Values CatLog read from this paper
Electrostatic interactions play a significant role in the affinity of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase for Mn2+
PMID 20211682doi:10.1016/j.biochi.2010.02.032Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 32 records (47 values) from this paper: phosphoenolpyruvate carboxykinase from Saccharomyces cerevisiae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 15 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| phosphoenolpyruvate carboxykinase | Saccharomyces cerevisiae | bicarbonate | — | 17 | — | pH 6.9, 30 °C | Open record: phosphoenolpyruvate carboxykinase, bicarbonate |
| phosphoenolpyruvate carboxykinase | Saccharomyces cerevisiae | MnADP− | — | 0.013 | — | pH 7, 30 °C | Open record: phosphoenolpyruvate carboxykinase, MnADP− |
| phosphoenolpyruvate carboxykinase | Saccharomyces cerevisiae | oxaloacetateno ADP | 0.28 | 0.13 | 2.2 calc. | pH 7, 30 °C | Open record: phosphoenolpyruvate carboxykinase, oxaloacetate |
| phosphoenolpyruvate carboxykinase | Saccharomyces cerevisiae | oxaloacetatein the presence of 400 μM ADP (footnote b) | 0.26 | 0.063 | 4.1 calc. | pH 7, 30 °C | Open record: phosphoenolpyruvate carboxykinase, oxaloacetate |
| phosphoenolpyruvate carboxykinase | Saccharomyces cerevisiae | phosphoenolpyruvate | 0.46 | — | — | pH 7, 30 °C | Open record: phosphoenolpyruvate carboxykinase, phosphoenolpyruvate |
| phosphoenolpyruvate carboxykinasevariant E272Q | Saccharomyces cerevisiae | bicarbonate | — | 9 | — | pH 6.9, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant E272Q, bicarbonate |
| phosphoenolpyruvate carboxykinasevariant E272Q | Saccharomyces cerevisiae | MnADP− | — | 0.027 | — | pH 6.9, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant E272Q, MnADP− |
| phosphoenolpyruvate carboxykinasevariant E272Q | Saccharomyces cerevisiae | MnADP− | — | 0.015 | — | pH 7, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant E272Q, MnADP− |
| phosphoenolpyruvate carboxykinasevariant E272Q | Saccharomyces cerevisiae | oxaloacetate | 0.08 | 0.238 | 0.34 calc. | pH 7, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant E272Q, oxaloacetate |
| phosphoenolpyruvate carboxykinasevariant E272Q | Saccharomyces cerevisiae | phosphoenolpyruvate | 19 | 0.185 | 100 calc. | pH 6.9, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant E272Q, phosphoenolpyruvate |
| phosphoenolpyruvate carboxykinasevariant E272Q | Saccharomyces cerevisiae | phosphoenolpyruvate | 1.7 | 0.058 | 29 calc. | pH 7, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant E272Q, phosphoenolpyruvate |
| phosphoenolpyruvate carboxykinasevariant E284Q | Saccharomyces cerevisiae | bicarbonate | — | 14 | — | pH 6.9, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant E284Q, bicarbonate |
| phosphoenolpyruvate carboxykinasevariant E284Q | Saccharomyces cerevisiae | MnADP− | — | 0.021 | — | pH 6.9, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant E284Q, MnADP− |
| phosphoenolpyruvate carboxykinasevariant E284Q | Saccharomyces cerevisiae | MnADP− | — | 0.191 | — | pH 7, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant E284Q, MnADP− |
| phosphoenolpyruvate carboxykinasevariant E284Q | Saccharomyces cerevisiae | oxaloacetate | 0.8 | — | — | pH 7, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant E284Q, oxaloacetate |
| phosphoenolpyruvate carboxykinasevariant E284Q | Saccharomyces cerevisiae | phosphoenolpyruvate | 0.07 | 0.17 | 0.41 calc. | pH 6.9, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant E284Q, phosphoenolpyruvate |
| phosphoenolpyruvate carboxykinasevariant E284Q | Saccharomyces cerevisiae | phosphoenolpyruvate | 0.2 | 0.09 | 2.2 calc. | pH 7, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant E284Q, phosphoenolpyruvate |
| phosphoenolpyruvate carboxykinasevariant K212M | Saccharomyces cerevisiae | bicarbonate | — | 23 | — | pH 6.9, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant K212M, bicarbonate |
| phosphoenolpyruvate carboxykinasevariant K212M | Saccharomyces cerevisiae | MnADP− | — | 0.212 | — | pH 6.9, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant K212M, MnADP− |
| phosphoenolpyruvate carboxykinasevariant K212M | Saccharomyces cerevisiae | MnADP− | — | 0.022 | — | pH 7, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant K212M, MnADP− |
| phosphoenolpyruvate carboxykinasevariant K212M | Saccharomyces cerevisiae | oxaloacetate | 0.85 | 0.068 | 12 calc. | pH 7, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant K212M, oxaloacetate |
| phosphoenolpyruvate carboxykinasevariant K212M | Saccharomyces cerevisiae | phosphoenolpyruvate | 0.13 | 0.216 | 0.60 calc. | pH 6.9, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant K212M, phosphoenolpyruvate |
| phosphoenolpyruvate carboxykinasevariant K212M | Saccharomyces cerevisiae | phosphoenolpyruvate | 1.8 | 0.044 | 41 calc. | pH 7, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant K212M, phosphoenolpyruvate |
| phosphoenolpyruvate carboxykinasevariant K212R | Saccharomyces cerevisiae | MnADP− | — | 0.03 | — | pH 6.9, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant K212R, MnADP− |
| phosphoenolpyruvate carboxykinasevariant K212R | Saccharomyces cerevisiae | oxaloacetate | 0.11 | 0.2 | 0.55 calc. | pH 7, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant K212R, oxaloacetate |
| phosphoenolpyruvate carboxykinasevariant K212R | Saccharomyces cerevisiae | phosphoenolpyruvate | 0.014 | 0.651 | 0.022 calc. | pH 6.9, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant K212R, phosphoenolpyruvate |
| phosphoenolpyruvate carboxykinasevariant K213Q | Saccharomyces cerevisiae | bicarbonate | — | 11 | — | pH 6.9, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant K213Q, bicarbonate |
| phosphoenolpyruvate carboxykinasevariant K213Q | Saccharomyces cerevisiae | MnADP− | — | 0.135 | — | pH 6.9, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant K213Q, MnADP− |
| phosphoenolpyruvate carboxykinasevariant K213Q | Saccharomyces cerevisiae | MnADP− | — | 0.116 | — | pH 7, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant K213Q, MnADP− |
| phosphoenolpyruvate carboxykinasevariant K213Q | Saccharomyces cerevisiae | oxaloacetate | 0.58 | 0.024 | 24 calc. | pH 7, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant K213Q, oxaloacetate |
| phosphoenolpyruvate carboxykinasevariant K213Q | Saccharomyces cerevisiae | phosphoenolpyruvate | 5.1 | 0.32 | 16 calc. | pH 6.9, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant K213Q, phosphoenolpyruvate |
| phosphoenolpyruvate carboxykinasevariant K213Q | Saccharomyces cerevisiae | phosphoenolpyruvate | 4.3 | 0.178 | 24 calc. | pH 7, 30 °C | Open record: phosphoenolpyruvate carboxykinase variant K213Q, phosphoenolpyruvate |