Values CatLog read from this paper
The apparent malate synthase activity of Rhodobacter sphaeroides is due to two paralogous enzymes, (3S)-Malyl-coenzyme A (CoA)/β-methylmalyl-CoA lyase and (3S)- Malyl-CoA thioesterase
PMID 20047909doi:10.1128/jb.01267-09Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 7 records (17 values) from this paper: (3S)-malyl-CoA/β-methylmalyl-CoA lyase (Mcl1), (3S)-malyl-CoA thioesterase (Mcl2) from Rhodobacter sphaeroides.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
EC 3.1.2.30, EC 4.1.3.24, EC 4.1.3.25
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| (3S)-malyl-CoA thioesterase (Mcl2) | Rhodobacter sphaeroides | (3S)-malyl-coenzyme A | 110 | 0.09 | 1.2 × 10³ | pH 7.5, 30 °C | Open record: (3S)-malyl-CoA thioesterase (Mcl2), (3S)-malyl-coenzyme A |
| (3S)-malyl-CoA/β-methylmalyl-CoA lyase (Mcl1) | Rhodobacter sphaeroides | (3S)-malyl-coenzyme A | 2.5 | 0.02 | 130 | pH 7.5, 30 °C | Open record: (3S)-malyl-CoA/β-methylmalyl-CoA lyase (Mcl1), (3S)-malyl-coenzyme A |
| (3S)-malyl-CoA/β-methylmalyl-CoA lyase (Mcl1) | Rhodobacter sphaeroides | acetyl coenzyme A | — | 0.1 | — | pH 7.5, 30 °C | Open record: (3S)-malyl-CoA/β-methylmalyl-CoA lyase (Mcl1), acetyl coenzyme A |
| (3S)-malyl-CoA/β-methylmalyl-CoA lyase (Mcl1) | Rhodobacter sphaeroides | erythro-β-methylmalyl-coenzyme A | 2.8 | 0.01 | 280 | pH 7.5, 30 °C | Open record: (3S)-malyl-CoA/β-methylmalyl-CoA lyase (Mcl1), erythro-β-methylmalyl-coenzyme A |
| (3S)-malyl-CoA/β-methylmalyl-CoA lyase (Mcl1) | Rhodobacter sphaeroides | glyoxylatecosubstrate acetyl-CoA | 8.6 | 3.1 | 2.8 | pH 7.5, 30 °C | Open record: (3S)-malyl-CoA/β-methylmalyl-CoA lyase (Mcl1), glyoxylate |
| (3S)-malyl-CoA/β-methylmalyl-CoA lyase (Mcl1) | Rhodobacter sphaeroides | glyoxylatecosubstrate propionyl-CoA | 12 | 4.1 | 3 | pH 7.5, 30 °C | Open record: (3S)-malyl-CoA/β-methylmalyl-CoA lyase (Mcl1), glyoxylate |
| (3S)-malyl-CoA/β-methylmalyl-CoA lyase (Mcl1) | Rhodobacter sphaeroides | propionyl coenzyme A | — | 0.2 | — | pH 7.5, 30 °C | Open record: (3S)-malyl-CoA/β-methylmalyl-CoA lyase (Mcl1), propionyl coenzyme A |