Values CatLog read from this paper
A novel nucleotide kinase encoded by gene 1.7 of bacteriophage T7
PMID 20497505doi:10.1111/j.1365-2958.2010.07221.xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 19 records (38 values) from this paper: gp1.7 nucleotide kinase (gene 1.7 product), thymidylate kinase (TMK) from bacteriophage T7, Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 19 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| gp1.7 nucleotide kinase (gene 1.7 product) | bacteriophage T7 | 2'-deoxyguanosine 5'-monophosphatecosubstrate GTP | 1.2 | 0.02 | 60 calc. | pH 7.5, 37 °C | Open record: gp1.7 nucleotide kinase (gene 1.7 product), 2'-deoxyguanosine 5'-monophosphate |
| gp1.7 nucleotide kinase (gene 1.7 product) | bacteriophage T7 | 2'-deoxyguanosine 5'-monophosphatecosubstrate dGTP | 5.6 | 0.035 | 160 calc. | pH 7.5, 37 °C | Open record: gp1.7 nucleotide kinase (gene 1.7 product), 2'-deoxyguanosine 5'-monophosphate |
| gp1.7 nucleotide kinase (gene 1.7 product) | bacteriophage T7 | 2'-deoxyguanosine 5'-monophosphatecosubstrate dTTP | 5 | 0.039 | 130 calc. | pH 7.5, 37 °C | Open record: gp1.7 nucleotide kinase (gene 1.7 product), 2'-deoxyguanosine 5'-monophosphate |
| gp1.7 nucleotide kinase (gene 1.7 product) | bacteriophage T7 | thymidine 5'-monophosphatecosubstrate GTP | 1.7 | 0.173 | 9.8 calc. | pH 7.5, 37 °C | Open record: gp1.7 nucleotide kinase (gene 1.7 product), thymidine 5'-monophosphate |
| gp1.7 nucleotide kinase (gene 1.7 product) | bacteriophage T7 | thymidine 5'-monophosphatecosubstrate dGTP | 4.3 | 0.07 | 61 calc. | pH 7.5, 37 °C | Open record: gp1.7 nucleotide kinase (gene 1.7 product), thymidine 5'-monophosphate |
| gp1.7 nucleotide kinase (gene 1.7 product) | bacteriophage T7 | thymidine 5'-monophosphatecosubstrate dTTP | 3.5 | 0.077 | 45 calc. | pH 7.5, 37 °C | Open record: gp1.7 nucleotide kinase (gene 1.7 product), thymidine 5'-monophosphate |
| gp1.7 nucleotide kinase (gene 1.7 product)variant Start2 Met→Leu (internal initiation codon ATG→CTG) | bacteriophage T7 | 2'-deoxyguanosine 5'-monophosphatecosubstrate GTP | 1.3 | 0.023 | 57 calc. | pH 7.5, 37 °C | Open record: gp1.7 nucleotide kinase (gene 1.7 product) variant Start2 Met→Leu (internal initiation codon ATG→CTG), 2'-deoxyguanosine 5'-monophosphate |
| gp1.7 nucleotide kinase (gene 1.7 product)variant Start2 Met→Leu (internal initiation codon ATG→CTG) | bacteriophage T7 | 2'-deoxyguanosine 5'-monophosphatecosubstrate dGTP | 5.7 | 0.037 | 150 calc. | pH 7.5, 37 °C | Open record: gp1.7 nucleotide kinase (gene 1.7 product) variant Start2 Met→Leu (internal initiation codon ATG→CTG), 2'-deoxyguanosine 5'-monophosphate |
| gp1.7 nucleotide kinase (gene 1.7 product)variant Start2 Met→Leu (internal initiation codon ATG→CTG) | bacteriophage T7 | 2'-deoxyguanosine 5'-monophosphatecosubstrate dTTP | 5.1 | 0.04 | 130 calc. | pH 7.5, 37 °C | Open record: gp1.7 nucleotide kinase (gene 1.7 product) variant Start2 Met→Leu (internal initiation codon ATG→CTG), 2'-deoxyguanosine 5'-monophosphate |
| gp1.7 nucleotide kinase (gene 1.7 product)variant Start2 Met→Leu (internal initiation codon ATG→CTG) | bacteriophage T7 | thymidine 5'-monophosphatecosubstrate GTP | 1.5 | 0.168 | 8.9 calc. | pH 7.5, 37 °C | Open record: gp1.7 nucleotide kinase (gene 1.7 product) variant Start2 Met→Leu (internal initiation codon ATG→CTG), thymidine 5'-monophosphate |
| gp1.7 nucleotide kinase (gene 1.7 product)variant Start2 Met→Leu (internal initiation codon ATG→CTG) | bacteriophage T7 | thymidine 5'-monophosphatecosubstrate dGTP | 4.5 | 0.073 | 62 calc. | pH 7.5, 37 °C | Open record: gp1.7 nucleotide kinase (gene 1.7 product) variant Start2 Met→Leu (internal initiation codon ATG→CTG), thymidine 5'-monophosphate |
| gp1.7 nucleotide kinase (gene 1.7 product)variant Start2 Met→Leu (internal initiation codon ATG→CTG) | bacteriophage T7 | thymidine 5'-monophosphatecosubstrate dTTP | 3.3 | 0.073 | 45 calc. | pH 7.5, 37 °C | Open record: gp1.7 nucleotide kinase (gene 1.7 product) variant Start2 Met→Leu (internal initiation codon ATG→CTG), thymidine 5'-monophosphate |
| gp1.7 nucleotide kinase (gene 1.7 product)Δ41N (18 kDa form) | bacteriophage T7 | 2'-deoxyguanosine 5'-monophosphatecosubstrate GTP | 1.2 | 0.02 | 60 calc. | pH 7.5, 37 °C | Open record: gp1.7 nucleotide kinase (gene 1.7 product) Δ41N (18 kDa form), 2'-deoxyguanosine 5'-monophosphate |
| gp1.7 nucleotide kinase (gene 1.7 product)Δ41N (18 kDa form) | bacteriophage T7 | 2'-deoxyguanosine 5'-monophosphatecosubstrate dGTP | 5.6 | 0.034 | 160 calc. | pH 7.5, 37 °C | Open record: gp1.7 nucleotide kinase (gene 1.7 product) Δ41N (18 kDa form), 2'-deoxyguanosine 5'-monophosphate |
| gp1.7 nucleotide kinase (gene 1.7 product)Δ41N (18 kDa form) | bacteriophage T7 | 2'-deoxyguanosine 5'-monophosphatecosubstrate dTTP | 4.9 | 0.037 | 130 calc. | pH 7.5, 37 °C | Open record: gp1.7 nucleotide kinase (gene 1.7 product) Δ41N (18 kDa form), 2'-deoxyguanosine 5'-monophosphate |
| gp1.7 nucleotide kinase (gene 1.7 product)Δ41N (18 kDa form) | bacteriophage T7 | thymidine 5'-monophosphatecosubstrate GTP | 1.7 | 0.171 | 9.9 calc. | pH 7.5, 37 °C | Open record: gp1.7 nucleotide kinase (gene 1.7 product) Δ41N (18 kDa form), thymidine 5'-monophosphate |
| gp1.7 nucleotide kinase (gene 1.7 product)Δ41N (18 kDa form) | bacteriophage T7 | thymidine 5'-monophosphatecosubstrate dGTP | 4.1 | 0.068 | 60 calc. | pH 7.5, 37 °C | Open record: gp1.7 nucleotide kinase (gene 1.7 product) Δ41N (18 kDa form), thymidine 5'-monophosphate |
| gp1.7 nucleotide kinase (gene 1.7 product)Δ41N (18 kDa form) | bacteriophage T7 | thymidine 5'-monophosphatecosubstrate dTTP | 3.6 | 0.074 | 49 calc. | pH 7.5, 37 °C | Open record: gp1.7 nucleotide kinase (gene 1.7 product) Δ41N (18 kDa form), thymidine 5'-monophosphate |
| thymidylate kinase (TMK) | Escherichia coli | thymidine 5'-monophosphate | 5.7 | 0.027 | 210 calc. | pH 7.5, 37 °C | Open record: thymidylate kinase (TMK), thymidine 5'-monophosphate |