Values CatLog read from this paper
From alcohol dehydrogenase to a "one-way" carbonyl reductase by active-site redesign: a mechanistic study of mannitol 2-dehydrogenase from pseudomonas fluorescens
PMID 20639204doi:10.1074/jbc.m110.108993Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 26 records (55 values) from this paper: mannitol 2-dehydrogenase (PfM2DH) from Pseudomonas fluorescens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| mannitol 2-dehydrogenase (PfM2DH) | Pseudomonas fluorescens | D-mannitol | 15.9 | 21 | 0.757 | pH 7.1, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH), D-mannitol |
| mannitol 2-dehydrogenase (PfM2DH) | Pseudomonas fluorescens | NAD+ | — | 0.775 | 20 | pH 7.1, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH), NAD+ |
| mannitol 2-dehydrogenase (PfM2DH)variant N191D | Pseudomonas fluorescens | D-fructose | 8.2 | 0.54 | 15 | pH 5.2, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N191D, D-fructose |
| mannitol 2-dehydrogenase (PfM2DH)variant N191D | Pseudomonas fluorescens | D-fructose | 2.7 | 6 | 0.45 | pH 7.1, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N191D, D-fructose |
| mannitol 2-dehydrogenase (PfM2DH)variant N191D | Pseudomonas fluorescens | D-fructoselow-pH | — | — | 19 | 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N191D, D-fructose |
| mannitol 2-dehydrogenase (PfM2DH)variant N191D | Pseudomonas fluorescens | D-fructosehigh-pH | — | — | 7 × 10⁻⁴ | 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N191D, D-fructose |
| mannitol 2-dehydrogenase (PfM2DH)variant N191D | Pseudomonas fluorescens | D-mannitol | 0.0011 | 0.32 | 0.0034 | pH 10, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N191D, D-mannitol |
| mannitol 2-dehydrogenase (PfM2DH)variant N191D | Pseudomonas fluorescens | D-mannitol | 5.5 × 10⁻⁴ | 9 | 6.1 × 10⁻⁵ | pH 7.1, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N191D, D-mannitol |
| mannitol 2-dehydrogenase (PfM2DH)variant N191D | Pseudomonas fluorescens | D-mannitol | — | — | 0.0032 | 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N191D, D-mannitol |
| mannitol 2-dehydrogenase (PfM2DH)variant N191D | Pseudomonas fluorescens | NAD+ | — | 0.054 | 0.02 | pH 10, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N191D, NAD+ |
| mannitol 2-dehydrogenase (PfM2DH)variant N191D | Pseudomonas fluorescens | NAD+ | — | 0.231 | 0.0024 | pH 7.1, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N191D, NAD+ |
| mannitol 2-dehydrogenase (PfM2DH)variant N191D | Pseudomonas fluorescens | NADH | — | 0.016 | 510 | pH 5.2, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N191D, NADH |
| mannitol 2-dehydrogenase (PfM2DH)variant N191D | Pseudomonas fluorescens | NADH | — | 0.008 | 340 | pH 7.1, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N191D, NADH |
| mannitol 2-dehydrogenase (PfM2DH)variant N191D/N300D | Pseudomonas fluorescens | D-fructose | — | — | 0.0074 | pH 5.2, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N191D/N300D, D-fructose |
| mannitol 2-dehydrogenase (PfM2DH)variant N191D/N300D | Pseudomonas fluorescens | NADH | 0.15 | 0.011 | 15 | pH 5.2, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N191D/N300D, NADH |
| mannitol 2-dehydrogenase (PfM2DH)variant N300D | Pseudomonas fluorescens | D-fructose | 0.8 | 3.9 | 0.205 | pH 5.2, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N300D, D-fructose |
| mannitol 2-dehydrogenase (PfM2DH)variant N300D | Pseudomonas fluorescens | D-fructose | 0.9 | 22 | 0.041 | pH 6.8, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N300D, D-fructose |
| mannitol 2-dehydrogenase (PfM2DH)variant N300D | Pseudomonas fluorescens | D-fructosehigh-pH | — | — | 7 × 10⁻⁴ | 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N300D, D-fructose |
| mannitol 2-dehydrogenase (PfM2DH)variant N300D | Pseudomonas fluorescens | D-fructoselow-pH | — | — | 0.211 | 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N300D, D-fructose |
| mannitol 2-dehydrogenase (PfM2DH)variant N300D | Pseudomonas fluorescens | D-mannitol | 0.0015 | 0.3 | 0.0049 | pH 10, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N300D, D-mannitol |
| mannitol 2-dehydrogenase (PfM2DH)variant N300D | Pseudomonas fluorescens | D-mannitol | 0.0014 | 93 | 1.5 × 10⁻⁵ | pH 6.8, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N300D, D-mannitol |
| mannitol 2-dehydrogenase (PfM2DH)variant N300D | Pseudomonas fluorescens | D-mannitol | — | — | 0.008 | 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N300D, D-mannitol |
| mannitol 2-dehydrogenase (PfM2DH)variant N300D | Pseudomonas fluorescens | NAD+ | — | 0.074 | 0.02 | pH 10, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N300D, NAD+ |
| mannitol 2-dehydrogenase (PfM2DH)variant N300D | Pseudomonas fluorescens | NAD+ | — | 0.001 | 1.4 | pH 6.8, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N300D, NAD+ |
| mannitol 2-dehydrogenase (PfM2DH)variant N300D | Pseudomonas fluorescens | NADH | — | 0.009 | 89 | pH 5.2, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N300D, NADH |
| mannitol 2-dehydrogenase (PfM2DH)variant N300D | Pseudomonas fluorescens | NADH | — | 0.02 | 45 | pH 6.8, 25 °C | Open record: mannitol 2-dehydrogenase (PfM2DH) variant N300D, NADH |