Values CatLog read from this paper
On the catalytic role of the active site residue E121 of E. coli L-aspartate oxidase
PMID 20600565doi:10.1016/j.biochi.2010.06.015Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (30 values) from this paper: L-aspartate oxidase (LASPO, NadB) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| L-aspartate oxidase (LASPO, NadB) | Escherichia coli | (2S,3R)-2-amino-3-hydroxybutanedioic acid (3-hydroxy-erythro-L-aspartate) | 0.173 | 4.7 | 0.03688 | pH 8, 25 °C | Open record: L-aspartate oxidase (LASPO, NadB), (2S,3R)-2-amino-3-hydroxybutanedioic acid (3-hydroxy-erythro-L-aspartate) |
| L-aspartate oxidase (LASPO, NadB) | Escherichia coli | L-aspartate | 0.667 | 4.9 | 0.1361 | pH 8, 25 °C | Open record: L-aspartate oxidase (LASPO, NadB), L-aspartate |
| L-aspartate oxidase (LASPO, NadB) | Escherichia coli | N-acetyl-L-aspartate | 0.0035 | 17 | 2.07 × 10⁻⁴ | pH 8, 25 °C | Open record: L-aspartate oxidase (LASPO, NadB), N-acetyl-L-aspartate |
| L-aspartate oxidase (LASPO, NadB) | Escherichia coli | N-formyl-L-aspartate | 0.0077 | 3.7 | 0.002072 | pH 8, 25 °C | Open record: L-aspartate oxidase (LASPO, NadB), N-formyl-L-aspartate |
| L-aspartate oxidase (LASPO, NadB)variant E121A | Escherichia coli | fumarate | 0.173 | 0.038 | 4.561 | pH 8, 25 °C | Open record: L-aspartate oxidase (LASPO, NadB) variant E121A, fumarate |
| L-aspartate oxidase (LASPO, NadB)variant E121A | Escherichia coli | N-acetyl-L-aspartate | 0.0055 | 3.2 | 0.00172 | pH 8, 25 °C | Open record: L-aspartate oxidase (LASPO, NadB) variant E121A, N-acetyl-L-aspartate |
| L-aspartate oxidase (LASPO, NadB)variant E121A | Escherichia coli | N-formyl-L-aspartate | 0.0013 | 17.5 | 7.7 × 10⁻⁵ | pH 8, 25 °C | Open record: L-aspartate oxidase (LASPO, NadB) variant E121A, N-formyl-L-aspartate |
| L-aspartate oxidase (LASPO, NadB)variant E121D | Escherichia coli | fumarate | 0.215 | 26.8 | 0.00802 | pH 8, 25 °C | Open record: L-aspartate oxidase (LASPO, NadB) variant E121D, fumarate |
| L-aspartate oxidase (LASPO, NadB)variant E121K | Escherichia coli | fumarate | 0.223 | 2.9 | 0.077 | pH 8, 25 °C | Open record: L-aspartate oxidase (LASPO, NadB) variant E121K, fumarate |
| L-aspartate oxidase (LASPO, NadB)variant E121Q | Escherichia coli | fumarate | 0.202 | 0.61 | 0.3306 | pH 8, 25 °C | Open record: L-aspartate oxidase (LASPO, NadB) variant E121Q, fumarate |