CatLogenzyme kinetics catalog

Values CatLog read from this paper

Arginines 65 and 310 in putidaredoxin reductase are critical for interaction with putidaredoxin

PMID 20524621doi:10.1021/bi100626fOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 32 records (48 values) from this paper: putidaredoxin reductase (Pdr) from Pseudomonas putida.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 16 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.18.1.5

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
putidaredoxin reductase (Pdr)Pseudomonas putida2,6-dichlorophenolindophenol3850.0974.0 × 10³ calc.pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)Pseudomonas putidaNADHDCIP reductase activity followed at 600; cosubstrate DCIP—0.027—pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)Pseudomonas putidaNADHK3Fe(CN)6 reductase activity followed at 420—0.038—pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)Pseudomonas putidapotassium ferricyanide9820.042.5 × 10⁴ calc.pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)variant K339APseudomonas putida2,6-dichlorophenolindophenol288.30.1252.31 × 10³ calc.pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)variant K339APseudomonas putidaNADHDCIP reductase activity followed at 600; cosubstrate DCIP—0.022—pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)variant K339APseudomonas putidaNADHK3Fe(CN)6 reductase activity followed at 420—0.025—pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)variant K339APseudomonas putidapotassium ferricyanide543.30.0451.2 × 10⁴ calc.pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)variant K387APseudomonas putida2,6-dichlorophenolindophenol408.30.0834.9 × 10³ calc.pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)variant K387APseudomonas putidaNADHDCIP reductase activity followed at 600; cosubstrate DCIP—0.042—pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)variant K387APseudomonas putidaNADHK3Fe(CN)6 reductase activity followed at 420—0.036—pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)variant K387APseudomonas putidapotassium ferricyanide771.70.0441.8 × 10⁴ calc.pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)variant K409APseudomonas putida2,6-dichlorophenolindophenol3770.0914.1 × 10³ calc.pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)variant K409APseudomonas putidaNADHDCIP reductase activity followed at 600; cosubstrate DCIP—0.031—pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)variant K409APseudomonas putidaNADHK3Fe(CN)6 reductase activity followed at 420—0.029—pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)variant K409APseudomonas putidapotassium ferricyanide7550.0362.1 × 10⁴ calc.pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)variant N384APseudomonas putida2,6-dichlorophenolindophenol3580.0834.3 × 10³ calc.pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)variant N384APseudomonas putidaNADHDCIP reductase activity followed at 600; cosubstrate DCIP—0.05—pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)variant N384APseudomonas putidaNADHK3Fe(CN)6 reductase activity followed at 420—0.05—pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)variant N384APseudomonas putidapotassium ferricyanide8980.0432.1 × 10⁴ calc.pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)variant R310APseudomonas putida2,6-dichlorophenolindophenol6170.1254.93 × 10³ calc.pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)variant R310APseudomonas putidaNADHDCIP reductase activity followed at 600; cosubstrate DCIP—0.018—pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)variant R310APseudomonas putidaNADHK3Fe(CN)6 reductase activity followed at 420—0.036—pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)variant R310APseudomonas putidapotassium ferricyanide14850.1431.04 × 10⁴ calc.pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)variant R310EPseudomonas putida2,6-dichlorophenolindophenol2830.21.4 × 10³ calc.pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)variant R310EPseudomonas putidaNADHDCIP reductase activity followed at 600; cosubstrate DCIP—0.037—pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)variant R310EPseudomonas putidaNADHK3Fe(CN)6 reductase activity followed at 420—0.037—pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)variant R310EPseudomonas putidapotassium ferricyanide7900.042.0 × 10⁴ calc.pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)variant R65APseudomonas putida2,6-dichlorophenolindophenol2030.0297.0 × 10³ calc.pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)variant R65APseudomonas putidaNADHDCIP reductase activity followed at 600; cosubstrate DCIP—0.017—pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)variant R65APseudomonas putidaNADHK3Fe(CN)6 reductase activity followed at 420—0.034—pH 7.5, 25 °COpen
putidaredoxin reductase (Pdr)variant R65APseudomonas putidapotassium ferricyanide5600.051.1 × 10⁴ calc.pH 7.5, 25 °COpen