Values CatLog read from this paper
Crystal structure of an Exo-1,5-α-L-arabinofuranosidase from Streptomyces avermitilis provides insights into the mechanism of substrate discrimination between exo- and endo-type enzymes in…
PMID 20739278doi:10.1074/jbc.m110.164251Open in Browse
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CatLog read 17 records (29 values) from this paper: exo-1,5-α-L-arabinofuranosidase (SaAraf43A) from Streptomyces avermitilis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| exo-1,5-α-L-arabinofuranosidase (SaAraf43A) | Streptomyces avermitilis | methyl 5-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside | — | — | 63.6 | pH 6, 30 °C | Open record: exo-1,5-α-L-arabinofuranosidase (SaAraf43A), methyl 5-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside |
| exo-1,5-α-L-arabinofuranosidase (SaAraf43A) | Streptomyces avermitilis | p-nitrophenyl α-L-arabinofuranoside | 0.108 | 0.5 | 0.217 | pH 6, 30 °C | Open record: exo-1,5-α-L-arabinofuranosidase (SaAraf43A), p-nitrophenyl α-L-arabinofuranoside |
| exo-1,5-α-L-arabinofuranosidase (SaAraf43A) | Streptomyces avermitilis | α-1,5-L-arabinofuranobiose | — | — | 7.1 | pH 6, 30 °C | Open record: exo-1,5-α-L-arabinofuranosidase (SaAraf43A), α-1,5-L-arabinofuranobiose |
| exo-1,5-α-L-arabinofuranosidase (SaAraf43A) | Streptomyces avermitilis | α-1,5-L-arabinofuranopentaose | — | — | 163.6 | pH 6, 30 °C | Open record: exo-1,5-α-L-arabinofuranosidase (SaAraf43A), α-1,5-L-arabinofuranopentaose |
| exo-1,5-α-L-arabinofuranosidase (SaAraf43A) | Streptomyces avermitilis | α-1,5-L-arabinofuranotetraose | — | — | 173.7 | pH 6, 30 °C | Open record: exo-1,5-α-L-arabinofuranosidase (SaAraf43A), α-1,5-L-arabinofuranotetraose |
| exo-1,5-α-L-arabinofuranosidase (SaAraf43A) | Streptomyces avermitilis | α-1,5-L-arabinofuranotriose | — | — | 92.6 | pH 6, 30 °C | Open record: exo-1,5-α-L-arabinofuranosidase (SaAraf43A), α-1,5-L-arabinofuranotriose |
| exo-1,5-α-L-arabinofuranosidase (SaAraf43A)variant D20N | Streptomyces avermitilis | p-nitrophenyl α-L-arabinofuranoside | 0.0017 | 0.001 | 1.5 × 10⁻⁴ | pH 6, 30 °C | Open record: exo-1,5-α-L-arabinofuranosidase (SaAraf43A) variant D20N, p-nitrophenyl α-L-arabinofuranoside |
| exo-1,5-α-L-arabinofuranosidase (SaAraf43A)variant L289A | Streptomyces avermitilis | methyl 2-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside | — | — | 0.0083 | pH 6, 30 °C | Open record: exo-1,5-α-L-arabinofuranosidase (SaAraf43A) variant L289A, methyl 2-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside |
| exo-1,5-α-L-arabinofuranosidase (SaAraf43A)variant L289A | Streptomyces avermitilis | methyl 5-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside | — | — | 0.067 | pH 6, 30 °C | Open record: exo-1,5-α-L-arabinofuranosidase (SaAraf43A) variant L289A, methyl 5-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside |
| exo-1,5-α-L-arabinofuranosidase (SaAraf43A)variant L289A | Streptomyces avermitilis | p-nitrophenyl α-L-arabinofuranoside | 0.62 | 40.7 | 0.015 | pH 6, 30 °C | Open record: exo-1,5-α-L-arabinofuranosidase (SaAraf43A) variant L289A, p-nitrophenyl α-L-arabinofuranoside |
| exo-1,5-α-L-arabinofuranosidase (SaAraf43A)variant N159A | Streptomyces avermitilis | methyl 5-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside | — | — | 1.78 | pH 6, 30 °C | Open record: exo-1,5-α-L-arabinofuranosidase (SaAraf43A) variant N159A, methyl 5-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside |
| exo-1,5-α-L-arabinofuranosidase (SaAraf43A)variant N159A | Streptomyces avermitilis | p-nitrophenyl α-L-arabinofuranoside | 0.558 | 1.7 | 0.328 | pH 6, 30 °C | Open record: exo-1,5-α-L-arabinofuranosidase (SaAraf43A) variant N159A, p-nitrophenyl α-L-arabinofuranoside |
| exo-1,5-α-L-arabinofuranosidase (SaAraf43A)variant N159L | Streptomyces avermitilis | methyl 5-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside | — | — | 0.18 | pH 6, 30 °C | Open record: exo-1,5-α-L-arabinofuranosidase (SaAraf43A) variant N159L, methyl 5-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside |
| exo-1,5-α-L-arabinofuranosidase (SaAraf43A)variant N159L | Streptomyces avermitilis | p-nitrophenyl α-L-arabinofuranoside | 4.553 | 1.1 | 4.14 | pH 6, 30 °C | Open record: exo-1,5-α-L-arabinofuranosidase (SaAraf43A) variant N159L, p-nitrophenyl α-L-arabinofuranoside |
| exo-1,5-α-L-arabinofuranosidase (SaAraf43A)variant Y192A | Streptomyces avermitilis | methyl 2-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside | — | — | 0.0083 | pH 6, 30 °C | Open record: exo-1,5-α-L-arabinofuranosidase (SaAraf43A) variant Y192A, methyl 2-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside |
| exo-1,5-α-L-arabinofuranosidase (SaAraf43A)variant Y192A | Streptomyces avermitilis | methyl 5-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside | — | — | 11.15 | pH 6, 30 °C | Open record: exo-1,5-α-L-arabinofuranosidase (SaAraf43A) variant Y192A, methyl 5-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside |
| exo-1,5-α-L-arabinofuranosidase (SaAraf43A)variant Y192A | Streptomyces avermitilis | p-nitrophenyl α-L-arabinofuranoside | 1.065 | 11.5 | 0.093 | pH 6, 30 °C | Open record: exo-1,5-α-L-arabinofuranosidase (SaAraf43A) variant Y192A, p-nitrophenyl α-L-arabinofuranoside |