Values CatLog read from this paper
Structural determinants of substrate recognition in the HAD superfamily member D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB)
PMID 20050614doi:10.1021/bi902019qOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (31 values) from this paper: D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB) from Escherichia coli, Bordetella bronchiseptica.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB) | Bordetella bronchiseptica | D-fructose 1,6-bisphosphate | 0.55 | 0.68 | 0.81 calc. | pH 7.5, 25 °C | Open record: D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB), D-fructose 1,6-bisphosphate |
| D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB) | Escherichia coli | D-glycero-alpha-D-manno-heptose 1,7-bisphosphate | 4.6 | 0.067 | 70 | pH 7.5, 25 °C | Open record: D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB), D-glycero-alpha-D-manno-heptose 1,7-bisphosphate |
| D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB) | Escherichia coli | D-glycero-beta-D-manno-heptose 1,7-bisphosphate | 35.7 | 0.005 | 7 × 10³ | pH 7.5, 25 °C | Open record: D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB), D-glycero-beta-D-manno-heptose 1,7-bisphosphate |
| D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB)variant C107A | Escherichia coli | D-glycero-alpha-D-manno-heptose 1,7-bisphosphate | 0.042 | 0.32 | 0.13 calc. | pH 7.5, 25 °C | Open record: D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB) variant C107A, D-glycero-alpha-D-manno-heptose 1,7-bisphosphate |
| D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB)variant C107A | Escherichia coli | D-glycero-beta-D-manno-heptose 1,7-bisphosphate | 0.63 | 0.083 | 8 | pH 7.5, 25 °C | Open record: D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB) variant C107A, D-glycero-beta-D-manno-heptose 1,7-bisphosphate |
| D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB)variant C109A | Escherichia coli | D-glycero-alpha-D-manno-heptose 1,7-bisphosphate | 0.057 | 0.045 | 1 | pH 7.5, 25 °C | Open record: D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB) variant C109A, D-glycero-alpha-D-manno-heptose 1,7-bisphosphate |
| D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB)variant C109A | Escherichia coli | D-glycero-beta-D-manno-heptose 1,7-bisphosphate | 0.34 | 0.068 | 5 | pH 7.5, 25 °C | Open record: D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB) variant C109A, D-glycero-beta-D-manno-heptose 1,7-bisphosphate |
| D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB)variant C92A | Escherichia coli | D-glycero-alpha-D-manno-heptose 1,7-bisphosphate | 0.0017 | 0.3 | 0.005 | pH 7.5, 25 °C | Open record: D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB) variant C92A, D-glycero-alpha-D-manno-heptose 1,7-bisphosphate |
| D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB)variant C92A | Escherichia coli | D-glycero-beta-D-manno-heptose 1,7-bisphosphate | 0.63 | 0.18 | 30 | pH 7.5, 25 °C | Open record: D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB) variant C92A, D-glycero-beta-D-manno-heptose 1,7-bisphosphate |
| D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB)variant K137A | Escherichia coli | D-glycero-beta-D-manno-heptose 1,7-bisphosphate | 28 | 0.039 | 700 | pH 7.5, 25 °C | Open record: D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB) variant K137A, D-glycero-beta-D-manno-heptose 1,7-bisphosphate |
| D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB)variant R110A | Escherichia coli | D-glycero-beta-D-manno-heptose 1,7-bisphosphate | 6.2 | 0.181 | 30 | pH 7.5, 25 °C | Open record: D-glycero-D-manno-heptose-1,7-bisphosphate phosphatase (GmhB) variant R110A, D-glycero-beta-D-manno-heptose 1,7-bisphosphate |