Values CatLog read from this paper

Crystal structure and computational analyses provide insights into the catalytic mechanism of 2,4-diacetylphloroglucinol hydrolase PhlG from Pseudomonas fluorescens

PMID 20018877doi:10.1074/jbc.m109.044180Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 9 records (27 values) from this paper: 2,4-diacetylphloroglucinol hydrolase PhlG from Pseudomonas fluorescens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.7.1.24

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
2,4-diacetylphloroglucinol hydrolase PhlG	Pseudomonas fluorescens	2,4-diacetylphloroglucinol	4.7	0.272	17	pH 7.1, 25 °C	Open record: 2,4-diacetylphloroglucinol hydrolase PhlG, 2,4-diacetylphloroglucinol
2,4-diacetylphloroglucinol hydrolase PhlGvariant E160A	Pseudomonas fluorescens	2,4-diacetylphloroglucinol	8.1 × 10⁻⁵	0.041	0.002	pH 7.1, 25 °C	Open record: 2,4-diacetylphloroglucinol hydrolase PhlG variant E160A, 2,4-diacetylphloroglucinol
2,4-diacetylphloroglucinol hydrolase PhlGvariant E274A	Pseudomonas fluorescens	2,4-diacetylphloroglucinol	2.6 × 10⁻⁵	0.042	6.2 × 10⁻⁴	pH 7.1, 25 °C	Open record: 2,4-diacetylphloroglucinol hydrolase PhlG variant E274A, 2,4-diacetylphloroglucinol
2,4-diacetylphloroglucinol hydrolase PhlGvariant H214A	Pseudomonas fluorescens	2,4-diacetylphloroglucinol	1.4	0.32	4.3	pH 7.1, 25 °C	Open record: 2,4-diacetylphloroglucinol hydrolase PhlG variant H214A, 2,4-diacetylphloroglucinol
2,4-diacetylphloroglucinol hydrolase PhlGvariant H214Q	Pseudomonas fluorescens	2,4-diacetylphloroglucinol	0.58	0.18	3.3	pH 7.1, 25 °C	Open record: 2,4-diacetylphloroglucinol hydrolase PhlG variant H214Q, 2,4-diacetylphloroglucinol
2,4-diacetylphloroglucinol hydrolase PhlGvariant N132A	Pseudomonas fluorescens	2,4-diacetylphloroglucinol	0.74	0.61	1.2	pH 7.1, 25 °C	Open record: 2,4-diacetylphloroglucinol hydrolase PhlG variant N132A, 2,4-diacetylphloroglucinol
2,4-diacetylphloroglucinol hydrolase PhlGvariant Y121A	Pseudomonas fluorescens	2,4-diacetylphloroglucinol	0.05	0.242	0.21	pH 7.1, 25 °C	Open record: 2,4-diacetylphloroglucinol hydrolase PhlG variant Y121A, 2,4-diacetylphloroglucinol
2,4-diacetylphloroglucinol hydrolase PhlGvariant Y229A	Pseudomonas fluorescens	2,4-diacetylphloroglucinol	0.21	0.873	1.1	pH 7.1, 25 °C	Open record: 2,4-diacetylphloroglucinol hydrolase PhlG variant Y229A, 2,4-diacetylphloroglucinol
2,4-diacetylphloroglucinol hydrolase PhlGvariant Y229F	Pseudomonas fluorescens	2,4-diacetylphloroglucinol	1.4	0.268	5.2	pH 7.1, 25 °C	Open record: 2,4-diacetylphloroglucinol hydrolase PhlG variant Y229F, 2,4-diacetylphloroglucinol