Values CatLog read from this paper
Increasing the conformational entropy of the Omega-loop lid domain in phosphoenolpyruvate carboxykinase impairs catalysis and decreases catalytic fidelity
PMID 20476774doi:10.1021/bi100399eOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 14 records (35 values) from this paper: phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK) from Rattus norvegicus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK) | Rattus norvegicus | carbon dioxide | — | 1.194 | 16 | pH 7.5, 25 °C | Open record: phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK), carbon dioxide |
| phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK) | Rattus norvegicus | guanosine-5'-diphosphate | — | 0.039 | 510 | pH 7.5, 25 °C | Open record: phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK), guanosine-5'-diphosphate |
| phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK) | Rattus norvegicus | guanosine-5'-O-[gamma-thio]triphosphate | 13 | 0.039 | 330 calc. | pH 7.5, 25 °C | Open record: phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK), guanosine-5'-O-[gamma-thio]triphosphate |
| phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK) | Rattus norvegicus | guanosine-5'-triphosphate | — | 0.068 | 790 | pH 7.5, 25 °C | Open record: phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK), guanosine-5'-triphosphate |
| phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK) | Rattus norvegicus | oxaloacetic acidAssay A; OAA + GTP -> PEP + GDP + CO2 (OAA varied) | 54 | 0.052 | 1 × 10³ | pH 7.5, 25 °C | Open record: phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK), oxaloacetic acid |
| phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK) | Rattus norvegicus | oxaloacetic acidAssay D; 500 µM GDP; coupled assay: 30 U LDH | 2.3 | 0.117 | 20 | pH 7.5, 25 °C | Open record: phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK), oxaloacetic acid |
| phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK) | Rattus norvegicus | phosphoenolpyruvate | 19 | 0.294 | 66 | pH 7.5, 25 °C | Open record: phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK), phosphoenolpyruvate |
| phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK)variant A467G | Rattus norvegicus | carbon dioxide | — | 0.814 | 1.2 | pH 7.5, 25 °C | Open record: phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK) variant A467G, carbon dioxide |
| phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK)variant A467G | Rattus norvegicus | guanosine-5'-diphosphate | — | 0.07 | 14 | pH 7.5, 25 °C | Open record: phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK) variant A467G, guanosine-5'-diphosphate |
| phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK)variant A467G | Rattus norvegicus | guanosine-5'-triphosphate | — | 0.047 | 270 | pH 7.5, 25 °C | Open record: phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK) variant A467G, guanosine-5'-triphosphate |
| phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK)variant A467G | Rattus norvegicus | oxaloacetic acidAssay A; OAA + GTP -> PEP + GDP + CO2 (OAA varied) | 14 | 0.749 | 19 | pH 7.5, 25 °C | Open record: phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK) variant A467G, oxaloacetic acid |
| phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK)variant A467G | Rattus norvegicus | oxaloacetic acidAssay C; 500 µM GTP; coupled assay: 30 U LDH | 2.6 | 0.512 | 5 | pH 7.5, 25 °C | Open record: phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK) variant A467G, oxaloacetic acid |
| phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK)variant A467G | Rattus norvegicus | oxaloacetic acidAssay D; 500 µM GDP; coupled assay: 30 U LDH | 1.3 | 0.97 | 1 | pH 7.5, 25 °C | Open record: phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK) variant A467G, oxaloacetic acid |
| phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK)variant A467G | Rattus norvegicus | phosphoenolpyruvate | 1 | 0.063 | 15 | pH 7.5, 25 °C | Open record: phosphoenolpyruvate carboxykinase (cytosolic, GTP-dependent; cPEPCK) variant A467G, phosphoenolpyruvate |