Values CatLog read from this paper
Kinetic and structural characterization of a heterohexamer 4-oxalocrotonate tautomerase from Chloroflexus aurantiacus J-10-fl: implications for functional and structural diversity in the tautomerase…
PMID 20465238doi:10.1021/bi100502zOpen in Browse
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CatLog read 14 records (42 values) from this paper: heterohexamer 4-oxalocrotonate tautomerase (hh4-OT), 4-oxalocrotonate tautomerase (4-OT, homohexamer) from Chloroflexus aurantiacus, Pseudomonas putida.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 4-oxalocrotonate tautomerase (4-OT, homohexamer) | Pseudomonas putida | 2-hydroxy-2,4-hexadienedioate | 4 × 10³ | 0.062 | 6.5 × 10⁴ | pH 7.3, 24 °C | Open record: 4-oxalocrotonate tautomerase (4-OT, homohexamer), 2-hydroxy-2,4-hexadienedioate |
| 4-oxalocrotonate tautomerase (4-OT, homohexamer) | Pseudomonas putida | phenylenolpyruvate | 73 | 0.199 | 370 | pH 7.3, 24 °C | Open record: 4-oxalocrotonate tautomerase (4-OT, homohexamer), phenylenolpyruvate |
| heterohexamer 4-oxalocrotonate tautomerase (hh4-OT) | Chloroflexus aurantiacus | 2-hydroxy-2,4-hexadienedioate | 3 × 10³ | 0.07 | 4.3 × 10⁴ | pH 7.3, 24 °C | Open record: heterohexamer 4-oxalocrotonate tautomerase (hh4-OT), 2-hydroxy-2,4-hexadienedioate |
| heterohexamer 4-oxalocrotonate tautomerase (hh4-OT) | Chloroflexus aurantiacus | phenylenolpyruvate | 13 | 0.121 | 110 | pH 7.3, 24 °C | Open record: heterohexamer 4-oxalocrotonate tautomerase (hh4-OT), phenylenolpyruvate |
| heterohexamer 4-oxalocrotonate tautomerase (hh4-OT)variant αR12A | Chloroflexus aurantiacus | 2-hydroxy-2,4-hexadienedioate | 43 | 1.033 | 42 | pH 7.3, 24 °C | Open record: heterohexamer 4-oxalocrotonate tautomerase (hh4-OT) variant αR12A, 2-hydroxy-2,4-hexadienedioate |
| heterohexamer 4-oxalocrotonate tautomerase (hh4-OT)variant αR12A | Chloroflexus aurantiacus | phenylenolpyruvate | 0.6 | 0.143 | 4.2 | pH 7.3, 24 °C | Open record: heterohexamer 4-oxalocrotonate tautomerase (hh4-OT) variant αR12A, phenylenolpyruvate |
| heterohexamer 4-oxalocrotonate tautomerase (hh4-OT)variant αR40A | Chloroflexus aurantiacus | 2-hydroxy-2,4-hexadienedioate | 65 | 0.345 | 190 | pH 7.3, 24 °C | Open record: heterohexamer 4-oxalocrotonate tautomerase (hh4-OT) variant αR40A, 2-hydroxy-2,4-hexadienedioate |
| heterohexamer 4-oxalocrotonate tautomerase (hh4-OT)variant αR40A | Chloroflexus aurantiacus | phenylenolpyruvate | 44 | 0.152 | 290 | pH 7.3, 24 °C | Open record: heterohexamer 4-oxalocrotonate tautomerase (hh4-OT) variant αR40A, phenylenolpyruvate |
| heterohexamer 4-oxalocrotonate tautomerase (hh4-OT)variant βP1A | Chloroflexus aurantiacus | 2-hydroxy-2,4-hexadienedioate | 36 | 0.017 | 2.1 × 10³ | pH 7.3, 24 °C | Open record: heterohexamer 4-oxalocrotonate tautomerase (hh4-OT) variant βP1A, 2-hydroxy-2,4-hexadienedioate |
| heterohexamer 4-oxalocrotonate tautomerase (hh4-OT)variant βP1A | Chloroflexus aurantiacus | phenylenolpyruvate | 0.2 | 0.063 | 3.2 | pH 7.3, 24 °C | Open record: heterohexamer 4-oxalocrotonate tautomerase (hh4-OT) variant βP1A, phenylenolpyruvate |
| heterohexamer 4-oxalocrotonate tautomerase (hh4-OT)variant βR11A | Chloroflexus aurantiacus | 2-hydroxy-2,4-hexadienedioate | 3.5 × 10³ | 0.069 | 5.1 × 10⁴ | pH 7.3, 24 °C | Open record: heterohexamer 4-oxalocrotonate tautomerase (hh4-OT) variant βR11A, 2-hydroxy-2,4-hexadienedioate |
| heterohexamer 4-oxalocrotonate tautomerase (hh4-OT)variant βR11A | Chloroflexus aurantiacus | phenylenolpyruvate | 17 | 0.198 | 86 | pH 7.3, 24 °C | Open record: heterohexamer 4-oxalocrotonate tautomerase (hh4-OT) variant βR11A, phenylenolpyruvate |
| heterohexamer 4-oxalocrotonate tautomerase (hh4-OT)variant βR39A | Chloroflexus aurantiacus | 2-hydroxy-2,4-hexadienedioate | 733 | 0.135 | 5.4 × 10³ | pH 7.3, 24 °C | Open record: heterohexamer 4-oxalocrotonate tautomerase (hh4-OT) variant βR39A, 2-hydroxy-2,4-hexadienedioate |
| heterohexamer 4-oxalocrotonate tautomerase (hh4-OT)variant βR39A | Chloroflexus aurantiacus | phenylenolpyruvate | 11 | 0.159 | 70 | pH 7.3, 24 °C | Open record: heterohexamer 4-oxalocrotonate tautomerase (hh4-OT) variant βR39A, phenylenolpyruvate |