM2DH)variant N191A/N300A | Pseudomonas fluorescens | D-mannitol | 0.04 | 1187 | 3.4 × 10⁻⁵ | pH 10, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N191A/N300A, D-mannitol |
| mannitol 2-dehydrogenase (PfM2DH)variant N191A/N300A | Pseudomonas fluorescens | NAD+ | — | 0.314 | 0.1274 | pH 10, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N191A/N300A, NAD+ |
| mannitol 2-dehydrogenase (PfM2DH)variant N191A/N300A | Pseudomonas fluorescens | NADH | — | 0.023 | — | pH 7.1, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N191A/N300A, NADH |
| mannitol 2-dehydrogenase (PfM2DH)variant N191L | Pseudomonas fluorescens | D-fructose | 0.55 | 1.1 | 0.4074 | pH 7.1, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N191L, D-fructose |
| mannitol 2-dehydrogenase (PfM2DH)variant N191L | Pseudomonas fluorescens | D-mannitol | 0.55 | 0.9 | 0.598 | pH 10, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N191L, D-mannitol |
| mannitol 2-dehydrogenase (PfM2DH)variant N191L | Pseudomonas fluorescens | NAD+ | — | 0.055 | 9.964 | pH 10, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N191L, NAD+ |
| mannitol 2-dehydrogenase (PfM2DH)variant N191L | Pseudomonas fluorescens | NADH | — | 0.0033 | 170 | pH 7.1, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N191L, NADH |
| mannitol 2-dehydrogenase (PfM2DH)variant N300A | Pseudomonas fluorescens | D-fructose | 0.45 | 4.2 | 0.1084 | pH 7.1, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N300A, D-fructose |
| mannitol 2-dehydrogenase (PfM2DH)variant N300A | Pseudomonas fluorescens | NADH | — | 0.0125 | 36 | pH 7.1, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N300A, NADH |