Values CatLog read from this paper
Structurally informed site-directed mutagenesis of a stereochemically promiscuous aldolase to afford stereochemically complementary biocatalysts
PMID 20684556doi:10.1021/ja104412aOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 6 records (12 values) from this paper: 2-keto-3-deoxygluconate aldolase (KDG aldolase) from Sulfolobus solfataricus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 6 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 2-keto-3-deoxygluconate aldolase (KDG aldolase) | Sulfolobus solfataricus | D-glyceraldehyde | 5.47 | 5.3 | 1.0 calc. | 70 °C | Open record: 2-keto-3-deoxygluconate aldolase (KDG aldolase), D-glyceraldehyde |
| 2-keto-3-deoxygluconate aldolase (KDG aldolase) | Sulfolobus solfataricus | pyruvate | 4.57 | 1.1 | 4.2 calc. | 70 °C | Open record: 2-keto-3-deoxygluconate aldolase (KDG aldolase), pyruvate |
| 2-keto-3-deoxygluconate aldolase (KDG aldolase)variant T157F/Y132V (KDGlu Mutant-2) | Sulfolobus solfataricus | D-glyceraldehyde | 0.057 | 8 | 0.0071 calc. | 70 °C | Open record: 2-keto-3-deoxygluconate aldolase (KDG aldolase) variant T157F/Y132V (KDGlu Mutant-2), D-glyceraldehyde |
| 2-keto-3-deoxygluconate aldolase (KDG aldolase)variant T157F/Y132V (KDGlu Mutant-2) | Sulfolobus solfataricus | pyruvate | 0.075 | 4.1 | 0.018 calc. | 70 °C | Open record: 2-keto-3-deoxygluconate aldolase (KDG aldolase) variant T157F/Y132V (KDGlu Mutant-2), pyruvate |
| 2-keto-3-deoxygluconate aldolase (KDG aldolase)variant T157V/A198L/D181Q (KDGal Mutant-1) | Sulfolobus solfataricus | D-glyceraldehyde | 0.072 | 9.1 | 0.0079 calc. | 70 °C | Open record: 2-keto-3-deoxygluconate aldolase (KDG aldolase) variant T157V/A198L/D181Q (KDGal Mutant-1), D-glyceraldehyde |
| 2-keto-3-deoxygluconate aldolase (KDG aldolase)variant T157V/A198L/D181Q (KDGal Mutant-1) | Sulfolobus solfataricus | pyruvate | 0.067 | 39.6 | 0.0017 calc. | 70 °C | Open record: 2-keto-3-deoxygluconate aldolase (KDG aldolase) variant T157V/A198L/D181Q (KDGal Mutant-1), pyruvate |