Values CatLog read from this paper
beta-D-Xylosidase from Selenomonas ruminantium: role of glutamate 186 in catalysis revealed by site-directed mutagenesis, alternate substrates, and active-site inhibitor
PMID 20127424doi:10.1007/s12010-009-8874-7Open in Browse
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CatLog read 14 records (37 values) from this paper: beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA) from Selenomonas ruminantium.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA)variant E186A | Selenomonas ruminantium | 4-nitrophenyl-alpha-L-arabinofuranoside | 4.48 × 10⁻⁶ | 0.177 | 2.53 × 10⁻⁵ | pH 6, 25 °C | Open record: beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA) variant E186A, 4-nitrophenyl-alpha-L-arabinofuranoside |
| beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA)variant E186A | Selenomonas ruminantium | 4-nitrophenyl-alpha-L-arabinofuranoside | 3.61 × 10⁻⁶ | 0.152 | 2.37 × 10⁻⁵ | pH 7, 25 °C | Open record: beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA) variant E186A, 4-nitrophenyl-alpha-L-arabinofuranoside |
| beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA)variant E186A | Selenomonas ruminantium | 4-nitrophenyl-alpha-L-arabinofuranoside | 4.39 × 10⁻⁶ | 0.133 | 3.29 × 10⁻⁵ | pH 8, 25 °C | Open record: beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA) variant E186A, 4-nitrophenyl-alpha-L-arabinofuranoside |
| beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA)variant E186A | Selenomonas ruminantium | 4-nitrophenyl-beta-D-xylopyranosidesubstrate and triethanolamine varied; E186A preparation 7 | 0.00144 | 2.82 | 5.1 × 10⁻⁴ | pH 6, 25 °C | Open record: beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA) variant E186A, 4-nitrophenyl-beta-D-xylopyranoside |
| beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA)variant E186A | Selenomonas ruminantium | 4-nitrophenyl-beta-D-xylopyranosidesubstrate and triethanolamine varied; E186A preparation 8 | 0.00164 | 3.27 | 5.03 × 10⁻⁴ | pH 7, 25 °C | Open record: beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA) variant E186A, 4-nitrophenyl-beta-D-xylopyranoside |
| beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA)variant E186A | Selenomonas ruminantium | 4-nitrophenyl-beta-D-xylopyranoside | 0.0016 | 2.57 | 6.23 × 10⁻⁴ | pH 8, 25 °C | Open record: beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA) variant E186A, 4-nitrophenyl-beta-D-xylopyranoside |
| beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA)variant E186A | Selenomonas ruminantium | 4-nitrophenyl-beta-D-xylopyranosidesubstrate and triethanolamine varied; E186A preparation 6 | 0.00174 | 2.9 | 6.01 × 10⁻⁴ | pH 6, 25 °C | Open record: beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA) variant E186A, 4-nitrophenyl-beta-D-xylopyranoside |
| beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA)variant E186A | Selenomonas ruminantium | 4-nitrophenyl-beta-D-xylopyranosidesubstrate and triethanolamine varied; E186A preparation 7 | 0.00154 | 2.59 | 5.96 × 10⁻⁴ | pH 7, 25 °C | Open record: beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA) variant E186A, 4-nitrophenyl-beta-D-xylopyranoside |
| beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA)variant E186A | Selenomonas ruminantium | 4-nitrophenyl-beta-D-xylopyranosidesubstrate and triethanolamine varied; E186A preparation 5 | 0.00145 | 2.73 | 5.3 × 10⁻⁴ | pH 6, 25 °C | Open record: beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA) variant E186A, 4-nitrophenyl-beta-D-xylopyranoside |
| beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA)variant E186A | Selenomonas ruminantium | 4-nitrophenyl-beta-D-xylopyranosidecontinuous assay at 400 nm (method A); E186A preparation 1 | — | — | 6.35 × 10⁻⁴ | pH 7, 25 °C | Open record: beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA) variant E186A, 4-nitrophenyl-beta-D-xylopyranoside |
| beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA)variant E186A | Selenomonas ruminantium | 4-nitrophenyl-beta-D-xylopyranosidefit to Eq. 2; continuous assay at 400 nm (method A) | 0.00186 | 2.72 | 6.84 × 10⁻⁴ calc. | pH 7, 25 °C | Open record: beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA) variant E186A, 4-nitrophenyl-beta-D-xylopyranoside |
| beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA)variant E186A | Selenomonas ruminantium | 4-nitrophenyl-beta-D-xylopyranosidev_enz2 from fits of the individual Fig. 1c curves to Eq. 3 | 0.00197 | 3.69 | 5.35 × 10⁻⁴ | pH 6, 25 °C | Open record: beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA) variant E186A, 4-nitrophenyl-beta-D-xylopyranoside |
| beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA)variant E186A | Selenomonas ruminantium | 4-nitrophenyl-beta-D-xylopyranosidesecondary plot versus [4NPX] fitted to Eq. 5 | — | 9.03 | — | pH 6, 25 °C | Open record: beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA) variant E186A, 4-nitrophenyl-beta-D-xylopyranoside |
| beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA)variant E186G | Selenomonas ruminantium | 4-nitrophenyl-beta-D-xylopyranoside | 6 × 10⁻⁴ | 2.17 | 2.76 × 10⁻⁴ | pH 8, 25 °C | Open record: beta-D-xylosidase/alpha-L-arabinofuranosidase (SXA) variant E186G, 4-nitrophenyl-beta-D-xylopyranoside |