Values CatLog read from this paper
Glutamates 78 and 122 in the active site of saccharopine dehydrogenase contribute to reactant binding and modulate the basicity of the acid-base catalysts
PMID 20427272doi:10.1074/jbc.m110.119826Open in Browse
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CatLog read 42 records (74 values) from this paper: saccharopine dehydrogenase from Saccharomyces cerevisiae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| saccharopine dehydrogenasevariant E122A | Saccharomyces cerevisiae | 2-oxoglutarate | — | 0.556 | 35 | pH 7.2, 25 °C | Open record: saccharopine dehydrogenase variant E122A, 2-oxoglutarate |
| saccharopine dehydrogenasevariant E122A | Saccharomyces cerevisiae | 2-oxoglutarate | — | — | 19.6 | 25 °C | Open record: saccharopine dehydrogenase variant E122A, 2-oxoglutarate |
| saccharopine dehydrogenasevariant E122A | Saccharomyces cerevisiae | beta-NADH | — | 0.062 | 310 | pH 7.2, 25 °C | Open record: saccharopine dehydrogenase variant E122A, beta-NADH |
| saccharopine dehydrogenasevariant E122A | Saccharomyces cerevisiae | L-lysine | 19.4 | 36.5 | 0.53 | pH 7.2, 25 °C | Open record: saccharopine dehydrogenase variant E122A, L-lysine |
| saccharopine dehydrogenasevariant E122A | Saccharomyces cerevisiae | L-lysine | 14.12 | — | — | 25 °C | Open record: saccharopine dehydrogenase variant E122A, L-lysine |
| saccharopine dehydrogenasevariant E122A | Saccharomyces cerevisiae | L-lysine | — | — | 0.028 | 25 °C | Open record: saccharopine dehydrogenase variant E122A, L-lysine |
| saccharopine dehydrogenasevariant E122A | Saccharomyces cerevisiae | L-lysine | — | — | 0.49 | 25 °C | Open record: saccharopine dehydrogenase variant E122A, L-lysine |
| saccharopine dehydrogenasevariant E122Q | Saccharomyces cerevisiae | 2-oxoglutarate | — | 0.3 | 15 | pH 7.2, 25 °C | Open record: saccharopine dehydrogenase variant E122Q, 2-oxoglutarate |
| saccharopine dehydrogenasevariant E122Q | Saccharomyces cerevisiae | 2-oxoglutarate | — | — | 19 | 25 °C | Open record: saccharopine dehydrogenase variant E122Q, 2-oxoglutarate |
| saccharopine dehydrogenasevariant E122Q | Saccharomyces cerevisiae | beta-NAD+ | — | 3.3 | 0.231 | pH 7.2, 25 °C | Open record: saccharopine dehydrogenase variant E122Q, beta-NAD+ |
| saccharopine dehydrogenasevariant E122Q | Saccharomyces cerevisiae | beta-NADH | — | 0.025 | 190 | pH 7.2, 25 °C | Open record: saccharopine dehydrogenase variant E122Q, beta-NADH |
| saccharopine dehydrogenasevariant E122Q | Saccharomyces cerevisiae | L-lysine | 4.3 | 11 | 0.41 | pH 7.2, 25 °C | Open record: saccharopine dehydrogenase variant E122Q, L-lysine |
| saccharopine dehydrogenasevariant E122Q | Saccharomyces cerevisiae | L-lysine | 5.09 | — | 0.505 | 25 °C | Open record: saccharopine dehydrogenase variant E122Q, L-lysine |
| saccharopine dehydrogenasevariant E122Q | Saccharomyces cerevisiae | N6-(glutaryl-2)-L-lysine | 0.76 | 14 | 0.055 | pH 7.2, 25 °C | Open record: saccharopine dehydrogenase variant E122Q, N6-(glutaryl-2)-L-lysine |
| saccharopine dehydrogenasevariant E78A | Saccharomyces cerevisiae | 2-oxoglutarate | — | 0.18 | 490 | pH 7.2, 25 °C | Open record: saccharopine dehydrogenase variant E78A, 2-oxoglutarate |
| saccharopine dehydrogenasevariant E78A | Saccharomyces cerevisiae | 2-oxoglutarate | — | — | 420 | 25 °C | Open record: saccharopine dehydrogenase variant E78A, 2-oxoglutarate |
| saccharopine dehydrogenasevariant E78A | Saccharomyces cerevisiae | beta-NADH | — | 0.036 | 2.5 × 10³ | pH 7.2, 25 °C | Open record: saccharopine dehydrogenase variant E78A, beta-NADH |
| saccharopine dehydrogenasevariant E78A | Saccharomyces cerevisiae | L-lysine | 88.8 | 0.62 | 143 | pH 7.2, 25 °C | Open record: saccharopine dehydrogenase variant E78A, L-lysine |
| saccharopine dehydrogenasevariant E78A | Saccharomyces cerevisiae | L-lysine | 150 | — | — | 25 °C | Open record: saccharopine dehydrogenase variant E78A, L-lysine |
| saccharopine dehydrogenasevariant E78A | Saccharomyces cerevisiae | L-lysine | 11.6 | — | — | 25 °C | Open record: saccharopine dehydrogenase variant E78A, L-lysine |
| saccharopine dehydrogenasevariant E78A | Saccharomyces cerevisiae | L-lysine | — | — | 180 | 25 °C | Open record: saccharopine dehydrogenase variant E78A, L-lysine |
| saccharopine dehydrogenasevariant E78A/E122A | Saccharomyces cerevisiae | 2-oxoglutarate | — | 4.85 | 5.11 | pH 7.2, 25 °C | Open record: saccharopine dehydrogenase variant E78A/E122A, 2-oxoglutarate |
| saccharopine dehydrogenasevariant E78A/E122A | Saccharomyces cerevisiae | beta-NADH | — | 0.113 | 220 | pH 7.2, 25 °C | Open record: saccharopine dehydrogenase variant E78A/E122A, beta-NADH |
| saccharopine dehydrogenasevariant E78A/E122A | Saccharomyces cerevisiae | L-lysine | 24.8 | 190.7 | 0.13 | pH 7.2, 25 °C | Open record: saccharopine dehydrogenase variant E78A/E122A, L-lysine |
| saccharopine dehydrogenasevariant E78A/E122A | Saccharomyces cerevisiae | L-lysine | 22.4 | — | — | 25 °C | Open record: saccharopine dehydrogenase variant E78A/E122A, L-lysine |
| saccharopine dehydrogenasevariant E78A/E122A | Saccharomyces cerevisiae | L-lysine | — | — | 0.027 | 25 °C | Open record: saccharopine dehydrogenase variant E78A/E122A, L-lysine |
| saccharopine dehydrogenasevariant E78A/E122A | Saccharomyces cerevisiae | L-lysine | — | — | 0.219 | 25 °C | Open record: saccharopine dehydrogenase variant E78A/E122A, L-lysine |
| saccharopine dehydrogenasevariant E78Q | Saccharomyces cerevisiae | 2-oxoglutarate | — | 0.23 | 49 | pH 7.2, 25 °C | Open record: saccharopine dehydrogenase variant E78Q, 2-oxoglutarate |
| saccharopine dehydrogenasevariant E78Q | Saccharomyces cerevisiae | 2-oxoglutarate | — | — | 99.2 | 25 °C | Open record: saccharopine dehydrogenase variant E78Q, 2-oxoglutarate |
| saccharopine dehydrogenasevariant E78Q | Saccharomyces cerevisiae | beta-NAD+ | — | 1.1 | 3.62 | pH 7.2, 25 °C | Open record: saccharopine dehydrogenase variant E78Q, beta-NAD+ |
| saccharopine dehydrogenasevariant E78Q | Saccharomyces cerevisiae | beta-NADH | — | 0.014 | 800 | pH 7.2, 25 °C | Open record: saccharopine dehydrogenase variant E78Q, beta-NADH |
| saccharopine dehydrogenasevariant E78Q | Saccharomyces cerevisiae | L-lysine | 11.2 | 4 | 2.8 | pH 7.2, 25 °C | Open record: saccharopine dehydrogenase variant E78Q, L-lysine |
| saccharopine dehydrogenasevariant E78Q | Saccharomyces cerevisiae | L-lysine | 2 | — | 5.2 | 25 °C | Open record: saccharopine dehydrogenase variant E78Q, L-lysine |
| saccharopine dehydrogenasevariant E78Q | Saccharomyces cerevisiae | N6-(glutaryl-2)-L-lysine | 3.95 | 2 | 2.1 | pH 7.2, 25 °C | Open record: saccharopine dehydrogenase variant E78Q, N6-(glutaryl-2)-L-lysine |
| saccharopine dehydrogenasevariant E78Q/E122Q | Saccharomyces cerevisiae | 2-oxoglutarate | — | 2 | 13 | pH 7.2, 25 °C | Open record: saccharopine dehydrogenase variant E78Q/E122Q, 2-oxoglutarate |
| saccharopine dehydrogenasevariant E78Q/E122Q | Saccharomyces cerevisiae | beta-NADH | — | 0.12 | 200 | pH 7.2, 25 °C | Open record: saccharopine dehydrogenase variant E78Q/E122Q, beta-NADH |
| saccharopine dehydrogenasevariant E78Q/E122Q | Saccharomyces cerevisiae | L-lysine | 24.7 | 27.1 | 0.91 | pH 7.2, 25 °C | Open record: saccharopine dehydrogenase variant E78Q/E122Q, L-lysine |
| saccharopine dehydrogenasevariant E78Q/E122Q | Saccharomyces cerevisiae | L-lysine | 20.9 | — | — | 25 °C | Open record: saccharopine dehydrogenase variant E78Q/E122Q, L-lysine |
| saccharopine dehydrogenasevariant E78Q/E122Q | Saccharomyces cerevisiae | L-lysine | 5.24 | — | — | 25 °C | Open record: saccharopine dehydrogenase variant E78Q/E122Q, L-lysine |
| saccharopine dehydrogenasevariant E78Q/E122Q | Saccharomyces cerevisiae | L-lysine | — | — | 0.0756 | 25 °C | Open record: saccharopine dehydrogenase variant E78Q/E122Q, L-lysine |
| saccharopine dehydrogenasevariant E78Q/E122Q | Saccharomyces cerevisiae | L-lysine | — | — | 0.0292 | 25 °C | Open record: saccharopine dehydrogenase variant E78Q/E122Q, L-lysine |
| saccharopine dehydrogenasevariant E78Q/E122Q | Saccharomyces cerevisiae | L-lysine | — | — | 0.00206 | 25 °C | Open record: saccharopine dehydrogenase variant E78Q/E122Q, L-lysine |