Values CatLog read from this paper
Substitution of Glu122 by glutamine revealed the function of the second water molecule as a proton donor in the binuclear metal enzyme creatininase
PMID 20043918doi:10.1016/j.jmb.2009.12.045Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 13 records (39 values) from this paper: creatininase (creatinine amidohydrolase) from Pseudomonas putida.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| creatininase (creatinine amidohydrolase) | Pseudomonas putida | creatineTable 1 | 252 | 44 | 5.73 | Open record: creatininase (creatinine amidohydrolase), creatine | |
| creatininase (creatinine amidohydrolase) | Pseudomonas putida | creatineTable 1 | 1.34 × 10³ | 77 | 17.4 | Open record: creatininase (creatinine amidohydrolase), creatine | |
| creatininase (creatinine amidohydrolase) | Pseudomonas putida | creatineTable 1 | 222 | 150 | 1.48 | Open record: creatininase (creatinine amidohydrolase), creatine | |
| creatininase (creatinine amidohydrolase) | Pseudomonas putida | creatinewild-type enzyme (Zn-Zn) as purified with ZnCl2; Table 2 | 264 | 46 | 5.74 | Open record: creatininase (creatinine amidohydrolase), creatine | |
| creatininase (creatinine amidohydrolase) | Pseudomonas putida | creatinewild-type enzyme (Zn-Zn) as purified with ZnCl2; Table 2 | 437 | 46 | 9.5 | Open record: creatininase (creatinine amidohydrolase), creatine | |
| creatininase (creatinine amidohydrolase) | Pseudomonas putida | creatinesemi-apoenzyme (Zn) purified with 10 mM EDTA in all buffers | 280 | 32 | 8.75 | Open record: creatininase (creatinine amidohydrolase), creatine | |
| creatininase (creatinine amidohydrolase) | Pseudomonas putida | creatinesemi-apoenzyme (Zn) purified with 10 mM EDTA in all buffers | 1.38 × 10³ | 70 | 19.7 | Open record: creatininase (creatinine amidohydrolase), creatine | |
| creatininase (creatinine amidohydrolase)variant E122Q | Pseudomonas putida | creatineTable 1 | 1.5 | 220 | 0.008 | Open record: creatininase (creatinine amidohydrolase) variant E122Q, creatine | |
| creatininase (creatinine amidohydrolase)variant E122Q | Pseudomonas putida | creatineE122Q mutant as purified; Table 2 | 2.9 | 77 | 0.04 | Open record: creatininase (creatinine amidohydrolase) variant E122Q, creatine | |
| creatininase (creatinine amidohydrolase)variant E122Q | Pseudomonas putida | creatineE122Q mutant as purified; Table 2 | 11 | 56 | 0.2 | Open record: creatininase (creatinine amidohydrolase) variant E122Q, creatine | |
| creatininase (creatinine amidohydrolase)variant W154F | Pseudomonas putida | creatine | 18 | 350 | 0.051 | Open record: creatininase (creatinine amidohydrolase) variant W154F, creatine | |
| creatininase (creatinine amidohydrolase)variant W174F | Pseudomonas putida | creatine | 688 | 90 | 7.64 | Open record: creatininase (creatinine amidohydrolase) variant W174F, creatine | |
| creatininase (creatinine amidohydrolase)variant Y121A | Pseudomonas putida | creatine | 86 | 160 | 0.54 | Open record: creatininase (creatinine amidohydrolase) variant Y121A, creatine |